Rifampicin conjugated silver nanoparticles: a new arena for development of antibiofilm potential against methicillin resistant Staphylococcus aureus and Klebsiella pneumoniae

Umar Farooq,1,* Touqeer Ahmad,1,* Ajmal Khan,2 Rizwana Sarwar,1 Jazib Shafiq,3 Yasir Raza,4 Ayaz Ahmed,3 Safi Ullah,5 Najeeb Ur Rehman,2 Ahmed Al-Harrasi21Department of Chemistry, COMSATS University Islamabad Abbottabad Campus, Abbottabad, Pakistan; 2Natural and Medical Sciences Research Center, Uni...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Farooq U, Ahmad T, Khan A, Sarwar R, Shafiq J, Raza Y, Ahmed A, Ullah S, Ur Rehman N, Al-Harrasi A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Rifampicin Silver nanoparticles TEM AFM Antibiofilm Staphylococcus aureus and Klebsiella pneumoniae Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c3c204176e954bc5829e8c53f989b962
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c3c204176e954bc5829e8c53f989b962
record_format	dspace
spelling	oai:doaj.org-article:c3c204176e954bc5829e8c53f989b9622021-12-02T10:04:02ZRifampicin conjugated silver nanoparticles: a new arena for development of antibiofilm potential against methicillin resistant Staphylococcus aureus and Klebsiella pneumoniae1178-2013https://doaj.org/article/c3c204176e954bc5829e8c53f989b9622019-05-01T00:00:00Zhttps://www.dovepress.com/rifampicin-conjugated-silver-nanoparticles-a-new-arena-for-development-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Umar Farooq,1,* Touqeer Ahmad,1,* Ajmal Khan,2 Rizwana Sarwar,1 Jazib Shafiq,3 Yasir Raza,4 Ayaz Ahmed,3 Safi Ullah,5 Najeeb Ur Rehman,2 Ahmed Al-Harrasi21Department of Chemistry, COMSATS University Islamabad Abbottabad Campus, Abbottabad, Pakistan; 2Natural and Medical Sciences Research Center, University of Nizwa, Nizwa, Sultanate of Oman; 3Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan; 4Department of Microbiology, University of Karachi, Karachi, Pakistan; 5Department of Pharmacy, University of Peshawar, Peshawar, Pakistan*These authors contributed equally to this workBackground: Infections caused by drug resistant bacteria are a major health concern worldwide and have prompted scientists to carry out efforts to overcome this challenge. Researchers and pharmaceutical companies are trying to develop new kinds of antimicrobial agents by using different physical and chemical methods to overcome these problems.Materials and methods: In the present study, rifampicin conjugated silver (Rif-Ag) nanoparticles have successfully been synthesized using a chemical method. Characterization of the nanoparticles was performed using a UV-Vis spectrophotometer, FTIR, SEM, TEM, and AFM.Results: The AFM, SEM, and TEM results showed that the average particle size of Rif-Ag nanoparticles was about 15–18±4 nm. The FTIR spectra revealed the conjugation of –NH2 and –OH functional moiety with silver nanoparticles surface. Considering the penetrating power of rifampicin, the free drug is compared with synthesized nanoparticle for antimicrobial, biofilm inhibition, and eradication potential. Synthesized nanoparticles were found to be significantly active as compared to drug alone.Conclusion: This study has shown greater biofilm inhibitory and eradicating potential against methicillin resistant Staphylococcus aureus and Klebsiella pneumoniae, as evident by crystal violet, MTT staining, and microscopic analysis. So, it will be further modified, and studies for the mechanism of action are needed.Keywords: rifampicin, silver nanoparticles, TEM, AFM, antibiofilm, Staphylococcus aureus, Klebsiella pneumoniaeFarooq UAhmad TKhan ASarwar RShafiq JRaza YAhmed AUllah SUr Rehman NAl-Harrasi ADove Medical PressarticleRifampicinSilver nanoparticlesTEMAFMAntibiofilmStaphylococcus aureus and Klebsiella pneumoniaeMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 3983-3993 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Rifampicin Silver nanoparticles TEM AFM Antibiofilm Staphylococcus aureus and Klebsiella pneumoniae Medicine (General) R5-920
spellingShingle	Rifampicin Silver nanoparticles TEM AFM Antibiofilm Staphylococcus aureus and Klebsiella pneumoniae Medicine (General) R5-920 Farooq U Ahmad T Khan A Sarwar R Shafiq J Raza Y Ahmed A Ullah S Ur Rehman N Al-Harrasi A Rifampicin conjugated silver nanoparticles: a new arena for development of antibiofilm potential against methicillin resistant Staphylococcus aureus and Klebsiella pneumoniae
