Biostimulatory Potential of Microorganisms from Rosemary (Rosmarinus officinalis L.) Rhizospheric Soil

The objective of the present paper was to isolate microorganisms (Pseudomonas sp., Bacillus sp. and Azotobacter sp.) from the rhizospheric soil of rosemary (Rosmarinus officinalis L.) and investigate their biostimulatory (plant growth-promoting – PGP) and biocontrol potential. The bacteria isolated...

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Autores principales:	Stamenov Dragana, Đurić Simonida, Jafari Timea Hajnal
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2021
Materias:	medicinal plants plant-growth promoting rhizobacteria siderophore iaa Agriculture S
Acceso en línea:	https://doaj.org/article/c39ad2f4d029449c91273c5b6d6c950f
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spelling	oai:doaj.org-article:c39ad2f4d029449c91273c5b6d6c950f2021-12-05T14:11:06ZBiostimulatory Potential of Microorganisms from Rosemary (Rosmarinus officinalis L.) Rhizospheric Soil2466-477410.2478/contagri-2021-0016https://doaj.org/article/c39ad2f4d029449c91273c5b6d6c950f2021-12-01T00:00:00Zhttps://doi.org/10.2478/contagri-2021-0016https://doaj.org/toc/2466-4774The objective of the present paper was to isolate microorganisms (Pseudomonas sp., Bacillus sp. and Azotobacter sp.) from the rhizospheric soil of rosemary (Rosmarinus officinalis L.) and investigate their biostimulatory (plant growth-promoting – PGP) and biocontrol potential. The bacteria isolated from the rhizosphere of rosemary included 15 bacteria of the genus Pseudomonas, 20 of the genus Bacillus, and 11 of the genus Azotobacter. Based on the morphological characteristics of colonies and cells, representative isolates of each genus were chosen (marked as Pseudomonas sp. P42, P43, P44; for Bacillus isolates B83, B84, B85, B92, B93; and for Azotobacter isolates A15 and A16) for different physiological and biochemical examination. The study included in vitro screening of the bacterial isolates for their PGP and biocontrol properties. Pseudomonas isolates showed the ability to live at low temperature (10ºC) and high pH (9), and to use different sources of carbon. All Pseudomonas isolates produced lipase, siderophore, hydrogen cyanide, and utilized organic and inorganic phosphorus, while only isolate P42 produced amylase, pectinase and cellulase. Only Bacillus isolates could grow at 45 ºC (all Bacillus isolates), pH 5 (isolates B83), and on a medium containing NaCl 5 and 7% (all isolates). Isolates denoted as B83 and B93 produced lipase, amylase, and pectinase. All isolates had the ability to solubilize phosphate, produce siderophores (except B85) and hydrogen cyanide, while only two isolates (B84 and B85) produced IAA. Azotobacter isolates had the optimal growth at 37ºC and minimal growth on a medium with pH 6 and 9. All Azotobacter isolates used all carbohydrates as a source of carbon and produced lipase, amylase, and hydrogen cyanide. The best result in suppressing the growth of pathogenic fungi Fusarium oxysporum was achieved by using isolate B92. Application of isolate B83 led to the greatest growth suppression of Sclerotinium sclerotiorum.Stamenov DraganaĐurić SimonidaJafari Timea HajnalSciendoarticlemedicinal plantsplant-growth promoting rhizobacteriasiderophoreiaaAgricultureSENContemporary Agriculture, Vol 70, Iss 3-4, Pp 108-115 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	medicinal plants plant-growth promoting rhizobacteria siderophore iaa Agriculture S
spellingShingle	medicinal plants plant-growth promoting rhizobacteria siderophore iaa Agriculture S Stamenov Dragana Đurić Simonida Jafari Timea Hajnal Biostimulatory Potential of Microorganisms from Rosemary (Rosmarinus officinalis L.) Rhizospheric Soil
description	The objective of the present paper was to isolate microorganisms (Pseudomonas sp., Bacillus sp. and Azotobacter sp.) from the rhizospheric soil of rosemary (Rosmarinus officinalis L.) and investigate their biostimulatory (plant growth-promoting – PGP) and biocontrol potential. The bacteria isolated from the rhizosphere of rosemary included 15 bacteria of the genus Pseudomonas, 20 of the genus Bacillus, and 11 of the genus Azotobacter. Based on the morphological characteristics of colonies and cells, representative isolates of each genus were chosen (marked as Pseudomonas sp. P42, P43, P44; for Bacillus isolates B83, B84, B85, B92, B93; and for Azotobacter isolates A15 and A16) for different physiological and biochemical examination. The study included in vitro screening of the bacterial isolates for their PGP and biocontrol properties. Pseudomonas isolates showed the ability to live at low temperature (10ºC) and high pH (9), and to use different sources of carbon. All Pseudomonas isolates produced lipase, siderophore, hydrogen cyanide, and utilized organic and inorganic phosphorus, while only isolate P42 produced amylase, pectinase and cellulase. Only Bacillus isolates could grow at 45 ºC (all Bacillus isolates), pH 5 (isolates B83), and on a medium containing NaCl 5 and 7% (all isolates). Isolates denoted as B83 and B93 produced lipase, amylase, and pectinase. All isolates had the ability to solubilize phosphate, produce siderophores (except B85) and hydrogen cyanide, while only two isolates (B84 and B85) produced IAA. Azotobacter isolates had the optimal growth at 37ºC and minimal growth on a medium with pH 6 and 9. All Azotobacter isolates used all carbohydrates as a source of carbon and produced lipase, amylase, and hydrogen cyanide. The best result in suppressing the growth of pathogenic fungi Fusarium oxysporum was achieved by using isolate B92. Application of isolate B83 led to the greatest growth suppression of Sclerotinium sclerotiorum.
format	article
author	Stamenov Dragana Đurić Simonida Jafari Timea Hajnal
author_facet	Stamenov Dragana Đurić Simonida Jafari Timea Hajnal
author_sort	Stamenov Dragana
title	Biostimulatory Potential of Microorganisms from Rosemary (Rosmarinus officinalis L.) Rhizospheric Soil
title_short	Biostimulatory Potential of Microorganisms from Rosemary (Rosmarinus officinalis L.) Rhizospheric Soil
title_full	Biostimulatory Potential of Microorganisms from Rosemary (Rosmarinus officinalis L.) Rhizospheric Soil
title_fullStr	Biostimulatory Potential of Microorganisms from Rosemary (Rosmarinus officinalis L.) Rhizospheric Soil
title_full_unstemmed	Biostimulatory Potential of Microorganisms from Rosemary (Rosmarinus officinalis L.) Rhizospheric Soil
title_sort	biostimulatory potential of microorganisms from rosemary (rosmarinus officinalis l.) rhizospheric soil
publisher	Sciendo
publishDate	2021
url	https://doaj.org/article/c39ad2f4d029449c91273c5b6d6c950f
work_keys_str_mv	AT stamenovdragana biostimulatorypotentialofmicroorganismsfromrosemaryrosmarinusofficinalislrhizosphericsoil AT đuricsimonida biostimulatorypotentialofmicroorganismsfromrosemaryrosmarinusofficinalislrhizosphericsoil AT jafaritimeahajnal biostimulatorypotentialofmicroorganismsfromrosemaryrosmarinusofficinalislrhizosphericsoil
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Biostimulatory Potential of Microorganisms from Rosemary (Rosmarinus officinalis L.) Rhizospheric Soil

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