Effluent and serum protein N-glycosylation is associated with inflammation and peritoneal membrane transport characteristics in peritoneal dialysis patients

Abstract Mass spectrometric glycomics was used as an innovative approach to identify biomarkers in serum and dialysate samples from peritoneal dialysis (PD) patients. PD is a life-saving treatment worldwide applied in more than 100,000 patients suffering from chronic kidney disease. PD treatment use...

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Autores principales:	Evelina Ferrantelli, Karima Farhat, Agnes L. Hipgrave Ederveen, Karli R. Reiding, Robert H. J. Beelen, Frans J. van Ittersum, Manfred Wuhrer, Viktoria Dotz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c3bd45e874fd4b6eb24f1f6192150d5e
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spelling	oai:doaj.org-article:c3bd45e874fd4b6eb24f1f6192150d5e2021-12-02T15:07:49ZEffluent and serum protein N-glycosylation is associated with inflammation and peritoneal membrane transport characteristics in peritoneal dialysis patients10.1038/s41598-018-19147-x2045-2322https://doaj.org/article/c3bd45e874fd4b6eb24f1f6192150d5e2018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19147-xhttps://doaj.org/toc/2045-2322Abstract Mass spectrometric glycomics was used as an innovative approach to identify biomarkers in serum and dialysate samples from peritoneal dialysis (PD) patients. PD is a life-saving treatment worldwide applied in more than 100,000 patients suffering from chronic kidney disease. PD treatment uses the peritoneum as a natural membrane to exchange waste products from blood to a glucose-based solution. Daily exposure of the peritoneal membrane to these solutions may cause complications such as peritonitis, fibrosis and inflammation which, in the long term, lead to the failure of the treatment. It has been shown in the last years that protein N-glycosylation is related to inflammatory and fibrotic processes. Here, by using a recently developed MALDI-TOF-MS method with linkage-specific sialic acid derivatisation, we showed that alpha2,6-sialylation, especially in triantennary N-glycans from peritoneal effluents, is associated with critical clinical outcomes in a prospective cohort of 94 PD patients. Moreover, we found an association between the levels of presumably immunoglobulin-G-related glycans as well as galactosylation of diantennary glycans with PD-related complications such as peritonitis and loss of peritoneal mesothelial cell mass. The observed glycomic changes point to changes in protein abundance and protein-specific glycosylation, representing candidate functional biomarkers of PD and associated complications.Evelina FerrantelliKarima FarhatAgnes L. Hipgrave EderveenKarli R. ReidingRobert H. J. BeelenFrans J. van IttersumManfred WuhrerViktoria DotzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Evelina Ferrantelli Karima Farhat Agnes L. Hipgrave Ederveen Karli R. Reiding Robert H. J. Beelen Frans J. van Ittersum Manfred Wuhrer Viktoria Dotz Effluent and serum protein N-glycosylation is associated with inflammation and peritoneal membrane transport characteristics in peritoneal dialysis patients
description	Abstract Mass spectrometric glycomics was used as an innovative approach to identify biomarkers in serum and dialysate samples from peritoneal dialysis (PD) patients. PD is a life-saving treatment worldwide applied in more than 100,000 patients suffering from chronic kidney disease. PD treatment uses the peritoneum as a natural membrane to exchange waste products from blood to a glucose-based solution. Daily exposure of the peritoneal membrane to these solutions may cause complications such as peritonitis, fibrosis and inflammation which, in the long term, lead to the failure of the treatment. It has been shown in the last years that protein N-glycosylation is related to inflammatory and fibrotic processes. Here, by using a recently developed MALDI-TOF-MS method with linkage-specific sialic acid derivatisation, we showed that alpha2,6-sialylation, especially in triantennary N-glycans from peritoneal effluents, is associated with critical clinical outcomes in a prospective cohort of 94 PD patients. Moreover, we found an association between the levels of presumably immunoglobulin-G-related glycans as well as galactosylation of diantennary glycans with PD-related complications such as peritonitis and loss of peritoneal mesothelial cell mass. The observed glycomic changes point to changes in protein abundance and protein-specific glycosylation, representing candidate functional biomarkers of PD and associated complications.
format	article
author	Evelina Ferrantelli Karima Farhat Agnes L. Hipgrave Ederveen Karli R. Reiding Robert H. J. Beelen Frans J. van Ittersum Manfred Wuhrer Viktoria Dotz
author_facet	Evelina Ferrantelli Karima Farhat Agnes L. Hipgrave Ederveen Karli R. Reiding Robert H. J. Beelen Frans J. van Ittersum Manfred Wuhrer Viktoria Dotz
author_sort	Evelina Ferrantelli
title	Effluent and serum protein N-glycosylation is associated with inflammation and peritoneal membrane transport characteristics in peritoneal dialysis patients
title_short	Effluent and serum protein N-glycosylation is associated with inflammation and peritoneal membrane transport characteristics in peritoneal dialysis patients
title_full	Effluent and serum protein N-glycosylation is associated with inflammation and peritoneal membrane transport characteristics in peritoneal dialysis patients
title_fullStr	Effluent and serum protein N-glycosylation is associated with inflammation and peritoneal membrane transport characteristics in peritoneal dialysis patients
title_full_unstemmed	Effluent and serum protein N-glycosylation is associated with inflammation and peritoneal membrane transport characteristics in peritoneal dialysis patients
title_sort	effluent and serum protein n-glycosylation is associated with inflammation and peritoneal membrane transport characteristics in peritoneal dialysis patients
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/c3bd45e874fd4b6eb24f1f6192150d5e
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Effluent and serum protein N-glycosylation is associated with inflammation and peritoneal membrane transport characteristics in peritoneal dialysis patients

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