Olig2 regulates p53-mediated apoptosis, migration and invasion of melanoma cells

Abstract Melanoma is a disease with a high recurrence rate and poor prognosis; therefore, the need for targeted therapeutics is steadily increasing. Oligodendrocyte transcription factor2 (Olig2) is a basic helix-loop-helix transcription factor that is expressed in the central nervous system during e...

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Autores principales:	Ji Eun Lee, Sungjin Ahn, Haengdueng Jeong, Seungchan An, Cheol Hwan Myung, Jeong Ah Lee, Sung Chan Hong, Youn Jin Kim, Jin Young Kim, Jong Hyuk Ryu, Minsoo Noh, Ki Taek Nam, Jae Sung Hwang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c3a8b226e3e04cfe97a00e1601878eed
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spelling	oai:doaj.org-article:c3a8b226e3e04cfe97a00e1601878eed2021-12-02T14:26:51ZOlig2 regulates p53-mediated apoptosis, migration and invasion of melanoma cells10.1038/s41598-021-87438-x2045-2322https://doaj.org/article/c3a8b226e3e04cfe97a00e1601878eed2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87438-xhttps://doaj.org/toc/2045-2322Abstract Melanoma is a disease with a high recurrence rate and poor prognosis; therefore, the need for targeted therapeutics is steadily increasing. Oligodendrocyte transcription factor2 (Olig2) is a basic helix-loop-helix transcription factor that is expressed in the central nervous system during embryonic development. Olig2 is overexpressed in various malignant cell lines such as lung carcinoma, glioma and melanoma. Olig2 is known as a key transcription factor that promotes tumor growth in malignant glioma. However, the role of Olig2 in melanoma is not well characterized. We analyzed the role of Olig2 in apoptosis, migration, and invasion of melanoma cells. We confirmed that Olig2 was overexpressed in melanoma cells and tissues. Reduction of Olig2 increased apoptosis in melanoma cells by increasing p53 level and caspase-3/-7 enzyme activity. In addition, downregulation of Olig2 suppressed migration and invasion of melanoma cells by inhibiting EMT. Reduction of Olig2 inhibited expression of MMP-1 and the enzyme activity of MMP-2/-9 induced by TGF-β. Moreover, Olig2 was involved in the downstream stages of MEK/ERK and PI3K/AKT, which are major signaling pathways in metastatic progression of melanoma. In conclusion, this study demonstrated the crucial roles of Olig2 in apoptosis, migration, and invasion of melanoma and may help to further our understanding of the relationship between Olig2 and melanoma progression.Ji Eun LeeSungjin AhnHaengdueng JeongSeungchan AnCheol Hwan MyungJeong Ah LeeSung Chan HongYoun Jin KimJin Young KimJong Hyuk RyuMinsoo NohKi Taek NamJae Sung HwangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ji Eun Lee Sungjin Ahn Haengdueng Jeong Seungchan An Cheol Hwan Myung Jeong Ah Lee Sung Chan Hong Youn Jin Kim Jin Young Kim Jong Hyuk Ryu Minsoo Noh Ki Taek Nam Jae Sung Hwang Olig2 regulates p53-mediated apoptosis, migration and invasion of melanoma cells
description	Abstract Melanoma is a disease with a high recurrence rate and poor prognosis; therefore, the need for targeted therapeutics is steadily increasing. Oligodendrocyte transcription factor2 (Olig2) is a basic helix-loop-helix transcription factor that is expressed in the central nervous system during embryonic development. Olig2 is overexpressed in various malignant cell lines such as lung carcinoma, glioma and melanoma. Olig2 is known as a key transcription factor that promotes tumor growth in malignant glioma. However, the role of Olig2 in melanoma is not well characterized. We analyzed the role of Olig2 in apoptosis, migration, and invasion of melanoma cells. We confirmed that Olig2 was overexpressed in melanoma cells and tissues. Reduction of Olig2 increased apoptosis in melanoma cells by increasing p53 level and caspase-3/-7 enzyme activity. In addition, downregulation of Olig2 suppressed migration and invasion of melanoma cells by inhibiting EMT. Reduction of Olig2 inhibited expression of MMP-1 and the enzyme activity of MMP-2/-9 induced by TGF-β. Moreover, Olig2 was involved in the downstream stages of MEK/ERK and PI3K/AKT, which are major signaling pathways in metastatic progression of melanoma. In conclusion, this study demonstrated the crucial roles of Olig2 in apoptosis, migration, and invasion of melanoma and may help to further our understanding of the relationship between Olig2 and melanoma progression.
format	article
author	Ji Eun Lee Sungjin Ahn Haengdueng Jeong Seungchan An Cheol Hwan Myung Jeong Ah Lee Sung Chan Hong Youn Jin Kim Jin Young Kim Jong Hyuk Ryu Minsoo Noh Ki Taek Nam Jae Sung Hwang
author_facet	Ji Eun Lee Sungjin Ahn Haengdueng Jeong Seungchan An Cheol Hwan Myung Jeong Ah Lee Sung Chan Hong Youn Jin Kim Jin Young Kim Jong Hyuk Ryu Minsoo Noh Ki Taek Nam Jae Sung Hwang
author_sort	Ji Eun Lee
title	Olig2 regulates p53-mediated apoptosis, migration and invasion of melanoma cells
title_short	Olig2 regulates p53-mediated apoptosis, migration and invasion of melanoma cells
title_full	Olig2 regulates p53-mediated apoptosis, migration and invasion of melanoma cells
title_fullStr	Olig2 regulates p53-mediated apoptosis, migration and invasion of melanoma cells
title_full_unstemmed	Olig2 regulates p53-mediated apoptosis, migration and invasion of melanoma cells
title_sort	olig2 regulates p53-mediated apoptosis, migration and invasion of melanoma cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c3a8b226e3e04cfe97a00e1601878eed
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Olig2 regulates p53-mediated apoptosis, migration and invasion of melanoma cells

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