In situ electroporation of mammalian cells through SiO2 thin film capacitive microelectrodes

Abstract Electroporation is a widely used non-viral technique for the delivery of molecules, including nucleic acids, into cells. Recently, electronic microsystems that miniaturize the electroporation machinery have been developed as a new tool for genetic manipulation of cells in vitro, by integrat...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	M. Maschietto, M. Dal Maschio, S. Girardi, S. Vassanelli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c3b8b18974f3433685404d5ef483bb5b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c3b8b18974f3433685404d5ef483bb5b
record_format	dspace
spelling	oai:doaj.org-article:c3b8b18974f3433685404d5ef483bb5b2021-12-02T16:26:38ZIn situ electroporation of mammalian cells through SiO2 thin film capacitive microelectrodes10.1038/s41598-021-94620-82045-2322https://doaj.org/article/c3b8b18974f3433685404d5ef483bb5b2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94620-8https://doaj.org/toc/2045-2322Abstract Electroporation is a widely used non-viral technique for the delivery of molecules, including nucleic acids, into cells. Recently, electronic microsystems that miniaturize the electroporation machinery have been developed as a new tool for genetic manipulation of cells in vitro, by integrating metal microelectrodes in the culture substrate and enabling electroporation in-situ. We report that non-faradic SiO2 thin film-insulated microelectrodes can be used for reliable and spatially selective in-situ electroporation of mammalian cells. CHO-K1 and SH-SY5Y cell lines and primary neuronal cultures were electroporated by application of short and low amplitude voltage transients leading to cell electroporation by capacitive currents. We demonstrate reliable delivery of DNA plasmids and exogenous gene expression, accompanied by high spatial selectivity and cell viability, even with differentiated neurons. Finally, we show that SiO2 thin film-insulated microelectrodes support a double and serial transfection of the targeted cells.M. MaschiettoM. Dal MaschioS. GirardiS. VassanelliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M. Maschietto M. Dal Maschio S. Girardi S. Vassanelli In situ electroporation of mammalian cells through SiO2 thin film capacitive microelectrodes
description	Abstract Electroporation is a widely used non-viral technique for the delivery of molecules, including nucleic acids, into cells. Recently, electronic microsystems that miniaturize the electroporation machinery have been developed as a new tool for genetic manipulation of cells in vitro, by integrating metal microelectrodes in the culture substrate and enabling electroporation in-situ. We report that non-faradic SiO2 thin film-insulated microelectrodes can be used for reliable and spatially selective in-situ electroporation of mammalian cells. CHO-K1 and SH-SY5Y cell lines and primary neuronal cultures were electroporated by application of short and low amplitude voltage transients leading to cell electroporation by capacitive currents. We demonstrate reliable delivery of DNA plasmids and exogenous gene expression, accompanied by high spatial selectivity and cell viability, even with differentiated neurons. Finally, we show that SiO2 thin film-insulated microelectrodes support a double and serial transfection of the targeted cells.
format	article
author	M. Maschietto M. Dal Maschio S. Girardi S. Vassanelli
author_facet	M. Maschietto M. Dal Maschio S. Girardi S. Vassanelli
author_sort	M. Maschietto
title	In situ electroporation of mammalian cells through SiO2 thin film capacitive microelectrodes
title_short	In situ electroporation of mammalian cells through SiO2 thin film capacitive microelectrodes
title_full	In situ electroporation of mammalian cells through SiO2 thin film capacitive microelectrodes
title_fullStr	In situ electroporation of mammalian cells through SiO2 thin film capacitive microelectrodes
title_full_unstemmed	In situ electroporation of mammalian cells through SiO2 thin film capacitive microelectrodes
title_sort	in situ electroporation of mammalian cells through sio2 thin film capacitive microelectrodes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c3b8b18974f3433685404d5ef483bb5b
work_keys_str_mv	AT mmaschietto insituelectroporationofmammaliancellsthroughsio2thinfilmcapacitivemicroelectrodes AT mdalmaschio insituelectroporationofmammaliancellsthroughsio2thinfilmcapacitivemicroelectrodes AT sgirardi insituelectroporationofmammaliancellsthroughsio2thinfilmcapacitivemicroelectrodes AT svassanelli insituelectroporationofmammaliancellsthroughsio2thinfilmcapacitivemicroelectrodes
_version_	1718384015777464320

In situ electroporation of mammalian cells through SiO2 thin film capacitive microelectrodes

Ejemplares similares