The potency of actinomycetes extracts isolated from Pramuka Island, Jakarta, Indonesia as antimicrobial agents

Abstract. Setiawati S, Nuryastuti T, Sholikhak EN, Lisdiyanti P, Pratiwi SUT, Sulistiyanti TR, Ratnakomala S, Jumina, Mustofa. 2021. The potency of actinomycetes extracts isolated from Pramuka Island, Jakarta, Indonesia as antimicrobial agents. Biodiversitas 22: 1104-1111. Actinomycetes are one of t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Setiawati Setiawati, Titik Nuryastuti, Eti Nurwening Sholikhah, Puspita Lisdiyanti, Sylvia Utami Tunjung Pratiwi, TRI RATNA SULISTIYANI, SHANTI RATNAKOMALA, Jumina Jumina, Mustofa Mustofa
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	16s rrna gene, actinomycetes, antibacterial, antifungal Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c39201284ce4460cbd97b592a022f1ea
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c39201284ce4460cbd97b592a022f1ea
record_format	dspace
spelling	oai:doaj.org-article:c39201284ce4460cbd97b592a022f1ea2021-11-22T00:56:37ZThe potency of actinomycetes extracts isolated from Pramuka Island, Jakarta, Indonesia as antimicrobial agents1412-033X2085-472210.13057/biodiv/d220304https://doaj.org/article/c39201284ce4460cbd97b592a022f1ea2021-02-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5964https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Setiawati S, Nuryastuti T, Sholikhak EN, Lisdiyanti P, Pratiwi SUT, Sulistiyanti TR, Ratnakomala S, Jumina, Mustofa. 2021. The potency of actinomycetes extracts isolated from Pramuka Island, Jakarta, Indonesia as antimicrobial agents. Biodiversitas 22: 1104-1111. Actinomycetes are one of the Gram-positive bacteria which are widely distributed and produce many secondary metabolites including those known as antibiotics, antifungals, anticancer, and antimalarials agents. The secondary metabolites of actinomycetes are abundant, which include many active compounds that have been identified because of the large diversity in the actinomycetes phylum. This study aimed to identify and screen collected by Indonesian Culture Collection (InaCC) from Bojong Gede and Pramuka Island, Jakarta, Indonesia as antibacterial and antifungal agents. Primary screening was done on 16 actinomycetes isolates by well-agar diffusion method. Antimicrobial activity was tested by using micro broth dilution methods to determine minimum inhibitory concentration (MIC). Molecular identification into level genera and species was determined by 16S rRNA gene sequencing. Out of 16 actinomycetes isolates used, 4 isolates have activity against Candida albicans ATCC 10231, Staphylococcus aureus ATCC 6538, Bacillus subtilis BTCC B-612, and Escherichia coli BTCC B-614, specifically InaCC A758, InaCC A759, InaCC A760 and InaCC A765 isolates. InaCC A758 have highest antimicrobial activity against mentioned microbial with MIC value at 50 ?g/mL, 6.25 ?g/mL, 31.25 ?g/mL and 3.125 ?g/mL, respectively. Three genera were found from the samples: i.e. Streptomyces (80%), Microbispora (13%) and Nocardia (6%). Based on 16S rRNA gene identification, the active isolates of actinomycetes InaCC A758, InaCC A759, InaCC A760 and InaCC A765 were similar to Streptomyces badius, Streptomyces olivaceus, Streptomyces sanyensis, and Nocardia otitidiscaviarum, respectively. The secondary metabolites of actinomycetes extracts from Pramuka Island can be potentially developed as antifungal and antibacterial agents.Setiawati SetiawatiTitik NuryastutiEti Nurwening SholikhahPuspita LisdiyantiSylvia Utami Tunjung PratiwiTRI RATNA SULISTIYANISHANTI RATNAKOMALAJumina JuminaMustofa MustofaMBI & UNS Soloarticle16s rrna gene, actinomycetes, antibacterial, antifungalBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	16s rrna gene, actinomycetes, antibacterial, antifungal Biology (General) QH301-705.5
spellingShingle	16s rrna gene, actinomycetes, antibacterial, antifungal Biology (General) QH301-705.5 Setiawati Setiawati Titik Nuryastuti Eti Nurwening Sholikhah Puspita Lisdiyanti Sylvia Utami Tunjung Pratiwi TRI RATNA SULISTIYANI SHANTI RATNAKOMALA Jumina Jumina Mustofa Mustofa The potency of actinomycetes extracts isolated from Pramuka Island, Jakarta, Indonesia as antimicrobial agents
