Effects of dietary xylooligosaccharide on growth, digestive enzymes activity, intestinal morphology, and the expression of inflammatory cytokines and tight junctions genes in triploid Oncorhynchus mykiss fed a low fishmeal diet

The purpose of this study was to show how dietary xylooligosaccharides (XOS) affect growth and intestinal digestive enzyme activity, histology, inflammatory cytokines, barrier function gene expression, and microbiota of juvenile rainbow trout (Oncorhynchus mykiss). Different diets (G1, G2, G3, G4, a...

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Autores principales:	Chang’an Wang, Zhe Xu, Shaoxia Lu, Haibo Jiang, Jinnan Li, Liansheng Wang, Ze Fan, Di Wu, Yuanyuan Zhang, Shicheng Han, Yang Liu, Hongbai Liu, Zhuang Li
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Rainbow trout Prebiotic Intestine microbiota Disease resistance Aquaculture. Fisheries. Angling SH1-691
Acceso en línea:	https://doaj.org/article/c36024f54a3a4e39bb7e1fe4684c410d
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spelling	oai:doaj.org-article:c36024f54a3a4e39bb7e1fe4684c410d2021-11-16T04:10:49ZEffects of dietary xylooligosaccharide on growth, digestive enzymes activity, intestinal morphology, and the expression of inflammatory cytokines and tight junctions genes in triploid Oncorhynchus mykiss fed a low fishmeal diet2352-513410.1016/j.aqrep.2021.100941https://doaj.org/article/c36024f54a3a4e39bb7e1fe4684c410d2022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2352513421003586https://doaj.org/toc/2352-5134The purpose of this study was to show how dietary xylooligosaccharides (XOS) affect growth and intestinal digestive enzyme activity, histology, inflammatory cytokines, barrier function gene expression, and microbiota of juvenile rainbow trout (Oncorhynchus mykiss). Different diets (G1, G2, G3, G4, and G5) containing graded levels of XOS (0, 2.5, 5.0, 7.5, and 10.0 g/kg) were fed to triplicate groups of 30 fish (initial average weight 20.85 ± 0.48 g) for 56 days. The fish fed 10 g/kg XOS had significantly improved weight gain (P < 0.05). Intestinal lipase and amylase activity were significantly improved (P < 0.05). Intestinal histology in the height of the villi was also significantly improved (P < 0.05). TNF-alpha factor (TNF-α) and interleukin 6 (IL-6) were significantly lower in G4–G5 than that in G1 (P < 0.05); Interleukin 10 (IL-10), claudin-1, and ZO-1 showed the opposite trend. Dietary XOS altered the intestinal bacterial composition. At the phylum level, dietary XOS supplementation resulted in a decrease in abundance of the Proteobacteria in G2 and G5 treatments. At the genus level, compared to the control group, the genus Lactobacillus showed an increase with dietary XOS supplementation in G3–G5 treatments. Overall, feeding juvenile triploid O. mykiss 5.0–10.0 g/kg XOS improved growth and intestinal health by promoting intestinal development, limiting intestinal damage and inflammation, and regulating the community structure of the intestinal microbiota.Chang’an WangZhe XuShaoxia LuHaibo JiangJinnan LiLiansheng WangZe FanDi WuYuanyuan ZhangShicheng HanYang LiuHongbai LiuZhuang LiElsevierarticleRainbow troutPrebioticIntestine microbiotaDisease resistanceAquaculture. Fisheries. AnglingSH1-691ENAquaculture Reports, Vol 22, Iss , Pp 100941- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Rainbow trout Prebiotic Intestine microbiota Disease resistance Aquaculture. Fisheries. Angling SH1-691
spellingShingle	Rainbow trout Prebiotic Intestine microbiota Disease resistance Aquaculture. Fisheries. Angling SH1-691 Chang’an Wang Zhe Xu Shaoxia Lu Haibo Jiang Jinnan Li Liansheng Wang Ze Fan Di Wu Yuanyuan Zhang Shicheng Han Yang Liu Hongbai Liu Zhuang Li Effects of dietary xylooligosaccharide on growth, digestive enzymes activity, intestinal morphology, and the expression of inflammatory cytokines and tight junctions genes in triploid Oncorhynchus mykiss fed a low fishmeal diet
