Blast-induced temporal alterations in blood–brain barrier properties in a rodent model

Abstract The consequences of blast-induced traumatic brain injury (bTBI) on the blood–brain barrier (BBB) and components of the neurovascular unit are an area of active research. In this study we assessed the time course of BBB integrity in anesthetized rats exposed to a single blast overpressure of...

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Autores principales:	Usmah Kawoos, Rania Abutarboush, Ming Gu, Ye Chen, Jonathan K. Statz, Samantha Y. Goodrich, Stephen T. Ahlers
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c36ef5d7507b4e7380b7d8605d1e2f3a
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spelling	oai:doaj.org-article:c36ef5d7507b4e7380b7d8605d1e2f3a2021-12-02T16:30:18ZBlast-induced temporal alterations in blood–brain barrier properties in a rodent model10.1038/s41598-021-84730-82045-2322https://doaj.org/article/c36ef5d7507b4e7380b7d8605d1e2f3a2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84730-8https://doaj.org/toc/2045-2322Abstract The consequences of blast-induced traumatic brain injury (bTBI) on the blood–brain barrier (BBB) and components of the neurovascular unit are an area of active research. In this study we assessed the time course of BBB integrity in anesthetized rats exposed to a single blast overpressure of 130 kPa (18.9 PSI). BBB permeability was measured in vivo via intravital microscopy by imaging extravasation of fluorescently labeled tracers (40 kDa and 70 kDa molecular weight) through the pial microvasculature into brain parenchyma at 2–3 h, 1, 3, 14, or 28 days after the blast exposure. BBB structural changes were assessed by immunostaining and molecular assays. At 2–3 h and 1 day after blast exposure, significant increases in the extravasation of the 40 kDa but not the 70 kDa tracers were observed, along with differential reductions in the expression of tight junction proteins (occludin, claudin-5, zona occluden-1) and increase in the levels of the astrocytic water channel protein, AQP-4, and matrix metalloprotease, MMP-9. Nearly all of these measures were normalized by day 3 and maintained up to 28 days post exposure. These data demonstrate that blast-induced changes in BBB permeability are closely coupled to structural and functional components of the BBB.Usmah KawoosRania AbutarboushMing GuYe ChenJonathan K. StatzSamantha Y. GoodrichStephen T. AhlersNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Usmah Kawoos Rania Abutarboush Ming Gu Ye Chen Jonathan K. Statz Samantha Y. Goodrich Stephen T. Ahlers Blast-induced temporal alterations in blood–brain barrier properties in a rodent model
description	Abstract The consequences of blast-induced traumatic brain injury (bTBI) on the blood–brain barrier (BBB) and components of the neurovascular unit are an area of active research. In this study we assessed the time course of BBB integrity in anesthetized rats exposed to a single blast overpressure of 130 kPa (18.9 PSI). BBB permeability was measured in vivo via intravital microscopy by imaging extravasation of fluorescently labeled tracers (40 kDa and 70 kDa molecular weight) through the pial microvasculature into brain parenchyma at 2–3 h, 1, 3, 14, or 28 days after the blast exposure. BBB structural changes were assessed by immunostaining and molecular assays. At 2–3 h and 1 day after blast exposure, significant increases in the extravasation of the 40 kDa but not the 70 kDa tracers were observed, along with differential reductions in the expression of tight junction proteins (occludin, claudin-5, zona occluden-1) and increase in the levels of the astrocytic water channel protein, AQP-4, and matrix metalloprotease, MMP-9. Nearly all of these measures were normalized by day 3 and maintained up to 28 days post exposure. These data demonstrate that blast-induced changes in BBB permeability are closely coupled to structural and functional components of the BBB.
format	article
author	Usmah Kawoos Rania Abutarboush Ming Gu Ye Chen Jonathan K. Statz Samantha Y. Goodrich Stephen T. Ahlers
author_facet	Usmah Kawoos Rania Abutarboush Ming Gu Ye Chen Jonathan K. Statz Samantha Y. Goodrich Stephen T. Ahlers
author_sort	Usmah Kawoos
title	Blast-induced temporal alterations in blood–brain barrier properties in a rodent model
title_short	Blast-induced temporal alterations in blood–brain barrier properties in a rodent model
title_full	Blast-induced temporal alterations in blood–brain barrier properties in a rodent model
title_fullStr	Blast-induced temporal alterations in blood–brain barrier properties in a rodent model
title_full_unstemmed	Blast-induced temporal alterations in blood–brain barrier properties in a rodent model
title_sort	blast-induced temporal alterations in blood–brain barrier properties in a rodent model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c36ef5d7507b4e7380b7d8605d1e2f3a
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Blast-induced temporal alterations in blood–brain barrier properties in a rodent model

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