description	Umar Farooq,1,* Touqeer Ahmad,1,* Ajmal Khan,2 Rizwana Sarwar,1 Jazib Shafiq,3 Yasir Raza,4 Ayaz Ahmed,3 Safi Ullah,5 Najeeb Ur Rehman,2 Ahmed Al-Harrasi21Department of Chemistry, COMSATS University Islamabad Abbottabad Campus, Abbottabad, Pakistan; 2Natural and Medical Sciences Research Center, University of Nizwa, Nizwa, Sultanate of Oman; 3Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan; 4Department of Microbiology, University of Karachi, Karachi, Pakistan; 5Department of Pharmacy, University of Peshawar, Peshawar, Pakistan*These authors contributed equally to this workBackground: Infections caused by drug resistant bacteria are a major health concern worldwide and have prompted scientists to carry out efforts to overcome this challenge. Researchers and pharmaceutical companies are trying to develop new kinds of antimicrobial agents by using different physical and chemical methods to overcome these problems.Materials and methods: In the present study, rifampicin conjugated silver (Rif-Ag) nanoparticles have successfully been synthesized using a chemical method. Characterization of the nanoparticles was performed using a UV-Vis spectrophotometer, FTIR, SEM, TEM, and AFM.Results: The AFM, SEM, and TEM results showed that the average particle size of Rif-Ag nanoparticles was about 15–18±4 nm. The FTIR spectra revealed the conjugation of –NH2 and –OH functional moiety with silver nanoparticles surface. Considering the penetrating power of rifampicin, the free drug is compared with synthesized nanoparticle for antimicrobial, biofilm inhibition, and eradication potential. Synthesized nanoparticles were found to be significantly active as compared to drug alone.Conclusion: This study has shown greater biofilm inhibitory and eradicating potential against methicillin resistant Staphylococcus aureus and Klebsiella pneumoniae, as evident by crystal violet, MTT staining, and microscopic analysis. So, it will be further modified, and studies for the mechanism of action are needed.Keywords: rifampicin, silver nanoparticles, TEM, AFM, antibiofilm, Staphylococcus aureus, Klebsiella pneumoniae
format	article
author	Farooq U Ahmad T Khan A Sarwar R Shafiq J Raza Y Ahmed A Ullah S Ur Rehman N Al-Harrasi A
author_facet	Farooq U Ahmad T Khan A Sarwar R Shafiq J Raza Y Ahmed A Ullah S Ur Rehman N Al-Harrasi A
author_sort	Farooq U
title	Rifampicin conjugated silver nanoparticles: a new arena for development of antibiofilm potential against methicillin resistant Staphylococcus aureus and Klebsiella pneumoniae
title_short	Rifampicin conjugated silver nanoparticles: a new arena for development of antibiofilm potential against methicillin resistant Staphylococcus aureus and Klebsiella pneumoniae
title_full	Rifampicin conjugated silver nanoparticles: a new arena for development of antibiofilm potential against methicillin resistant Staphylococcus aureus and Klebsiella pneumoniae
title_fullStr	Rifampicin conjugated silver nanoparticles: a new arena for development of antibiofilm potential against methicillin resistant Staphylococcus aureus and Klebsiella pneumoniae
title_full_unstemmed	Rifampicin conjugated silver nanoparticles: a new arena for development of antibiofilm potential against methicillin resistant Staphylococcus aureus and Klebsiella pneumoniae
title_sort	rifampicin conjugated silver nanoparticles: a new arena for development of antibiofilm potential against methicillin resistant staphylococcus aureus and klebsiella pneumoniae
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/c3c204176e954bc5829e8c53f989b962
work_keys_str_mv	AT farooqu rifampicinconjugatedsilvernanoparticlesanewarenafordevelopmentofantibiofilmpotentialagainstmethicillinresistantstaphylococcusaureusandklebsiellapneumoniae AT ahmadt rifampicinconjugatedsilvernanoparticlesanewarenafordevelopmentofantibiofilmpotentialagainstmethicillinresistantstaphylococcusaureusandklebsiellapneumoniae AT khana rifampicinconjugatedsilvernanoparticlesanewarenafordevelopmentofantibiofilmpotentialagainstmethicillinresistantstaphylococcusaureusandklebsiellapneumoniae AT sarwarr rifampicinconjugatedsilvernanoparticlesanewarenafordevelopmentofantibiofilmpotentialagainstmethicillinresistantstaphylococcusaureusandklebsiellapneumoniae AT shafiqj rifampicinconjugatedsilvernanoparticlesanewarenafordevelopmentofantibiofilmpotentialagainstmethicillinresistantstaphylococcusaureusandklebsiellapneumoniae AT razay rifampicinconjugatedsilvernanoparticlesanewarenafordevelopmentofantibiofilmpotentialagainstmethicillinresistantstaphylococcusaureusandklebsiellapneumoniae AT ahmeda rifampicinconjugatedsilvernanoparticlesanewarenafordevelopmentofantibiofilmpotentialagainstmethicillinresistantstaphylococcusaureusandklebsiellapneumoniae AT ullahs rifampicinconjugatedsilvernanoparticlesanewarenafordevelopmentofantibiofilmpotentialagainstmethicillinresistantstaphylococcusaureusandklebsiellapneumoniae AT urrehmann rifampicinconjugatedsilvernanoparticlesanewarenafordevelopmentofantibiofilmpotentialagainstmethicillinresistantstaphylococcusaureusandklebsiellapneumoniae AT alharrasia rifampicinconjugatedsilvernanoparticlesanewarenafordevelopmentofantibiofilmpotentialagainstmethicillinresistantstaphylococcusaureusandklebsiellapneumoniae
_version_	1718397709945143296

Rifampicin conjugated silver nanoparticles: a new arena for development of antibiofilm potential against methicillin resistant Staphylococcus aureus and Klebsiella pneumoniae

Ejemplares similares