Development of a Quantitative Method for Analysis of Compounds Found in <i>Mondia whitei</i> Using HPLC-DAD

<i>Mondia whitei</i> is one of the popular medicinal plants in Africa, which has been used extensively across the continent to cure various ailments. Products prepared from this plant are distributed commercially. In this study, an HPLC-DAD method was developed and validated for seven co...

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Autores principales:	Ramakwala Christinah Chokwe, Simiso Dube, Mathew Muzi Nindi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Mondia whitei</i> HPLC-DAD method development quality control one-factor-at-a-time Box–Behnken design Chemical technology TP1-1185 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/c3b9b1d9eef34faf9cc9181fb4d8ec7a
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id	oai:doaj.org-article:c3b9b1d9eef34faf9cc9181fb4d8ec7a
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spelling	oai:doaj.org-article:c3b9b1d9eef34faf9cc9181fb4d8ec7a2021-11-25T18:49:53ZDevelopment of a Quantitative Method for Analysis of Compounds Found in <i>Mondia whitei</i> Using HPLC-DAD10.3390/pr91118642227-9717https://doaj.org/article/c3b9b1d9eef34faf9cc9181fb4d8ec7a2021-10-01T00:00:00Zhttps://www.mdpi.com/2227-9717/9/11/1864https://doaj.org/toc/2227-9717<i>Mondia whitei</i> is one of the popular medicinal plants in Africa, which has been used extensively across the continent to cure various ailments. Products prepared from this plant are distributed commercially. In this study, an HPLC-DAD method was developed and validated for seven compounds to enable quality control of these products. The method was validated using the external standard-calibration and the standard-addition methods which were found not significantly different. The method was found to be linear in the ranges 1–14 mg/L and 3–14 mg/kg with correlation coefficients from 0.9942–0.9995 and 0.9907–0.9993 in the solvent and the different matrices, respectively. The limit of detection (LOD) values of the method were found to range between 0.1–1.2 mg/L in the solvent and syrup, and between 0.4–1.2 mg/kg in the teabag and root powder. The limit of quantification (LOQ) values were found to range between 0.5–3.9 mg/L in the solvent and syrup, and between 1.4–3.8 mg/kg in the teabag and root powder. The method was also found to be precise with percentage relative standard deviations below 5% and 10% in the solvent and matrices, respectively. The method was applied to <i>Mondia whitei</i> products for qualitative and quantitative analysis.Ramakwala Christinah ChokweSimiso DubeMathew Muzi NindiMDPI AGarticle<i>Mondia whitei</i>HPLC-DAD method developmentquality controlone-factor-at-a-timeBox–Behnken designChemical technologyTP1-1185ChemistryQD1-999ENProcesses, Vol 9, Iss 1864, p 1864 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Mondia whitei</i> HPLC-DAD method development quality control one-factor-at-a-time Box–Behnken design Chemical technology TP1-1185 Chemistry QD1-999
spellingShingle	<i>Mondia whitei</i> HPLC-DAD method development quality control one-factor-at-a-time Box–Behnken design Chemical technology TP1-1185 Chemistry QD1-999 Ramakwala Christinah Chokwe Simiso Dube Mathew Muzi Nindi Development of a Quantitative Method for Analysis of Compounds Found in <i>Mondia whitei</i> Using HPLC-DAD
description	<i>Mondia whitei</i> is one of the popular medicinal plants in Africa, which has been used extensively across the continent to cure various ailments. Products prepared from this plant are distributed commercially. In this study, an HPLC-DAD method was developed and validated for seven compounds to enable quality control of these products. The method was validated using the external standard-calibration and the standard-addition methods which were found not significantly different. The method was found to be linear in the ranges 1–14 mg/L and 3–14 mg/kg with correlation coefficients from 0.9942–0.9995 and 0.9907–0.9993 in the solvent and the different matrices, respectively. The limit of detection (LOD) values of the method were found to range between 0.1–1.2 mg/L in the solvent and syrup, and between 0.4–1.2 mg/kg in the teabag and root powder. The limit of quantification (LOQ) values were found to range between 0.5–3.9 mg/L in the solvent and syrup, and between 1.4–3.8 mg/kg in the teabag and root powder. The method was also found to be precise with percentage relative standard deviations below 5% and 10% in the solvent and matrices, respectively. The method was applied to <i>Mondia whitei</i> products for qualitative and quantitative analysis.
format	article
author	Ramakwala Christinah Chokwe Simiso Dube Mathew Muzi Nindi
author_facet	Ramakwala Christinah Chokwe Simiso Dube Mathew Muzi Nindi
author_sort	Ramakwala Christinah Chokwe
title	Development of a Quantitative Method for Analysis of Compounds Found in <i>Mondia whitei</i> Using HPLC-DAD
title_short	Development of a Quantitative Method for Analysis of Compounds Found in <i>Mondia whitei</i> Using HPLC-DAD
title_full	Development of a Quantitative Method for Analysis of Compounds Found in <i>Mondia whitei</i> Using HPLC-DAD
title_fullStr	Development of a Quantitative Method for Analysis of Compounds Found in <i>Mondia whitei</i> Using HPLC-DAD
title_full_unstemmed	Development of a Quantitative Method for Analysis of Compounds Found in <i>Mondia whitei</i> Using HPLC-DAD
title_sort	development of a quantitative method for analysis of compounds found in <i>mondia whitei</i> using hplc-dad
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c3b9b1d9eef34faf9cc9181fb4d8ec7a
work_keys_str_mv	AT ramakwalachristinahchokwe developmentofaquantitativemethodforanalysisofcompoundsfoundinimondiawhiteiiusinghplcdad AT simisodube developmentofaquantitativemethodforanalysisofcompoundsfoundinimondiawhiteiiusinghplcdad AT mathewmuzinindi developmentofaquantitativemethodforanalysisofcompoundsfoundinimondiawhiteiiusinghplcdad
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Development of a Quantitative Method for Analysis of Compounds Found in <i>Mondia whitei</i> Using HPLC-DAD

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