In Silico and In Vitro Evaluation of the Antimicrobial Potential of Bacillus cereus Isolated from Apis dorsata Gut against Neisseria gonorrhoeae

Antimicrobial resistance is a major public health and development concern on a global scale. The increasing resistance of the pathogenic bacteria Neisseria gonorrhoeae to antibiotics necessitates efforts to identify potential alternative antibiotics from nature, including insects,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nurdjannah Jane Niode, Aryani Adji, Jimmy Rimbing, Max Tulung, Mohammed Alorabi, Ahmed M. El-Shehawi, Rinaldi Idroes, Ismail Celik, Fatimawali, Ahmad Akroman Adam, Kuldeep Dhama, Gomaa Mostafa-Hedeab, Amany Abdel-Rahman Mohamed, Trina Ekawati Tallei, Talha Bin Emran
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	lipopeptide <i>Apis dorsata</i> <i>Bacillus cereus</i> <i>Neisseria gonorrhoeae</i> insect gut antimicrobial activity Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/c3cf706a559442dd9eb7556165977105
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c3cf706a559442dd9eb7556165977105
record_format	dspace
spelling	oai:doaj.org-article:c3cf706a559442dd9eb75561659771052021-11-25T16:24:47ZIn Silico and In Vitro Evaluation of the Antimicrobial Potential of <i>Bacillus cereus</i> Isolated from <i>Apis dorsata</i> Gut against <i>Neisseria gonorrhoeae</i>10.3390/antibiotics101114012079-6382https://doaj.org/article/c3cf706a559442dd9eb75561659771052021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1401https://doaj.org/toc/2079-6382Antimicrobial resistance is a major public health and development concern on a global scale. The increasing resistance of the pathogenic bacteria <i>Neisseria gonorrhoeae</i> to antibiotics necessitates efforts to identify potential alternative antibiotics from nature, including insects, which are already recognized as a source of natural antibiotics by the scientific community. This study aimed to determine the potential of components of gut-associated bacteria isolated from <i>Apis dorsata</i>, an Asian giant honeybee, as an antibacterial against <i>N. gonorrhoeae</i> by in vitro and in silico methods as an initial process in the stage of new drug discovery. The identified gut-associated bacteria of <i>A. dorsata</i> included <i>Acinetobacter indicus</i> and <i>Bacillus cereus</i> with 100% identity to referenced bacteria from GenBank. Cell-free culture supernatants (CFCS) of <i>B. cereus</i> had a very strong antibacterial activity against <i>N. gonorrhoeae</i> in an in vitro antibacterial testing. Meanwhile, molecular docking revealed that antimicrobial lipopeptides from <i>B. cereus</i> (surfactin, fengycin, and iturin A) had a comparable value of binding-free energy (BFE) with the target protein receptor for <i>N. gonorrhoeae</i>, namely penicillin-binding protein (PBP) 1 and PBP2 when compared with the ceftriaxone, cefixime, and doxycycline. The molecular dynamics simulation (MDS) study revealed that the surfactin remains stable at the active site of PBP2 despite the alteration of the H-bond and hydrophobic interactions. According to this finding, surfactin has the greatest antibacterial potential against PBP2 of <i>N. gonorrhoeae</i>.Nurdjannah Jane NiodeAryani AdjiJimmy RimbingMax TulungMohammed AlorabiAhmed M. El-ShehawiRinaldi IdroesIsmail CelikFatimawaliAhmad Akroman AdamKuldeep DhamaGomaa Mostafa-HedeabAmany Abdel-Rahman MohamedTrina Ekawati TalleiTalha Bin EmranMDPI AGarticlelipopeptide<i>Apis dorsata</i><i>Bacillus cereus</i><i>Neisseria gonorrhoeae</i>insect gutantimicrobial activityTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1401, p 1401 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lipopeptide <i>Apis dorsata</i> <i>Bacillus cereus</i> <i>Neisseria gonorrhoeae</i> insect gut antimicrobial activity Therapeutics. Pharmacology RM1-950
