iNOS promotes hypothalamic insulin resistance associated with deregulation of energy balance and obesity in rodents

Abstract Inducible nitric oxide (iNOS)-mediated S-nitrosation of the metabolic signaling pathway has emerged as a post-translational modification that triggers insulin resistance in obesity and aging. However, the effects of S-nitrosation in controlling energy homeostasis are unknown. Thus, in the p...

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Autores principales:	Carlos Kiyoshi Katashima, Vagner Ramon Rodrigues Silva, Luciene Lenhare, Rodrigo Miguel Marin, José Barreto Campello Carvalheira
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c3aa083aeca14bd2aebfe2399c96cba1
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spelling	oai:doaj.org-article:c3aa083aeca14bd2aebfe2399c96cba12021-12-02T11:52:21ZiNOS promotes hypothalamic insulin resistance associated with deregulation of energy balance and obesity in rodents10.1038/s41598-017-08920-z2045-2322https://doaj.org/article/c3aa083aeca14bd2aebfe2399c96cba12017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08920-zhttps://doaj.org/toc/2045-2322Abstract Inducible nitric oxide (iNOS)-mediated S-nitrosation of the metabolic signaling pathway has emerged as a post-translational modification that triggers insulin resistance in obesity and aging. However, the effects of S-nitrosation in controlling energy homeostasis are unknown. Thus, in the present study we aimed to evaluate the effects of S-nitrosation in insulin signaling pathway in the hypothalamus of rodents. Herein, we demonstrated that the intracerebroventricular infusion of the nitric oxide (NO) donor S-nitrosoglutathione (GSNO) promoted hypothalamic insulin signaling resistance and replicated the food intake pattern of obese individuals. Indeed, obesity induced S-nitrosation of hypothalamic IR and Akt, whereas inhibition of iNOS or S-nitrosation of insulin signaling pathway protected against hypothalamic insulin resistance and normalized energy homeostasis. Overall, these findings indicated that S-nitrosation of insulin signaling pathway is required to sustain hypothalamic insulin resistance in obesity.Carlos Kiyoshi KatashimaVagner Ramon Rodrigues SilvaLuciene LenhareRodrigo Miguel MarinJosé Barreto Campello CarvalheiraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carlos Kiyoshi Katashima Vagner Ramon Rodrigues Silva Luciene Lenhare Rodrigo Miguel Marin José Barreto Campello Carvalheira iNOS promotes hypothalamic insulin resistance associated with deregulation of energy balance and obesity in rodents
description	Abstract Inducible nitric oxide (iNOS)-mediated S-nitrosation of the metabolic signaling pathway has emerged as a post-translational modification that triggers insulin resistance in obesity and aging. However, the effects of S-nitrosation in controlling energy homeostasis are unknown. Thus, in the present study we aimed to evaluate the effects of S-nitrosation in insulin signaling pathway in the hypothalamus of rodents. Herein, we demonstrated that the intracerebroventricular infusion of the nitric oxide (NO) donor S-nitrosoglutathione (GSNO) promoted hypothalamic insulin signaling resistance and replicated the food intake pattern of obese individuals. Indeed, obesity induced S-nitrosation of hypothalamic IR and Akt, whereas inhibition of iNOS or S-nitrosation of insulin signaling pathway protected against hypothalamic insulin resistance and normalized energy homeostasis. Overall, these findings indicated that S-nitrosation of insulin signaling pathway is required to sustain hypothalamic insulin resistance in obesity.
format	article
author	Carlos Kiyoshi Katashima Vagner Ramon Rodrigues Silva Luciene Lenhare Rodrigo Miguel Marin José Barreto Campello Carvalheira
author_facet	Carlos Kiyoshi Katashima Vagner Ramon Rodrigues Silva Luciene Lenhare Rodrigo Miguel Marin José Barreto Campello Carvalheira
author_sort	Carlos Kiyoshi Katashima
title	iNOS promotes hypothalamic insulin resistance associated with deregulation of energy balance and obesity in rodents
title_short	iNOS promotes hypothalamic insulin resistance associated with deregulation of energy balance and obesity in rodents
title_full	iNOS promotes hypothalamic insulin resistance associated with deregulation of energy balance and obesity in rodents
title_fullStr	iNOS promotes hypothalamic insulin resistance associated with deregulation of energy balance and obesity in rodents
title_full_unstemmed	iNOS promotes hypothalamic insulin resistance associated with deregulation of energy balance and obesity in rodents
title_sort	inos promotes hypothalamic insulin resistance associated with deregulation of energy balance and obesity in rodents
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c3aa083aeca14bd2aebfe2399c96cba1
work_keys_str_mv	AT carloskiyoshikatashima inospromoteshypothalamicinsulinresistanceassociatedwithderegulationofenergybalanceandobesityinrodents AT vagnerramonrodriguessilva inospromoteshypothalamicinsulinresistanceassociatedwithderegulationofenergybalanceandobesityinrodents AT lucienelenhare inospromoteshypothalamicinsulinresistanceassociatedwithderegulationofenergybalanceandobesityinrodents AT rodrigomiguelmarin inospromoteshypothalamicinsulinresistanceassociatedwithderegulationofenergybalanceandobesityinrodents AT josebarretocampellocarvalheira inospromoteshypothalamicinsulinresistanceassociatedwithderegulationofenergybalanceandobesityinrodents
_version_	1718395068316909568

iNOS promotes hypothalamic insulin resistance associated with deregulation of energy balance and obesity in rodents

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