Solonamide B inhibits quorum sensing and reduces Staphylococcus aureus mediated killing of human neutrophils.

Methicillin-resistant Staphylococcus aureus (MRSA) continues to be a serious human pathogen, and particularly the spread of community associated (CA)-MRSA strains such as USA300 is a concern, as these strains can cause severe infections in otherwise healthy adults. Recently, we reported that a cyclo...

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Autores principales:	Anita Nielsen, Maria Månsson, Martin S Bojer, Lone Gram, Thomas O Larsen, Richard P Novick, Dorte Frees, Hanne Frøkiær, Hanne Ingmer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c37608bfdaa5420f836fb983273c266b
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spelling	oai:doaj.org-article:c37608bfdaa5420f836fb983273c266b2021-11-18T08:38:26ZSolonamide B inhibits quorum sensing and reduces Staphylococcus aureus mediated killing of human neutrophils.1932-620310.1371/journal.pone.0084992https://doaj.org/article/c37608bfdaa5420f836fb983273c266b2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24416329/?tool=EBIhttps://doaj.org/toc/1932-6203Methicillin-resistant Staphylococcus aureus (MRSA) continues to be a serious human pathogen, and particularly the spread of community associated (CA)-MRSA strains such as USA300 is a concern, as these strains can cause severe infections in otherwise healthy adults. Recently, we reported that a cyclodepsipeptide termed Solonamide B isolated from the marine bacterium, Photobacterium halotolerans strongly reduces expression of RNAIII, the effector molecule of the agr quorum sensing system. Here we show that Solonamide B interferes with the binding of S. aureus autoinducing peptides (AIPs) to sensor histidine kinase, AgrC, of the agr two-component system. The hypervirulence of USA300 has been linked to increased expression of central virulence factors like α-hemolysin and the phenol soluble modulins (PSMs). Importantly, in strain USA300 Solonamide B dramatically reduced the activity of α-hemolysin and the transcription of psma encoding PSMs with an 80% reduction in toxicity of supernatants towards human neutrophils and rabbit erythrocytes. To our knowledge this is the first report of a compound produced naturally by a Gram-negative marine bacterium that interferes with agr and affects both RNAIII and AgrA controlled virulence gene expression in S. aureus.Anita NielsenMaria MånssonMartin S BojerLone GramThomas O LarsenRichard P NovickDorte FreesHanne FrøkiærHanne IngmerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e84992 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anita Nielsen Maria Månsson Martin S Bojer Lone Gram Thomas O Larsen Richard P Novick Dorte Frees Hanne Frøkiær Hanne Ingmer Solonamide B inhibits quorum sensing and reduces Staphylococcus aureus mediated killing of human neutrophils.
description	Methicillin-resistant Staphylococcus aureus (MRSA) continues to be a serious human pathogen, and particularly the spread of community associated (CA)-MRSA strains such as USA300 is a concern, as these strains can cause severe infections in otherwise healthy adults. Recently, we reported that a cyclodepsipeptide termed Solonamide B isolated from the marine bacterium, Photobacterium halotolerans strongly reduces expression of RNAIII, the effector molecule of the agr quorum sensing system. Here we show that Solonamide B interferes with the binding of S. aureus autoinducing peptides (AIPs) to sensor histidine kinase, AgrC, of the agr two-component system. The hypervirulence of USA300 has been linked to increased expression of central virulence factors like α-hemolysin and the phenol soluble modulins (PSMs). Importantly, in strain USA300 Solonamide B dramatically reduced the activity of α-hemolysin and the transcription of psma encoding PSMs with an 80% reduction in toxicity of supernatants towards human neutrophils and rabbit erythrocytes. To our knowledge this is the first report of a compound produced naturally by a Gram-negative marine bacterium that interferes with agr and affects both RNAIII and AgrA controlled virulence gene expression in S. aureus.
format	article
author	Anita Nielsen Maria Månsson Martin S Bojer Lone Gram Thomas O Larsen Richard P Novick Dorte Frees Hanne Frøkiær Hanne Ingmer
author_facet	Anita Nielsen Maria Månsson Martin S Bojer Lone Gram Thomas O Larsen Richard P Novick Dorte Frees Hanne Frøkiær Hanne Ingmer
author_sort	Anita Nielsen
title	Solonamide B inhibits quorum sensing and reduces Staphylococcus aureus mediated killing of human neutrophils.
title_short	Solonamide B inhibits quorum sensing and reduces Staphylococcus aureus mediated killing of human neutrophils.
title_full	Solonamide B inhibits quorum sensing and reduces Staphylococcus aureus mediated killing of human neutrophils.
title_fullStr	Solonamide B inhibits quorum sensing and reduces Staphylococcus aureus mediated killing of human neutrophils.
title_full_unstemmed	Solonamide B inhibits quorum sensing and reduces Staphylococcus aureus mediated killing of human neutrophils.
title_sort	solonamide b inhibits quorum sensing and reduces staphylococcus aureus mediated killing of human neutrophils.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/c37608bfdaa5420f836fb983273c266b
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Solonamide B inhibits quorum sensing and reduces Staphylococcus aureus mediated killing of human neutrophils.

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