Chick Embryo Experimental Platform for Micrometastases Research in a 3D Tissue Engineering Model: Cancer Biology, Drug Development, and Nanotechnology Applications

Colonization of distant organs by tumor cells is a critical step of cancer progression. The initial avascular stage of this process (micrometastasis) remains almost inaccessible to study due to the lack of relevant experimental approaches. Herein, we introduce an in vitro/in vivo model of organ-spec...

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Autores principales:	Anna Guller, Inga Kuschnerus, Vlada Rozova, Annemarie Nadort, Yin Yao, Zahra Khabir, Alfonso Garcia-Bennett, Liuen (Olivia) Liang, Aleksandra Polikarpova, Yi Qian, Ewa M. Goldys, Andrei V. Zvyagin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	chick embryo 3D culture tumor models in vitro tissue engineering metastasis micrometastasis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c3a6cbfc9d7e43ea87d9f86ad8196f2b
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spelling	oai:doaj.org-article:c3a6cbfc9d7e43ea87d9f86ad8196f2b2021-11-25T16:49:13ZChick Embryo Experimental Platform for Micrometastases Research in a 3D Tissue Engineering Model: Cancer Biology, Drug Development, and Nanotechnology Applications10.3390/biomedicines91115782227-9059https://doaj.org/article/c3a6cbfc9d7e43ea87d9f86ad8196f2b2021-10-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1578https://doaj.org/toc/2227-9059Colonization of distant organs by tumor cells is a critical step of cancer progression. The initial avascular stage of this process (micrometastasis) remains almost inaccessible to study due to the lack of relevant experimental approaches. Herein, we introduce an in vitro/in vivo model of organ-specific micrometastases of triple-negative breast cancer (TNBC) that is fully implemented in a cost-efficient chick embryo (CE) experimental platform. The model was built as three-dimensional (3D) tissue engineering constructs (TECs) combining human MDA-MB-231 cells and decellularized CE organ-specific scaffolds. TNBC cells colonized CE organ-specific scaffolds in 2–3 weeks, forming tissue-like structures. The feasibility of this methodology for basic cancer research, drug development, and nanomedicine was demonstrated on a model of hepatic micrometastasis of TNBC. We revealed that MDA-MB-231 differentially colonize parenchymal and stromal compartments of the liver-specific extracellular matrix (LS-ECM) and become more resistant to the treatment with molecular doxorubicin (Dox) and Dox-loaded mesoporous silica nanoparticles than in monolayer cultures. When grafted on CE chorioallantoic membrane, LS-ECM-based TECs induced angiogenic switch. These findings may have important implications for the diagnosis and treatment of TNBC. The methodology established here is scalable and adaptable for pharmacological testing and cancer biology research of various metastatic and primary tumors.Anna GullerInga KuschnerusVlada RozovaAnnemarie NadortYin YaoZahra KhabirAlfonso Garcia-BennettLiuen (Olivia) LiangAleksandra PolikarpovaYi QianEwa M. GoldysAndrei V. ZvyaginMDPI AGarticlechick embryo3D culturetumor models in vitrotissue engineeringmetastasismicrometastasisBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1578, p 1578 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chick embryo 3D culture tumor models in vitro tissue engineering metastasis micrometastasis Biology (General) QH301-705.5
spellingShingle	chick embryo 3D culture tumor models in vitro tissue engineering metastasis micrometastasis Biology (General) QH301-705.5 Anna Guller Inga Kuschnerus Vlada Rozova Annemarie Nadort Yin Yao Zahra Khabir Alfonso Garcia-Bennett Liuen (Olivia) Liang Aleksandra Polikarpova Yi Qian Ewa M. Goldys Andrei V. Zvyagin Chick Embryo Experimental Platform for Micrometastases Research in a 3D Tissue Engineering Model: Cancer Biology, Drug Development, and Nanotechnology Applications
description	Colonization of distant organs by tumor cells is a critical step of cancer progression. The initial avascular stage of this process (micrometastasis) remains almost inaccessible to study due to the lack of relevant experimental approaches. Herein, we introduce an in vitro/in vivo model of organ-specific micrometastases of triple-negative breast cancer (TNBC) that is fully implemented in a cost-efficient chick embryo (CE) experimental platform. The model was built as three-dimensional (3D) tissue engineering constructs (TECs) combining human MDA-MB-231 cells and decellularized CE organ-specific scaffolds. TNBC cells colonized CE organ-specific scaffolds in 2–3 weeks, forming tissue-like structures. The feasibility of this methodology for basic cancer research, drug development, and nanomedicine was demonstrated on a model of hepatic micrometastasis of TNBC. We revealed that MDA-MB-231 differentially colonize parenchymal and stromal compartments of the liver-specific extracellular matrix (LS-ECM) and become more resistant to the treatment with molecular doxorubicin (Dox) and Dox-loaded mesoporous silica nanoparticles than in monolayer cultures. When grafted on CE chorioallantoic membrane, LS-ECM-based TECs induced angiogenic switch. These findings may have important implications for the diagnosis and treatment of TNBC. The methodology established here is scalable and adaptable for pharmacological testing and cancer biology research of various metastatic and primary tumors.
format	article
author	Anna Guller Inga Kuschnerus Vlada Rozova Annemarie Nadort Yin Yao Zahra Khabir Alfonso Garcia-Bennett Liuen (Olivia) Liang Aleksandra Polikarpova Yi Qian Ewa M. Goldys Andrei V. Zvyagin
author_facet	Anna Guller Inga Kuschnerus Vlada Rozova Annemarie Nadort Yin Yao Zahra Khabir Alfonso Garcia-Bennett Liuen (Olivia) Liang Aleksandra Polikarpova Yi Qian Ewa M. Goldys Andrei V. Zvyagin
author_sort	Anna Guller
title	Chick Embryo Experimental Platform for Micrometastases Research in a 3D Tissue Engineering Model: Cancer Biology, Drug Development, and Nanotechnology Applications
title_short	Chick Embryo Experimental Platform for Micrometastases Research in a 3D Tissue Engineering Model: Cancer Biology, Drug Development, and Nanotechnology Applications
title_full	Chick Embryo Experimental Platform for Micrometastases Research in a 3D Tissue Engineering Model: Cancer Biology, Drug Development, and Nanotechnology Applications
title_fullStr	Chick Embryo Experimental Platform for Micrometastases Research in a 3D Tissue Engineering Model: Cancer Biology, Drug Development, and Nanotechnology Applications
title_full_unstemmed	Chick Embryo Experimental Platform for Micrometastases Research in a 3D Tissue Engineering Model: Cancer Biology, Drug Development, and Nanotechnology Applications
title_sort	chick embryo experimental platform for micrometastases research in a 3d tissue engineering model: cancer biology, drug development, and nanotechnology applications
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c3a6cbfc9d7e43ea87d9f86ad8196f2b
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Chick Embryo Experimental Platform for Micrometastases Research in a 3D Tissue Engineering Model: Cancer Biology, Drug Development, and Nanotechnology Applications

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