description	The purpose of this study was to show how dietary xylooligosaccharides (XOS) affect growth and intestinal digestive enzyme activity, histology, inflammatory cytokines, barrier function gene expression, and microbiota of juvenile rainbow trout (Oncorhynchus mykiss). Different diets (G1, G2, G3, G4, and G5) containing graded levels of XOS (0, 2.5, 5.0, 7.5, and 10.0 g/kg) were fed to triplicate groups of 30 fish (initial average weight 20.85 ± 0.48 g) for 56 days. The fish fed 10 g/kg XOS had significantly improved weight gain (P < 0.05). Intestinal lipase and amylase activity were significantly improved (P < 0.05). Intestinal histology in the height of the villi was also significantly improved (P < 0.05). TNF-alpha factor (TNF-α) and interleukin 6 (IL-6) were significantly lower in G4–G5 than that in G1 (P < 0.05); Interleukin 10 (IL-10), claudin-1, and ZO-1 showed the opposite trend. Dietary XOS altered the intestinal bacterial composition. At the phylum level, dietary XOS supplementation resulted in a decrease in abundance of the Proteobacteria in G2 and G5 treatments. At the genus level, compared to the control group, the genus Lactobacillus showed an increase with dietary XOS supplementation in G3–G5 treatments. Overall, feeding juvenile triploid O. mykiss 5.0–10.0 g/kg XOS improved growth and intestinal health by promoting intestinal development, limiting intestinal damage and inflammation, and regulating the community structure of the intestinal microbiota.
format	article
author	Chang’an Wang Zhe Xu Shaoxia Lu Haibo Jiang Jinnan Li Liansheng Wang Ze Fan Di Wu Yuanyuan Zhang Shicheng Han Yang Liu Hongbai Liu Zhuang Li
author_facet	Chang’an Wang Zhe Xu Shaoxia Lu Haibo Jiang Jinnan Li Liansheng Wang Ze Fan Di Wu Yuanyuan Zhang Shicheng Han Yang Liu Hongbai Liu Zhuang Li
author_sort	Chang’an Wang
title	Effects of dietary xylooligosaccharide on growth, digestive enzymes activity, intestinal morphology, and the expression of inflammatory cytokines and tight junctions genes in triploid Oncorhynchus mykiss fed a low fishmeal diet
title_short	Effects of dietary xylooligosaccharide on growth, digestive enzymes activity, intestinal morphology, and the expression of inflammatory cytokines and tight junctions genes in triploid Oncorhynchus mykiss fed a low fishmeal diet
title_full	Effects of dietary xylooligosaccharide on growth, digestive enzymes activity, intestinal morphology, and the expression of inflammatory cytokines and tight junctions genes in triploid Oncorhynchus mykiss fed a low fishmeal diet
title_fullStr	Effects of dietary xylooligosaccharide on growth, digestive enzymes activity, intestinal morphology, and the expression of inflammatory cytokines and tight junctions genes in triploid Oncorhynchus mykiss fed a low fishmeal diet
title_full_unstemmed	Effects of dietary xylooligosaccharide on growth, digestive enzymes activity, intestinal morphology, and the expression of inflammatory cytokines and tight junctions genes in triploid Oncorhynchus mykiss fed a low fishmeal diet
title_sort	effects of dietary xylooligosaccharide on growth, digestive enzymes activity, intestinal morphology, and the expression of inflammatory cytokines and tight junctions genes in triploid oncorhynchus mykiss fed a low fishmeal diet
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/c36024f54a3a4e39bb7e1fe4684c410d
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Effects of dietary xylooligosaccharide on growth, digestive enzymes activity, intestinal morphology, and the expression of inflammatory cytokines and tight junctions genes in triploid Oncorhynchus mykiss fed a low fishmeal diet

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