Expanding the Applicability of an Innovative Laccase TTI in Intelligent Packaging by Adding an Enzyme Inhibitor to Change Its Coloration Kinetics

Enzymatic time–temperature indicators (TTIs) usually suffer from instability and inefficiency in practical use as food quality indicator during storage. The aim of this study was to address the aforementioned problem by immobilizing laccase on electrospun chitosan fibers to increase the stability an...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cheng-Xuan Lin, Hao-Hsin Hsu, Yu-Hsuan Chang, Shih-Hsin Chen, Shih-Bin Lin, Shyi-Neng Lou, Hui-Huang Chen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	time–temperature indicator laccase electrospinning sodium azide kinetic intelligent packaging Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/c3dc4c69b0174610a3d9c09b51d2ae46
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c3dc4c69b0174610a3d9c09b51d2ae46
record_format	dspace
spelling	oai:doaj.org-article:c3dc4c69b0174610a3d9c09b51d2ae462021-11-11T18:42:33ZExpanding the Applicability of an Innovative Laccase TTI in Intelligent Packaging by Adding an Enzyme Inhibitor to Change Its Coloration Kinetics10.3390/polym132136462073-4360https://doaj.org/article/c3dc4c69b0174610a3d9c09b51d2ae462021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/21/3646https://doaj.org/toc/2073-4360Enzymatic time–temperature indicators (TTIs) usually suffer from instability and inefficiency in practical use as food quality indicator during storage. The aim of this study was to address the aforementioned problem by immobilizing laccase on electrospun chitosan fibers to increase the stability and minimize the usage of laccase. The addition of NaN<sub>3</sub>, as and enzyme inhibitor, was intended to extend this laccase TTI coloration rate and activation energy (<i>Ea</i>) range, so as to expand the application range of TTIs for evaluating changes in the quality of foods during storage. A two-component time–temperature indicator was prepared by immobilizing laccase on electrospun chitosan fibers as a TTI film, and by using guaiacol solution as a coloration substrate. The color difference of the innovative laccase TTI was discovered to be <3, and visually indistinguishable when OD<sub>500</sub> reached 3.2; the response reaction time was regarded as the TTI’s coloration endpoint. Enzyme immobilization and the addition of NaN<sub>3</sub> increased coloration <i>Km</i> and reduced coloration <i>Vmax</i>. The coloration <i>Vmax</i> decreased to 64% when 0.1 mM NaN<sub>3</sub> was added to the TTI, which exhibited noncompetitive inhibition and a slower coloration rate. Coloration hysteresis appeared in the TTI with NaN<sub>3</sub>, particularly at low temperatures. For TTI coloration, the <i>Ea</i> increased to 29.92–66.39 kJ/mol when 15–25 μg/cm<sup>2</sup> of laccase was immobilized, and the endpoint increased to 11.0–199.5 h when 0–0.10 mM NaN<sub>3</sub> was added. These modifications expanded the applicability of laccase TTIs in intelligent food packaging.Cheng-Xuan LinHao-Hsin HsuYu-Hsuan ChangShih-Hsin ChenShih-Bin LinShyi-Neng LouHui-Huang ChenMDPI AGarticletime–temperature indicatorlaccaseelectrospinningsodium azidekineticintelligent packagingOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 3646, p 3646 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	time–temperature indicator laccase electrospinning sodium azide kinetic intelligent packaging Organic chemistry QD241-441
spellingShingle	time–temperature indicator laccase electrospinning sodium azide kinetic intelligent packaging Organic chemistry QD241-441 Cheng-Xuan Lin Hao-Hsin Hsu Yu-Hsuan Chang Shih-Hsin Chen Shih-Bin Lin Shyi-Neng Lou Hui-Huang Chen Expanding the Applicability of an Innovative Laccase TTI in Intelligent Packaging by Adding an Enzyme Inhibitor to Change Its Coloration Kinetics
