Direct production of a genetically-encoded immobilized biodiesel catalyst

Abstract The use of immobilized enzymes as biocatalysts has great potential to improve the efficiency and environmental sustainability of many industrial processes. Here, we report a novel approach that allows for the direct production of a highly active immobilized lipase within the bacterium Bacil...

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Autores principales:	Bradley S. Heater, Marianne M. Lee, Michael K. Chan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c38eb2df5dcc40cdb2c520c9462177cd
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spelling	oai:doaj.org-article:c38eb2df5dcc40cdb2c520c9462177cd2021-12-02T15:08:17ZDirect production of a genetically-encoded immobilized biodiesel catalyst10.1038/s41598-018-31213-y2045-2322https://doaj.org/article/c38eb2df5dcc40cdb2c520c9462177cd2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-31213-yhttps://doaj.org/toc/2045-2322Abstract The use of immobilized enzymes as biocatalysts has great potential to improve the efficiency and environmental sustainability of many industrial processes. Here, we report a novel approach that allows for the direct production of a highly active immobilized lipase within the bacterium Bacillus thuringiensis. Cry3Aa-lipA crystals were generated by genetically fusing Bacillus subtilis lipase A to Cry3Aa, a protein that naturally forms crystals in the bacteria. The crystal framework significantly stabilized the lipase against denaturation in organic solvents and high temperatures, resulting in a highly efficient fusion crystal that could catalyze the conversion of triacylglycerols to fatty acid methyl ester biodiesel to near-completion over 10 cycles. The simplicity and robustness of the Cry-fusion crystal (CFC) immobilization system could make it an appealing platform for generating industrial biocatalysts for multiple bioprocesses.Bradley S. HeaterMarianne M. LeeMichael K. ChanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bradley S. Heater Marianne M. Lee Michael K. Chan Direct production of a genetically-encoded immobilized biodiesel catalyst
description	Abstract The use of immobilized enzymes as biocatalysts has great potential to improve the efficiency and environmental sustainability of many industrial processes. Here, we report a novel approach that allows for the direct production of a highly active immobilized lipase within the bacterium Bacillus thuringiensis. Cry3Aa-lipA crystals were generated by genetically fusing Bacillus subtilis lipase A to Cry3Aa, a protein that naturally forms crystals in the bacteria. The crystal framework significantly stabilized the lipase against denaturation in organic solvents and high temperatures, resulting in a highly efficient fusion crystal that could catalyze the conversion of triacylglycerols to fatty acid methyl ester biodiesel to near-completion over 10 cycles. The simplicity and robustness of the Cry-fusion crystal (CFC) immobilization system could make it an appealing platform for generating industrial biocatalysts for multiple bioprocesses.
format	article
author	Bradley S. Heater Marianne M. Lee Michael K. Chan
author_facet	Bradley S. Heater Marianne M. Lee Michael K. Chan
author_sort	Bradley S. Heater
title	Direct production of a genetically-encoded immobilized biodiesel catalyst
title_short	Direct production of a genetically-encoded immobilized biodiesel catalyst
title_full	Direct production of a genetically-encoded immobilized biodiesel catalyst
title_fullStr	Direct production of a genetically-encoded immobilized biodiesel catalyst
title_full_unstemmed	Direct production of a genetically-encoded immobilized biodiesel catalyst
title_sort	direct production of a genetically-encoded immobilized biodiesel catalyst
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/c38eb2df5dcc40cdb2c520c9462177cd
work_keys_str_mv	AT bradleysheater directproductionofageneticallyencodedimmobilizedbiodieselcatalyst AT mariannemlee directproductionofageneticallyencodedimmobilizedbiodieselcatalyst AT michaelkchan directproductionofageneticallyencodedimmobilizedbiodieselcatalyst
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Direct production of a genetically-encoded immobilized biodiesel catalyst

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