spellingShingle	lipopeptide <i>Apis dorsata</i> <i>Bacillus cereus</i> <i>Neisseria gonorrhoeae</i> insect gut antimicrobial activity Therapeutics. Pharmacology RM1-950 Nurdjannah Jane Niode Aryani Adji Jimmy Rimbing Max Tulung Mohammed Alorabi Ahmed M. El-Shehawi Rinaldi Idroes Ismail Celik Fatimawali Ahmad Akroman Adam Kuldeep Dhama Gomaa Mostafa-Hedeab Amany Abdel-Rahman Mohamed Trina Ekawati Tallei Talha Bin Emran In Silico and In Vitro Evaluation of the Antimicrobial Potential of <i>Bacillus cereus</i> Isolated from <i>Apis dorsata</i> Gut against <i>Neisseria gonorrhoeae</i>
description	Antimicrobial resistance is a major public health and development concern on a global scale. The increasing resistance of the pathogenic bacteria <i>Neisseria gonorrhoeae</i> to antibiotics necessitates efforts to identify potential alternative antibiotics from nature, including insects, which are already recognized as a source of natural antibiotics by the scientific community. This study aimed to determine the potential of components of gut-associated bacteria isolated from <i>Apis dorsata</i>, an Asian giant honeybee, as an antibacterial against <i>N. gonorrhoeae</i> by in vitro and in silico methods as an initial process in the stage of new drug discovery. The identified gut-associated bacteria of <i>A. dorsata</i> included <i>Acinetobacter indicus</i> and <i>Bacillus cereus</i> with 100% identity to referenced bacteria from GenBank. Cell-free culture supernatants (CFCS) of <i>B. cereus</i> had a very strong antibacterial activity against <i>N. gonorrhoeae</i> in an in vitro antibacterial testing. Meanwhile, molecular docking revealed that antimicrobial lipopeptides from <i>B. cereus</i> (surfactin, fengycin, and iturin A) had a comparable value of binding-free energy (BFE) with the target protein receptor for <i>N. gonorrhoeae</i>, namely penicillin-binding protein (PBP) 1 and PBP2 when compared with the ceftriaxone, cefixime, and doxycycline. The molecular dynamics simulation (MDS) study revealed that the surfactin remains stable at the active site of PBP2 despite the alteration of the H-bond and hydrophobic interactions. According to this finding, surfactin has the greatest antibacterial potential against PBP2 of <i>N. gonorrhoeae</i>.
format	article
author	Nurdjannah Jane Niode Aryani Adji Jimmy Rimbing Max Tulung Mohammed Alorabi Ahmed M. El-Shehawi Rinaldi Idroes Ismail Celik Fatimawali Ahmad Akroman Adam Kuldeep Dhama Gomaa Mostafa-Hedeab Amany Abdel-Rahman Mohamed Trina Ekawati Tallei Talha Bin Emran
author_facet	Nurdjannah Jane Niode Aryani Adji Jimmy Rimbing Max Tulung Mohammed Alorabi Ahmed M. El-Shehawi Rinaldi Idroes Ismail Celik Fatimawali Ahmad Akroman Adam Kuldeep Dhama Gomaa Mostafa-Hedeab Amany Abdel-Rahman Mohamed Trina Ekawati Tallei Talha Bin Emran
author_sort	Nurdjannah Jane Niode
title	In Silico and In Vitro Evaluation of the Antimicrobial Potential of <i>Bacillus cereus</i> Isolated from <i>Apis dorsata</i> Gut against <i>Neisseria gonorrhoeae</i>
