Intravital imaging by simultaneous label-free autofluorescence-multiharmonic microscopy

Label-free and real-time visualization of the tumor microenvironment is attractive but challenging. Here the authors present an approach for simultaneous autofluorescence functional imaging and second/third harmonic generation imaging of structural features, using a single excitation source.

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Detalles Bibliográficos
Autores principales: Sixian You, Haohua Tu, Eric J. Chaney, Yi Sun, Youbo Zhao, Andrew J. Bower, Yuan-Zhi Liu, Marina Marjanovic, Saurabh Sinha, Yang Pu, Stephen A. Boppart
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/c390f51869424e149f08c881c3e09481
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Sumario:Label-free and real-time visualization of the tumor microenvironment is attractive but challenging. Here the authors present an approach for simultaneous autofluorescence functional imaging and second/third harmonic generation imaging of structural features, using a single excitation source.