Prevalence of diarrhoeal pathogens among children under five years of age with and without diarrhoea in Guinea-Bissau.

<h4>Background</h4>Childhood diarrhoea, a major cause of morbidity and mortality in low-income regions, remains scarcely studied in many countries, such as Guinea-Bissau. Stool sample drying enables later qPCR analyses of pathogens without concern about electricity shortages.<h4>Me...

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Autores principales:	Sointu Mero, Suvi Timonen, Tinja Lääveri, Sandra Løfberg, Juha Kirveskari, Johan Ursing, Lars Rombo, Poul-Erik Kofoed, Anu Kantele
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/c3a52d288cb4492b99babd02e8a60a6c
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spelling	oai:doaj.org-article:c3a52d288cb4492b99babd02e8a60a6c2021-12-02T20:24:01ZPrevalence of diarrhoeal pathogens among children under five years of age with and without diarrhoea in Guinea-Bissau.1935-27271935-273510.1371/journal.pntd.0009709https://doaj.org/article/c3a52d288cb4492b99babd02e8a60a6c2021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009709https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735<h4>Background</h4>Childhood diarrhoea, a major cause of morbidity and mortality in low-income regions, remains scarcely studied in many countries, such as Guinea-Bissau. Stool sample drying enables later qPCR analyses of pathogens without concern about electricity shortages.<h4>Methods</h4>Dried stool samples of children under five years treated at the Bandim Health Centre in Bissau, Guinea-Bissau were screened by qPCR for nine enteric bacteria, five viruses, and four parasites. The findings of children having and not having diarrhoea were compared in age groups 0-11 and 12-59 months.<h4>Results</h4>Of the 429 children- 228 with and 201 without diarrhoea- 96.9% and 93.5% had bacterial, 62.7% and 44.3% viral, and 52.6% and 48.3% parasitic pathogen findings, respectively. Enteroaggregarive Escherichia coli (EAEC; 60.5% versus 66.7%), enteropathogenic E. coli (EPEC; 61.4% versus 62.7%), Campylobacter (53.2% versus 51.8%), and enterotoxigenic E. coli (ETEC; 54.4% versus 44.3%) were the most common bacterial pathogens. Diarrhoea was associated with enteroinvasive E. coli (EIEC)/Shigella (63.3%), ETEC (54.4%), astrovirus (75.0%), norovirus GII (72.6%) and Cryptosporidium (71.2%). The only pathogen associated with severe diarrhoea was EIEC/Shigella (p<0.001). EAEC was found more frequent among the infants, and EIEC/Shigella, Giardia duodenalis and Dientamoeba fragilis among the older children.<h4>Conclusions</h4>Stool pathogens proved common among all the children regardless of them having diarrhoea or not.Sointu MeroSuvi TimonenTinja LääveriSandra LøfbergJuha KirveskariJohan UrsingLars RomboPoul-Erik KofoedAnu KantelePublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 9, p e0009709 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Sointu Mero Suvi Timonen Tinja Lääveri Sandra Løfberg Juha Kirveskari Johan Ursing Lars Rombo Poul-Erik Kofoed Anu Kantele Prevalence of diarrhoeal pathogens among children under five years of age with and without diarrhoea in Guinea-Bissau.
description	<h4>Background</h4>Childhood diarrhoea, a major cause of morbidity and mortality in low-income regions, remains scarcely studied in many countries, such as Guinea-Bissau. Stool sample drying enables later qPCR analyses of pathogens without concern about electricity shortages.<h4>Methods</h4>Dried stool samples of children under five years treated at the Bandim Health Centre in Bissau, Guinea-Bissau were screened by qPCR for nine enteric bacteria, five viruses, and four parasites. The findings of children having and not having diarrhoea were compared in age groups 0-11 and 12-59 months.<h4>Results</h4>Of the 429 children- 228 with and 201 without diarrhoea- 96.9% and 93.5% had bacterial, 62.7% and 44.3% viral, and 52.6% and 48.3% parasitic pathogen findings, respectively. Enteroaggregarive Escherichia coli (EAEC; 60.5% versus 66.7%), enteropathogenic E. coli (EPEC; 61.4% versus 62.7%), Campylobacter (53.2% versus 51.8%), and enterotoxigenic E. coli (ETEC; 54.4% versus 44.3%) were the most common bacterial pathogens. Diarrhoea was associated with enteroinvasive E. coli (EIEC)/Shigella (63.3%), ETEC (54.4%), astrovirus (75.0%), norovirus GII (72.6%) and Cryptosporidium (71.2%). The only pathogen associated with severe diarrhoea was EIEC/Shigella (p<0.001). EAEC was found more frequent among the infants, and EIEC/Shigella, Giardia duodenalis and Dientamoeba fragilis among the older children.<h4>Conclusions</h4>Stool pathogens proved common among all the children regardless of them having diarrhoea or not.
format	article
author	Sointu Mero Suvi Timonen Tinja Lääveri Sandra Løfberg Juha Kirveskari Johan Ursing Lars Rombo Poul-Erik Kofoed Anu Kantele
author_facet	Sointu Mero Suvi Timonen Tinja Lääveri Sandra Løfberg Juha Kirveskari Johan Ursing Lars Rombo Poul-Erik Kofoed Anu Kantele
author_sort	Sointu Mero
title	Prevalence of diarrhoeal pathogens among children under five years of age with and without diarrhoea in Guinea-Bissau.
title_short	Prevalence of diarrhoeal pathogens among children under five years of age with and without diarrhoea in Guinea-Bissau.
title_full	Prevalence of diarrhoeal pathogens among children under five years of age with and without diarrhoea in Guinea-Bissau.
title_fullStr	Prevalence of diarrhoeal pathogens among children under five years of age with and without diarrhoea in Guinea-Bissau.
title_full_unstemmed	Prevalence of diarrhoeal pathogens among children under five years of age with and without diarrhoea in Guinea-Bissau.
title_sort	prevalence of diarrhoeal pathogens among children under five years of age with and without diarrhoea in guinea-bissau.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/c3a52d288cb4492b99babd02e8a60a6c
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Prevalence of diarrhoeal pathogens among children under five years of age with and without diarrhoea in Guinea-Bissau.

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