description	Abstract. Setiawati S, Nuryastuti T, Sholikhak EN, Lisdiyanti P, Pratiwi SUT, Sulistiyanti TR, Ratnakomala S, Jumina, Mustofa. 2021. The potency of actinomycetes extracts isolated from Pramuka Island, Jakarta, Indonesia as antimicrobial agents. Biodiversitas 22: 1104-1111. Actinomycetes are one of the Gram-positive bacteria which are widely distributed and produce many secondary metabolites including those known as antibiotics, antifungals, anticancer, and antimalarials agents. The secondary metabolites of actinomycetes are abundant, which include many active compounds that have been identified because of the large diversity in the actinomycetes phylum. This study aimed to identify and screen collected by Indonesian Culture Collection (InaCC) from Bojong Gede and Pramuka Island, Jakarta, Indonesia as antibacterial and antifungal agents. Primary screening was done on 16 actinomycetes isolates by well-agar diffusion method. Antimicrobial activity was tested by using micro broth dilution methods to determine minimum inhibitory concentration (MIC). Molecular identification into level genera and species was determined by 16S rRNA gene sequencing. Out of 16 actinomycetes isolates used, 4 isolates have activity against Candida albicans ATCC 10231, Staphylococcus aureus ATCC 6538, Bacillus subtilis BTCC B-612, and Escherichia coli BTCC B-614, specifically InaCC A758, InaCC A759, InaCC A760 and InaCC A765 isolates. InaCC A758 have highest antimicrobial activity against mentioned microbial with MIC value at 50 ?g/mL, 6.25 ?g/mL, 31.25 ?g/mL and 3.125 ?g/mL, respectively. Three genera were found from the samples: i.e. Streptomyces (80%), Microbispora (13%) and Nocardia (6%). Based on 16S rRNA gene identification, the active isolates of actinomycetes InaCC A758, InaCC A759, InaCC A760 and InaCC A765 were similar to Streptomyces badius, Streptomyces olivaceus, Streptomyces sanyensis, and Nocardia otitidiscaviarum, respectively. The secondary metabolites of actinomycetes extracts from Pramuka Island can be potentially developed as antifungal and antibacterial agents.
format	article
author	Setiawati Setiawati Titik Nuryastuti Eti Nurwening Sholikhah Puspita Lisdiyanti Sylvia Utami Tunjung Pratiwi TRI RATNA SULISTIYANI SHANTI RATNAKOMALA Jumina Jumina Mustofa Mustofa
author_facet	Setiawati Setiawati Titik Nuryastuti Eti Nurwening Sholikhah Puspita Lisdiyanti Sylvia Utami Tunjung Pratiwi TRI RATNA SULISTIYANI SHANTI RATNAKOMALA Jumina Jumina Mustofa Mustofa
author_sort	Setiawati Setiawati
title	The potency of actinomycetes extracts isolated from Pramuka Island, Jakarta, Indonesia as antimicrobial agents
title_short	The potency of actinomycetes extracts isolated from Pramuka Island, Jakarta, Indonesia as antimicrobial agents
title_full	The potency of actinomycetes extracts isolated from Pramuka Island, Jakarta, Indonesia as antimicrobial agents
title_fullStr	The potency of actinomycetes extracts isolated from Pramuka Island, Jakarta, Indonesia as antimicrobial agents
title_full_unstemmed	The potency of actinomycetes extracts isolated from Pramuka Island, Jakarta, Indonesia as antimicrobial agents
title_sort	potency of actinomycetes extracts isolated from pramuka island, jakarta, indonesia as antimicrobial agents
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/c39201284ce4460cbd97b592a022f1ea
work_keys_str_mv	AT setiawatisetiawati thepotencyofactinomycetesextractsisolatedfrompramukaislandjakartaindonesiaasantimicrobialagents AT titiknuryastuti thepotencyofactinomycetesextractsisolatedfrompramukaislandjakartaindonesiaasantimicrobialagents AT etinurweningsholikhah thepotencyofactinomycetesextractsisolatedfrompramukaislandjakartaindonesiaasantimicrobialagents AT puspitalisdiyanti thepotencyofactinomycetesextractsisolatedfrompramukaislandjakartaindonesiaasantimicrobialagents AT sylviautamitunjungpratiwi thepotencyofactinomycetesextractsisolatedfrompramukaislandjakartaindonesiaasantimicrobialagents AT triratnasulistiyani thepotencyofactinomycetesextractsisolatedfrompramukaislandjakartaindonesiaasantimicrobialagents AT shantiratnakomala thepotencyofactinomycetesextractsisolatedfrompramukaislandjakartaindonesiaasantimicrobialagents AT juminajumina thepotencyofactinomycetesextractsisolatedfrompramukaislandjakartaindonesiaasantimicrobialagents AT mustofamustofa thepotencyofactinomycetesextractsisolatedfrompramukaislandjakartaindonesiaasantimicrobialagents AT setiawatisetiawati potencyofactinomycetesextractsisolatedfrompramukaislandjakartaindonesiaasantimicrobialagents AT titiknuryastuti potencyofactinomycetesextractsisolatedfrompramukaislandjakartaindonesiaasantimicrobialagents AT etinurweningsholikhah potencyofactinomycetesextractsisolatedfrompramukaislandjakartaindonesiaasantimicrobialagents AT puspitalisdiyanti potencyofactinomycetesextractsisolatedfrompramukaislandjakartaindonesiaasantimicrobialagents AT sylviautamitunjungpratiwi potencyofactinomycetesextractsisolatedfrompramukaislandjakartaindonesiaasantimicrobialagents AT triratnasulistiyani potencyofactinomycetesextractsisolatedfrompramukaislandjakartaindonesiaasantimicrobialagents AT shantiratnakomala potencyofactinomycetesextractsisolatedfrompramukaislandjakartaindonesiaasantimicrobialagents AT juminajumina potencyofactinomycetesextractsisolatedfrompramukaislandjakartaindonesiaasantimicrobialagents AT mustofamustofa potencyofactinomycetesextractsisolatedfrompramukaislandjakartaindonesiaasantimicrobialagents
_version_	1718418424803098624

The potency of actinomycetes extracts isolated from Pramuka Island, Jakarta, Indonesia as antimicrobial agents

Ejemplares similares