Elementary calcium release events in the skeletal muscle cells of the honey bee Apis mellifera

Abstract Calcium sparks are involved in major physiological and pathological processes in vertebrate muscles but have never been characterized in invertebrates. Here, dynamic confocal imaging on intact skeletal muscle cells isolated enzymatically from the adult honey bee legs allowed the first spati...

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Autores principales:	Claude Collet, Mercédès Charreton, Laszlo Szabo, Marianna Takacs, Laszlo Csernoch, Peter Szentesi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c3cd5eabd6544121b43c0d5cdd28b3f2
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spelling	oai:doaj.org-article:c3cd5eabd6544121b43c0d5cdd28b3f22021-12-02T18:51:52ZElementary calcium release events in the skeletal muscle cells of the honey bee Apis mellifera10.1038/s41598-021-96028-w2045-2322https://doaj.org/article/c3cd5eabd6544121b43c0d5cdd28b3f22021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96028-whttps://doaj.org/toc/2045-2322Abstract Calcium sparks are involved in major physiological and pathological processes in vertebrate muscles but have never been characterized in invertebrates. Here, dynamic confocal imaging on intact skeletal muscle cells isolated enzymatically from the adult honey bee legs allowed the first spatio-temporal characterization of subcellular calcium release events (CREs) in an insect species. The frequency of CREs, measured in x–y time lapse series, was higher than frequencies usually described in vertebrates. Honey bee CREs had a larger spatial spread at half maximum than their vertebrate counterparts and a slightly ellipsoidal shape, two characteristics that may be related to ultrastructural features specific to invertebrate cells. In line-scan experiments, the histogram of CREs’ duration followed a bimodal distribution, supporting the existence of both sparks and embers. Unlike in vertebrates, embers and sparks had similar amplitudes, a difference that could be related to genomic differences and/or excitation–contraction coupling specificities in honey bee skeletal muscle fibres. The first characterization of CREs from an arthropod which shows strong genomic, ultrastructural and physiological differences with vertebrates may help in improving the research field of sparkology and more generally the knowledge in invertebrates cell Ca2+ homeostasis, eventually leading to a better understanding of their roles and regulations in muscles but also the myotoxicity of new insecticides targeting ryanodine receptors.Claude ColletMercédès CharretonLaszlo SzaboMarianna TakacsLaszlo CsernochPeter SzentesiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Claude Collet Mercédès Charreton Laszlo Szabo Marianna Takacs Laszlo Csernoch Peter Szentesi Elementary calcium release events in the skeletal muscle cells of the honey bee Apis mellifera
description	Abstract Calcium sparks are involved in major physiological and pathological processes in vertebrate muscles but have never been characterized in invertebrates. Here, dynamic confocal imaging on intact skeletal muscle cells isolated enzymatically from the adult honey bee legs allowed the first spatio-temporal characterization of subcellular calcium release events (CREs) in an insect species. The frequency of CREs, measured in x–y time lapse series, was higher than frequencies usually described in vertebrates. Honey bee CREs had a larger spatial spread at half maximum than their vertebrate counterparts and a slightly ellipsoidal shape, two characteristics that may be related to ultrastructural features specific to invertebrate cells. In line-scan experiments, the histogram of CREs’ duration followed a bimodal distribution, supporting the existence of both sparks and embers. Unlike in vertebrates, embers and sparks had similar amplitudes, a difference that could be related to genomic differences and/or excitation–contraction coupling specificities in honey bee skeletal muscle fibres. The first characterization of CREs from an arthropod which shows strong genomic, ultrastructural and physiological differences with vertebrates may help in improving the research field of sparkology and more generally the knowledge in invertebrates cell Ca2+ homeostasis, eventually leading to a better understanding of their roles and regulations in muscles but also the myotoxicity of new insecticides targeting ryanodine receptors.
format	article
author	Claude Collet Mercédès Charreton Laszlo Szabo Marianna Takacs Laszlo Csernoch Peter Szentesi
author_facet	Claude Collet Mercédès Charreton Laszlo Szabo Marianna Takacs Laszlo Csernoch Peter Szentesi
author_sort	Claude Collet
title	Elementary calcium release events in the skeletal muscle cells of the honey bee Apis mellifera
title_short	Elementary calcium release events in the skeletal muscle cells of the honey bee Apis mellifera
title_full	Elementary calcium release events in the skeletal muscle cells of the honey bee Apis mellifera
title_fullStr	Elementary calcium release events in the skeletal muscle cells of the honey bee Apis mellifera
title_full_unstemmed	Elementary calcium release events in the skeletal muscle cells of the honey bee Apis mellifera
title_sort	elementary calcium release events in the skeletal muscle cells of the honey bee apis mellifera
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c3cd5eabd6544121b43c0d5cdd28b3f2
work_keys_str_mv	AT claudecollet elementarycalciumreleaseeventsintheskeletalmusclecellsofthehoneybeeapismellifera AT mercedescharreton elementarycalciumreleaseeventsintheskeletalmusclecellsofthehoneybeeapismellifera AT laszloszabo elementarycalciumreleaseeventsintheskeletalmusclecellsofthehoneybeeapismellifera AT mariannatakacs elementarycalciumreleaseeventsintheskeletalmusclecellsofthehoneybeeapismellifera AT laszlocsernoch elementarycalciumreleaseeventsintheskeletalmusclecellsofthehoneybeeapismellifera AT peterszentesi elementarycalciumreleaseeventsintheskeletalmusclecellsofthehoneybeeapismellifera
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Elementary calcium release events in the skeletal muscle cells of the honey bee Apis mellifera

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