Oral treatment with herbal formula B401 alleviates penile toxicity in aging mice with manganism

Chih-Hsiang Hsu,1 Ching-Lung Lin,1 Sheue-Er Wang,1 Shuenn-Jyi Sheu,2 Chiang-Ting Chien,1 Chung-Hsin Wu1 1Department of Life Science, National Taiwan Normal University, 2Brion Research Institute of Taiwan, Taipei, Taiwan Abstract: The present study aims to elucidate the roles of nitric oxide syntha...

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Autores principales: Hsu CH, Lin CL, Wang SE, Sheu SJ, Chien CT, Wu CH
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Publicado: Dove Medical Press 2015
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spelling oai:doaj.org-article:c3ba6cb5e2b84624a7d7a74aaf8e96132021-12-02T00:38:38ZOral treatment with herbal formula B401 alleviates penile toxicity in aging mice with manganism1178-1998https://doaj.org/article/c3ba6cb5e2b84624a7d7a74aaf8e96132015-05-01T00:00:00Zhttps://www.dovepress.com/oral-treatment-with-herbal-formula-b401-alleviates-penile-toxicity-in--peer-reviewed-article-CIAhttps://doaj.org/toc/1178-1998Chih-Hsiang Hsu,1 Ching-Lung Lin,1 Sheue-Er Wang,1 Shuenn-Jyi Sheu,2 Chiang-Ting Chien,1 Chung-Hsin Wu1 1Department of Life Science, National Taiwan Normal University, 2Brion Research Institute of Taiwan, Taipei, Taiwan Abstract: The present study aims to elucidate the roles of nitric oxide synthase activity, oxidative stress, inflammation, and apoptosis in penile toxicity of aging mice associated with excess manganese (Mn) treatment and to investigate the effect of oral treatment with the herbal formula B401 in this respect. ICR strain mice were divided into two groups: the vehicle (sham group) and the B401 (50 mg/kg) group. The mice were orally treated for 5 days; then a high single dose of MnCl2 (100 mg/kg) was given by intraperitoneal injection to the mice. One day after MnCl2 treatment, corpora cavernosal tissues of both Mn-treated mice and their controls were simultaneously sampled to examine their immunohistochemical staining and Western blot analysis. Nitric oxide (NO) production, levels of neuronal nitric oxide synthase (nNOS) and endothelial nitric oxide synthase (eNOS), expression levels of factors governing angiogenesis (vascular endothelial growth factor), oxidative stress (catalase, superoxide dismutase 2,4-hydroxynonenal), inflammation (tumor necrosis factor alpha), apoptosis (B-cell lymphoma 2 [Bcl-2], Bcl-2-associated X protein [Bax], cleaved poly(adenosine diphosphate-ribose) polymerase [c-PARP], cytochrome C, caspase-12, and caspase-3) were evaluated in penile corpus cavernosum of the mice. We found that penile toxicity in the mice was enhanced under excess Mn treatment through reduction of NOS activity and increase in oxidative stress, inflammation, and apoptosis in the penile cavernous tissue. Furthermore, the penile toxicity in mice with manganism was alleviated by oral B401 treatment through enhancement of both nitric oxide synthesis and angiogenesis, with simultaneous reduction of oxidative stress, inflammation, and apoptosis in penile corpus cavernosum. We suggest that the herbal formula B401 may serve as a potential dietotherapeutic supplement for penile toxicity or dysfunction in aging males. Keywords: manganism, herbal formula, oxidative stress, inflammation, apoptosisHsu CHLin CLWang SESheu SJChien CTWu CHDove Medical Pressarticlepenile manganismherbal formulanitric oxide signalingoxidative stressinflammationapoptosisaging miceGeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 10, Pp 907-918 (2015)
institution DOAJ
collection DOAJ
language EN
topic penile manganism
herbal formula
nitric oxide signaling
oxidative stress
inflammation
apoptosis
aging mice
Geriatrics
RC952-954.6
spellingShingle penile manganism
herbal formula
nitric oxide signaling
oxidative stress
inflammation
apoptosis
aging mice
Geriatrics
RC952-954.6
Hsu CH
Lin CL
Wang SE
Sheu SJ
Chien CT
Wu CH
Oral treatment with herbal formula B401 alleviates penile toxicity in aging mice with manganism
description Chih-Hsiang Hsu,1 Ching-Lung Lin,1 Sheue-Er Wang,1 Shuenn-Jyi Sheu,2 Chiang-Ting Chien,1 Chung-Hsin Wu1 1Department of Life Science, National Taiwan Normal University, 2Brion Research Institute of Taiwan, Taipei, Taiwan Abstract: The present study aims to elucidate the roles of nitric oxide synthase activity, oxidative stress, inflammation, and apoptosis in penile toxicity of aging mice associated with excess manganese (Mn) treatment and to investigate the effect of oral treatment with the herbal formula B401 in this respect. ICR strain mice were divided into two groups: the vehicle (sham group) and the B401 (50 mg/kg) group. The mice were orally treated for 5 days; then a high single dose of MnCl2 (100 mg/kg) was given by intraperitoneal injection to the mice. One day after MnCl2 treatment, corpora cavernosal tissues of both Mn-treated mice and their controls were simultaneously sampled to examine their immunohistochemical staining and Western blot analysis. Nitric oxide (NO) production, levels of neuronal nitric oxide synthase (nNOS) and endothelial nitric oxide synthase (eNOS), expression levels of factors governing angiogenesis (vascular endothelial growth factor), oxidative stress (catalase, superoxide dismutase 2,4-hydroxynonenal), inflammation (tumor necrosis factor alpha), apoptosis (B-cell lymphoma 2 [Bcl-2], Bcl-2-associated X protein [Bax], cleaved poly(adenosine diphosphate-ribose) polymerase [c-PARP], cytochrome C, caspase-12, and caspase-3) were evaluated in penile corpus cavernosum of the mice. We found that penile toxicity in the mice was enhanced under excess Mn treatment through reduction of NOS activity and increase in oxidative stress, inflammation, and apoptosis in the penile cavernous tissue. Furthermore, the penile toxicity in mice with manganism was alleviated by oral B401 treatment through enhancement of both nitric oxide synthesis and angiogenesis, with simultaneous reduction of oxidative stress, inflammation, and apoptosis in penile corpus cavernosum. We suggest that the herbal formula B401 may serve as a potential dietotherapeutic supplement for penile toxicity or dysfunction in aging males. Keywords: manganism, herbal formula, oxidative stress, inflammation, apoptosis
format article
author Hsu CH
Lin CL
Wang SE
Sheu SJ
Chien CT
Wu CH
author_facet Hsu CH
Lin CL
Wang SE
Sheu SJ
Chien CT
Wu CH
author_sort Hsu CH
title Oral treatment with herbal formula B401 alleviates penile toxicity in aging mice with manganism
title_short Oral treatment with herbal formula B401 alleviates penile toxicity in aging mice with manganism
title_full Oral treatment with herbal formula B401 alleviates penile toxicity in aging mice with manganism
title_fullStr Oral treatment with herbal formula B401 alleviates penile toxicity in aging mice with manganism
title_full_unstemmed Oral treatment with herbal formula B401 alleviates penile toxicity in aging mice with manganism
title_sort oral treatment with herbal formula b401 alleviates penile toxicity in aging mice with manganism
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/c3ba6cb5e2b84624a7d7a74aaf8e9613
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