Inhibitory effect of metformin and pyridoxamine in the formation of early, intermediate and advanced glycation end-products.

<h4>Background</h4>Non-enzymatic glycation is the addition of free carbonyl group of reducing sugar to the free amino groups of proteins, resulting in the formation of a Schiff base and an Amadori product. Dihydroxyacetone (DHA) is one of the carbonyl species which reacts rapidly with th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Saheem Ahmad, Uzma Shahab, Mohd Hassan Baig, Mohd Sajid Khan, M Salman Khan, A K Srivastava, Mohd Saeed, Moinuddin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c3afbd706c0b418d8e83c3b32f50f7b4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c3afbd706c0b418d8e83c3b32f50f7b4
record_format	dspace
spelling	oai:doaj.org-article:c3afbd706c0b418d8e83c3b32f50f7b42021-11-18T08:57:05ZInhibitory effect of metformin and pyridoxamine in the formation of early, intermediate and advanced glycation end-products.1932-620310.1371/journal.pone.0072128https://doaj.org/article/c3afbd706c0b418d8e83c3b32f50f7b42013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24023728/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Non-enzymatic glycation is the addition of free carbonyl group of reducing sugar to the free amino groups of proteins, resulting in the formation of a Schiff base and an Amadori product. Dihydroxyacetone (DHA) is one of the carbonyl species which reacts rapidly with the free amino groups of proteins to form advanced glycation end products (AGEs). The highly reactive dihydroxyacetone phosphate is a derivative of dihydroxyacetone (DHA), and a product of glycolysis, having potential glycating effects to form AGEs. The formation of AGEs results in the generation of free radicals which play an important role in the pathophysiology of aging and diabetic complications. While the formation of DHA-AGEs has been demonstrated previously, no extensive studies have been performed to assess the inhibition of AGE inhibitors at all the three stages of glycation (early, intermediate and late) using metformin (MF) and pyridoxamine (PM) as a novel inhibitor.<h4>Methodology/principal findings</h4>In this study we report glycation of human serum albumin (HSA) & its characterization by various spectroscopic techniques. Furthermore, inhibition of glycation products at all the stages of glycation was also studied. Spectroscopic analysis suggests structural perturbations in the HSA as a result of modification which might be due to generation of free radicals and formation of AGEs.<h4>Conclusion</h4>The inhibition in the formation of glycation reaction reveals that Pyridoxamine is a better antiglycating agent than Metformin at all stages of the glycation (early, intermediate and late stages).Saheem AhmadUzma ShahabMohd Hassan BaigMohd Sajid KhanM Salman KhanA K SrivastavaMohd SaeedMoinuddinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e72128 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Saheem Ahmad Uzma Shahab Mohd Hassan Baig Mohd Sajid Khan M Salman Khan A K Srivastava Mohd Saeed Moinuddin Inhibitory effect of metformin and pyridoxamine in the formation of early, intermediate and advanced glycation end-products.
description	<h4>Background</h4>Non-enzymatic glycation is the addition of free carbonyl group of reducing sugar to the free amino groups of proteins, resulting in the formation of a Schiff base and an Amadori product. Dihydroxyacetone (DHA) is one of the carbonyl species which reacts rapidly with the free amino groups of proteins to form advanced glycation end products (AGEs). The highly reactive dihydroxyacetone phosphate is a derivative of dihydroxyacetone (DHA), and a product of glycolysis, having potential glycating effects to form AGEs. The formation of AGEs results in the generation of free radicals which play an important role in the pathophysiology of aging and diabetic complications. While the formation of DHA-AGEs has been demonstrated previously, no extensive studies have been performed to assess the inhibition of AGE inhibitors at all the three stages of glycation (early, intermediate and late) using metformin (MF) and pyridoxamine (PM) as a novel inhibitor.<h4>Methodology/principal findings</h4>In this study we report glycation of human serum albumin (HSA) & its characterization by various spectroscopic techniques. Furthermore, inhibition of glycation products at all the stages of glycation was also studied. Spectroscopic analysis suggests structural perturbations in the HSA as a result of modification which might be due to generation of free radicals and formation of AGEs.<h4>Conclusion</h4>The inhibition in the formation of glycation reaction reveals that Pyridoxamine is a better antiglycating agent than Metformin at all stages of the glycation (early, intermediate and late stages).
format	article
author	Saheem Ahmad Uzma Shahab Mohd Hassan Baig Mohd Sajid Khan M Salman Khan A K Srivastava Mohd Saeed Moinuddin
author_facet	Saheem Ahmad Uzma Shahab Mohd Hassan Baig Mohd Sajid Khan M Salman Khan A K Srivastava Mohd Saeed Moinuddin
author_sort	Saheem Ahmad
title	Inhibitory effect of metformin and pyridoxamine in the formation of early, intermediate and advanced glycation end-products.
title_short	Inhibitory effect of metformin and pyridoxamine in the formation of early, intermediate and advanced glycation end-products.
title_full	Inhibitory effect of metformin and pyridoxamine in the formation of early, intermediate and advanced glycation end-products.
title_fullStr	Inhibitory effect of metformin and pyridoxamine in the formation of early, intermediate and advanced glycation end-products.
title_full_unstemmed	Inhibitory effect of metformin and pyridoxamine in the formation of early, intermediate and advanced glycation end-products.
title_sort	inhibitory effect of metformin and pyridoxamine in the formation of early, intermediate and advanced glycation end-products.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/c3afbd706c0b418d8e83c3b32f50f7b4
work_keys_str_mv	AT saheemahmad inhibitoryeffectofmetforminandpyridoxamineintheformationofearlyintermediateandadvancedglycationendproducts AT uzmashahab inhibitoryeffectofmetforminandpyridoxamineintheformationofearlyintermediateandadvancedglycationendproducts AT mohdhassanbaig inhibitoryeffectofmetforminandpyridoxamineintheformationofearlyintermediateandadvancedglycationendproducts AT mohdsajidkhan inhibitoryeffectofmetforminandpyridoxamineintheformationofearlyintermediateandadvancedglycationendproducts AT msalmankhan inhibitoryeffectofmetforminandpyridoxamineintheformationofearlyintermediateandadvancedglycationendproducts AT aksrivastava inhibitoryeffectofmetforminandpyridoxamineintheformationofearlyintermediateandadvancedglycationendproducts AT mohdsaeed inhibitoryeffectofmetforminandpyridoxamineintheformationofearlyintermediateandadvancedglycationendproducts AT moinuddin inhibitoryeffectofmetforminandpyridoxamineintheformationofearlyintermediateandadvancedglycationendproducts
_version_	1718421164085215232

Inhibitory effect of metformin and pyridoxamine in the formation of early, intermediate and advanced glycation end-products.

Ejemplares similares