Eight-year longitudinal study of whole blood gene expression profiles in individuals undergoing long-term medical follow-up

Abstract Blood circulates throughout the body via the peripheral tissues, contributes to host homeostasis and maintains normal physiological functions, in addition to responding to lesions. Previously, we revealed that gene expression analysis of peripheral blood cells is a useful approach for asses...

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Autores principales:	Yoshio Sakai, Alessandro Nasti, Yumie Takeshita, Miki Okumura, Shinji Kitajima, Masao Honda, Takashi Wada, Seiji Nakamura, Toshinari Takamura, Takuro Tamura, Kenichi Matsubara, Shuichi Kaneko
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c3e2e6d9ee3c4d86882da2cb62e36557
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spelling	oai:doaj.org-article:c3e2e6d9ee3c4d86882da2cb62e365572021-12-02T18:51:46ZEight-year longitudinal study of whole blood gene expression profiles in individuals undergoing long-term medical follow-up10.1038/s41598-021-96078-02045-2322https://doaj.org/article/c3e2e6d9ee3c4d86882da2cb62e365572021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96078-0https://doaj.org/toc/2045-2322Abstract Blood circulates throughout the body via the peripheral tissues, contributes to host homeostasis and maintains normal physiological functions, in addition to responding to lesions. Previously, we revealed that gene expression analysis of peripheral blood cells is a useful approach for assessing diseases such as diabetes mellitus and cancer because the altered gene expression profiles of peripheral blood cells can reflect the presence and state of diseases. However, no chronological assessment of whole gene expression profiles has been conducted. In the present study, we collected whole blood RNA from 61 individuals (average age at registration, 50 years) every 4 years for 8 years and analyzed gene expression profiles using a complementary DNA microarray to examine whether these profiles were stable or changed over time. We found that the genes with very stable expression were related mostly to immune system pathways, including antigen cell presentation and interferon-related signaling. Genes whose expression was altered over the 8-year study period were principally involved in cellular machinery pathways, including development, signal transduction, cell cycle, apoptosis, and survival. Thus, this chronological examination study showed that the gene expression profiles of whole blood can reveal unmanifested physiological changes.Yoshio SakaiAlessandro NastiYumie TakeshitaMiki OkumuraShinji KitajimaMasao HondaTakashi WadaSeiji NakamuraToshinari TakamuraTakuro TamuraKenichi MatsubaraShuichi KanekoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yoshio Sakai Alessandro Nasti Yumie Takeshita Miki Okumura Shinji Kitajima Masao Honda Takashi Wada Seiji Nakamura Toshinari Takamura Takuro Tamura Kenichi Matsubara Shuichi Kaneko Eight-year longitudinal study of whole blood gene expression profiles in individuals undergoing long-term medical follow-up
description	Abstract Blood circulates throughout the body via the peripheral tissues, contributes to host homeostasis and maintains normal physiological functions, in addition to responding to lesions. Previously, we revealed that gene expression analysis of peripheral blood cells is a useful approach for assessing diseases such as diabetes mellitus and cancer because the altered gene expression profiles of peripheral blood cells can reflect the presence and state of diseases. However, no chronological assessment of whole gene expression profiles has been conducted. In the present study, we collected whole blood RNA from 61 individuals (average age at registration, 50 years) every 4 years for 8 years and analyzed gene expression profiles using a complementary DNA microarray to examine whether these profiles were stable or changed over time. We found that the genes with very stable expression were related mostly to immune system pathways, including antigen cell presentation and interferon-related signaling. Genes whose expression was altered over the 8-year study period were principally involved in cellular machinery pathways, including development, signal transduction, cell cycle, apoptosis, and survival. Thus, this chronological examination study showed that the gene expression profiles of whole blood can reveal unmanifested physiological changes.
format	article
author	Yoshio Sakai Alessandro Nasti Yumie Takeshita Miki Okumura Shinji Kitajima Masao Honda Takashi Wada Seiji Nakamura Toshinari Takamura Takuro Tamura Kenichi Matsubara Shuichi Kaneko
author_facet	Yoshio Sakai Alessandro Nasti Yumie Takeshita Miki Okumura Shinji Kitajima Masao Honda Takashi Wada Seiji Nakamura Toshinari Takamura Takuro Tamura Kenichi Matsubara Shuichi Kaneko
author_sort	Yoshio Sakai
title	Eight-year longitudinal study of whole blood gene expression profiles in individuals undergoing long-term medical follow-up
title_short	Eight-year longitudinal study of whole blood gene expression profiles in individuals undergoing long-term medical follow-up
title_full	Eight-year longitudinal study of whole blood gene expression profiles in individuals undergoing long-term medical follow-up
title_fullStr	Eight-year longitudinal study of whole blood gene expression profiles in individuals undergoing long-term medical follow-up
title_full_unstemmed	Eight-year longitudinal study of whole blood gene expression profiles in individuals undergoing long-term medical follow-up
title_sort	eight-year longitudinal study of whole blood gene expression profiles in individuals undergoing long-term medical follow-up
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c3e2e6d9ee3c4d86882da2cb62e36557
work_keys_str_mv	AT yoshiosakai eightyearlongitudinalstudyofwholebloodgeneexpressionprofilesinindividualsundergoinglongtermmedicalfollowup AT alessandronasti eightyearlongitudinalstudyofwholebloodgeneexpressionprofilesinindividualsundergoinglongtermmedicalfollowup AT yumietakeshita eightyearlongitudinalstudyofwholebloodgeneexpressionprofilesinindividualsundergoinglongtermmedicalfollowup AT mikiokumura eightyearlongitudinalstudyofwholebloodgeneexpressionprofilesinindividualsundergoinglongtermmedicalfollowup AT shinjikitajima eightyearlongitudinalstudyofwholebloodgeneexpressionprofilesinindividualsundergoinglongtermmedicalfollowup AT masaohonda eightyearlongitudinalstudyofwholebloodgeneexpressionprofilesinindividualsundergoinglongtermmedicalfollowup AT takashiwada eightyearlongitudinalstudyofwholebloodgeneexpressionprofilesinindividualsundergoinglongtermmedicalfollowup AT seijinakamura eightyearlongitudinalstudyofwholebloodgeneexpressionprofilesinindividualsundergoinglongtermmedicalfollowup AT toshinaritakamura eightyearlongitudinalstudyofwholebloodgeneexpressionprofilesinindividualsundergoinglongtermmedicalfollowup AT takurotamura eightyearlongitudinalstudyofwholebloodgeneexpressionprofilesinindividualsundergoinglongtermmedicalfollowup AT kenichimatsubara eightyearlongitudinalstudyofwholebloodgeneexpressionprofilesinindividualsundergoinglongtermmedicalfollowup AT shuichikaneko eightyearlongitudinalstudyofwholebloodgeneexpressionprofilesinindividualsundergoinglongtermmedicalfollowup
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Eight-year longitudinal study of whole blood gene expression profiles in individuals undergoing long-term medical follow-up

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