title_short	In Silico and In Vitro Evaluation of the Antimicrobial Potential of <i>Bacillus cereus</i> Isolated from <i>Apis dorsata</i> Gut against <i>Neisseria gonorrhoeae</i>
title_full	In Silico and In Vitro Evaluation of the Antimicrobial Potential of <i>Bacillus cereus</i> Isolated from <i>Apis dorsata</i> Gut against <i>Neisseria gonorrhoeae</i>
title_fullStr	In Silico and In Vitro Evaluation of the Antimicrobial Potential of <i>Bacillus cereus</i> Isolated from <i>Apis dorsata</i> Gut against <i>Neisseria gonorrhoeae</i>
title_full_unstemmed	In Silico and In Vitro Evaluation of the Antimicrobial Potential of <i>Bacillus cereus</i> Isolated from <i>Apis dorsata</i> Gut against <i>Neisseria gonorrhoeae</i>
title_sort	in silico and in vitro evaluation of the antimicrobial potential of <i>bacillus cereus</i> isolated from <i>apis dorsata</i> gut against <i>neisseria gonorrhoeae</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c3cf706a559442dd9eb7556165977105
work_keys_str_mv	AT nurdjannahjaneniode insilicoandinvitroevaluationoftheantimicrobialpotentialofibacilluscereusiisolatedfromiapisdorsataigutagainstineisseriagonorrhoeaei AT aryaniadji insilicoandinvitroevaluationoftheantimicrobialpotentialofibacilluscereusiisolatedfromiapisdorsataigutagainstineisseriagonorrhoeaei AT jimmyrimbing insilicoandinvitroevaluationoftheantimicrobialpotentialofibacilluscereusiisolatedfromiapisdorsataigutagainstineisseriagonorrhoeaei AT maxtulung insilicoandinvitroevaluationoftheantimicrobialpotentialofibacilluscereusiisolatedfromiapisdorsataigutagainstineisseriagonorrhoeaei AT mohammedalorabi insilicoandinvitroevaluationoftheantimicrobialpotentialofibacilluscereusiisolatedfromiapisdorsataigutagainstineisseriagonorrhoeaei AT ahmedmelshehawi insilicoandinvitroevaluationoftheantimicrobialpotentialofibacilluscereusiisolatedfromiapisdorsataigutagainstineisseriagonorrhoeaei AT rinaldiidroes insilicoandinvitroevaluationoftheantimicrobialpotentialofibacilluscereusiisolatedfromiapisdorsataigutagainstineisseriagonorrhoeaei AT ismailcelik insilicoandinvitroevaluationoftheantimicrobialpotentialofibacilluscereusiisolatedfromiapisdorsataigutagainstineisseriagonorrhoeaei AT fatimawali insilicoandinvitroevaluationoftheantimicrobialpotentialofibacilluscereusiisolatedfromiapisdorsataigutagainstineisseriagonorrhoeaei AT ahmadakromanadam insilicoandinvitroevaluationoftheantimicrobialpotentialofibacilluscereusiisolatedfromiapisdorsataigutagainstineisseriagonorrhoeaei AT kuldeepdhama insilicoandinvitroevaluationoftheantimicrobialpotentialofibacilluscereusiisolatedfromiapisdorsataigutagainstineisseriagonorrhoeaei AT gomaamostafahedeab insilicoandinvitroevaluationoftheantimicrobialpotentialofibacilluscereusiisolatedfromiapisdorsataigutagainstineisseriagonorrhoeaei AT amanyabdelrahmanmohamed insilicoandinvitroevaluationoftheantimicrobialpotentialofibacilluscereusiisolatedfromiapisdorsataigutagainstineisseriagonorrhoeaei AT trinaekawatitallei insilicoandinvitroevaluationoftheantimicrobialpotentialofibacilluscereusiisolatedfromiapisdorsataigutagainstineisseriagonorrhoeaei AT talhabinemran insilicoandinvitroevaluationoftheantimicrobialpotentialofibacilluscereusiisolatedfromiapisdorsataigutagainstineisseriagonorrhoeaei
_version_	1718413224652570624

In Silico and In Vitro Evaluation of the Antimicrobial Potential of <i>Bacillus cereus</i> Isolated from <i>Apis dorsata</i> Gut against <i>Neisseria gonorrhoeae</i>

Ejemplares similares