Friends or Foes? Rapid Determination of Dissimilar Colistin and Ciprofloxacin Antagonism of <i>Pseudomonas aeruginosa</i> Phages

Phage therapy is a century-old technique employing viruses (phages) to treat bacterial infections, and in the clinic it is often used in combination with antibiotics. Antibiotics, however, interfere with critical bacterial metabolic activities that can be required by phages. Explicit testing of anti...

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Autores principales:	Katarzyna M. Danis-Wlodarczyk, Alice Cai, Anna Chen, Marissa R. Gittrich, Matthew B. Sullivan, Daniel J. Wozniak, Stephen T. Abedon
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antibacterial therapy bacteriophage therapy ciprofloxacin colistin phage PEV2 phage ΦKMV Medicine R Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/c38b403faf37400cafd313e8796411db
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spelling	oai:doaj.org-article:c38b403faf37400cafd313e8796411db2021-11-25T18:39:50ZFriends or Foes? Rapid Determination of Dissimilar Colistin and Ciprofloxacin Antagonism of <i>Pseudomonas aeruginosa</i> Phages10.3390/ph141111621424-8247https://doaj.org/article/c38b403faf37400cafd313e8796411db2021-11-01T00:00:00Zhttps://www.mdpi.com/1424-8247/14/11/1162https://doaj.org/toc/1424-8247Phage therapy is a century-old technique employing viruses (phages) to treat bacterial infections, and in the clinic it is often used in combination with antibiotics. Antibiotics, however, interfere with critical bacterial metabolic activities that can be required by phages. Explicit testing of antibiotic antagonism of phage infection activities, though, is not a common feature of phage therapy studies. Here we use optical density-based ‘lysis-profile’ assays to assess the impact of two antibiotics, colistin and ciprofloxacin, on the bactericidal, bacteriolytic, and new-virion-production activities of three <i>Pseudomonas aeruginosa</i> phages. Though phages and antibiotics in combination are more potent in killing <i>P. aeruginosa</i> than either acting alone, colistin nevertheless substantially interferes with phage bacteriolytic and virion-production activities even at its minimum inhibitory concentration (1× MIC). Ciprofloxacin, by contrast, has little anti-phage impact at 1× or 3× MIC. We corroborate these results with more traditional measures, particularly colony-forming units, plaque-forming units, and one-step growth experiments. Our results suggest that ciprofloxacin could be useful as a concurrent phage therapy co-treatment especially when phage replication is required for treatment success. Lysis-profile assays also appear to be useful, fast, and high-throughput means of assessing antibiotic antagonism of phage infection activities.Katarzyna M. Danis-WlodarczykAlice CaiAnna ChenMarissa R. GittrichMatthew B. SullivanDaniel J. WozniakStephen T. AbedonMDPI AGarticleantibacterial therapybacteriophage therapyciprofloxacincolistinphage PEV2phage ΦKMVMedicineRPharmacy and materia medicaRS1-441ENPharmaceuticals, Vol 14, Iss 1162, p 1162 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibacterial therapy bacteriophage therapy ciprofloxacin colistin phage PEV2 phage ΦKMV Medicine R Pharmacy and materia medica RS1-441
spellingShingle	antibacterial therapy bacteriophage therapy ciprofloxacin colistin phage PEV2 phage ΦKMV Medicine R Pharmacy and materia medica RS1-441 Katarzyna M. Danis-Wlodarczyk Alice Cai Anna Chen Marissa R. Gittrich Matthew B. Sullivan Daniel J. Wozniak Stephen T. Abedon Friends or Foes? Rapid Determination of Dissimilar Colistin and Ciprofloxacin Antagonism of <i>Pseudomonas aeruginosa</i> Phages
description	Phage therapy is a century-old technique employing viruses (phages) to treat bacterial infections, and in the clinic it is often used in combination with antibiotics. Antibiotics, however, interfere with critical bacterial metabolic activities that can be required by phages. Explicit testing of antibiotic antagonism of phage infection activities, though, is not a common feature of phage therapy studies. Here we use optical density-based ‘lysis-profile’ assays to assess the impact of two antibiotics, colistin and ciprofloxacin, on the bactericidal, bacteriolytic, and new-virion-production activities of three <i>Pseudomonas aeruginosa</i> phages. Though phages and antibiotics in combination are more potent in killing <i>P. aeruginosa</i> than either acting alone, colistin nevertheless substantially interferes with phage bacteriolytic and virion-production activities even at its minimum inhibitory concentration (1× MIC). Ciprofloxacin, by contrast, has little anti-phage impact at 1× or 3× MIC. We corroborate these results with more traditional measures, particularly colony-forming units, plaque-forming units, and one-step growth experiments. Our results suggest that ciprofloxacin could be useful as a concurrent phage therapy co-treatment especially when phage replication is required for treatment success. Lysis-profile assays also appear to be useful, fast, and high-throughput means of assessing antibiotic antagonism of phage infection activities.
format	article
author	Katarzyna M. Danis-Wlodarczyk Alice Cai Anna Chen Marissa R. Gittrich Matthew B. Sullivan Daniel J. Wozniak Stephen T. Abedon
author_facet	Katarzyna M. Danis-Wlodarczyk Alice Cai Anna Chen Marissa R. Gittrich Matthew B. Sullivan Daniel J. Wozniak Stephen T. Abedon
author_sort	Katarzyna M. Danis-Wlodarczyk
title	Friends or Foes? Rapid Determination of Dissimilar Colistin and Ciprofloxacin Antagonism of <i>Pseudomonas aeruginosa</i> Phages
title_short	Friends or Foes? Rapid Determination of Dissimilar Colistin and Ciprofloxacin Antagonism of <i>Pseudomonas aeruginosa</i> Phages
title_full	Friends or Foes? Rapid Determination of Dissimilar Colistin and Ciprofloxacin Antagonism of <i>Pseudomonas aeruginosa</i> Phages
title_fullStr	Friends or Foes? Rapid Determination of Dissimilar Colistin and Ciprofloxacin Antagonism of <i>Pseudomonas aeruginosa</i> Phages
title_full_unstemmed	Friends or Foes? Rapid Determination of Dissimilar Colistin and Ciprofloxacin Antagonism of <i>Pseudomonas aeruginosa</i> Phages
title_sort	friends or foes? rapid determination of dissimilar colistin and ciprofloxacin antagonism of <i>pseudomonas aeruginosa</i> phages
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c38b403faf37400cafd313e8796411db
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Friends or Foes? Rapid Determination of Dissimilar Colistin and Ciprofloxacin Antagonism of <i>Pseudomonas aeruginosa</i> Phages

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