description	Enzymatic time–temperature indicators (TTIs) usually suffer from instability and inefficiency in practical use as food quality indicator during storage. The aim of this study was to address the aforementioned problem by immobilizing laccase on electrospun chitosan fibers to increase the stability and minimize the usage of laccase. The addition of NaN<sub>3</sub>, as and enzyme inhibitor, was intended to extend this laccase TTI coloration rate and activation energy (<i>Ea</i>) range, so as to expand the application range of TTIs for evaluating changes in the quality of foods during storage. A two-component time–temperature indicator was prepared by immobilizing laccase on electrospun chitosan fibers as a TTI film, and by using guaiacol solution as a coloration substrate. The color difference of the innovative laccase TTI was discovered to be <3, and visually indistinguishable when OD<sub>500</sub> reached 3.2; the response reaction time was regarded as the TTI’s coloration endpoint. Enzyme immobilization and the addition of NaN<sub>3</sub> increased coloration <i>Km</i> and reduced coloration <i>Vmax</i>. The coloration <i>Vmax</i> decreased to 64% when 0.1 mM NaN<sub>3</sub> was added to the TTI, which exhibited noncompetitive inhibition and a slower coloration rate. Coloration hysteresis appeared in the TTI with NaN<sub>3</sub>, particularly at low temperatures. For TTI coloration, the <i>Ea</i> increased to 29.92–66.39 kJ/mol when 15–25 μg/cm<sup>2</sup> of laccase was immobilized, and the endpoint increased to 11.0–199.5 h when 0–0.10 mM NaN<sub>3</sub> was added. These modifications expanded the applicability of laccase TTIs in intelligent food packaging.
format	article
author	Cheng-Xuan Lin Hao-Hsin Hsu Yu-Hsuan Chang Shih-Hsin Chen Shih-Bin Lin Shyi-Neng Lou Hui-Huang Chen
author_facet	Cheng-Xuan Lin Hao-Hsin Hsu Yu-Hsuan Chang Shih-Hsin Chen Shih-Bin Lin Shyi-Neng Lou Hui-Huang Chen
author_sort	Cheng-Xuan Lin
title	Expanding the Applicability of an Innovative Laccase TTI in Intelligent Packaging by Adding an Enzyme Inhibitor to Change Its Coloration Kinetics
title_short	Expanding the Applicability of an Innovative Laccase TTI in Intelligent Packaging by Adding an Enzyme Inhibitor to Change Its Coloration Kinetics
title_full	Expanding the Applicability of an Innovative Laccase TTI in Intelligent Packaging by Adding an Enzyme Inhibitor to Change Its Coloration Kinetics
title_fullStr	Expanding the Applicability of an Innovative Laccase TTI in Intelligent Packaging by Adding an Enzyme Inhibitor to Change Its Coloration Kinetics
title_full_unstemmed	Expanding the Applicability of an Innovative Laccase TTI in Intelligent Packaging by Adding an Enzyme Inhibitor to Change Its Coloration Kinetics
title_sort	expanding the applicability of an innovative laccase tti in intelligent packaging by adding an enzyme inhibitor to change its coloration kinetics
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c3dc4c69b0174610a3d9c09b51d2ae46
work_keys_str_mv	AT chengxuanlin expandingtheapplicabilityofaninnovativelaccasettiinintelligentpackagingbyaddinganenzymeinhibitortochangeitscolorationkinetics AT haohsinhsu expandingtheapplicabilityofaninnovativelaccasettiinintelligentpackagingbyaddinganenzymeinhibitortochangeitscolorationkinetics AT yuhsuanchang expandingtheapplicabilityofaninnovativelaccasettiinintelligentpackagingbyaddinganenzymeinhibitortochangeitscolorationkinetics AT shihhsinchen expandingtheapplicabilityofaninnovativelaccasettiinintelligentpackagingbyaddinganenzymeinhibitortochangeitscolorationkinetics AT shihbinlin expandingtheapplicabilityofaninnovativelaccasettiinintelligentpackagingbyaddinganenzymeinhibitortochangeitscolorationkinetics AT shyinenglou expandingtheapplicabilityofaninnovativelaccasettiinintelligentpackagingbyaddinganenzymeinhibitortochangeitscolorationkinetics AT huihuangchen expandingtheapplicabilityofaninnovativelaccasettiinintelligentpackagingbyaddinganenzymeinhibitortochangeitscolorationkinetics
_version_	1718431786354081792

Expanding the Applicability of an Innovative Laccase TTI in Intelligent Packaging by Adding an Enzyme Inhibitor to Change Its Coloration Kinetics

Ejemplares similares