Phylogenetic and functional metagenomic profiling for assessing microbial biodiversity in environmental monitoring.

Decisions guiding environmental management need to be based on a broad and comprehensive understanding of the biodiversity and functional capability within ecosystems. Microbes are of particular importance since they drive biogeochemical cycles, being both producers and decomposers. Their quick and...

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Autores principales:	Veljo Kisand, Angelica Valente, Armin Lahm, Gerard Tanet, Teresa Lettieri
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c3e88459e00d43ad8bdcc16addd2809e
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spelling	oai:doaj.org-article:c3e88459e00d43ad8bdcc16addd2809e2021-11-18T07:07:33ZPhylogenetic and functional metagenomic profiling for assessing microbial biodiversity in environmental monitoring.1932-620310.1371/journal.pone.0043630https://doaj.org/article/c3e88459e00d43ad8bdcc16addd2809e2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22952724/?tool=EBIhttps://doaj.org/toc/1932-6203Decisions guiding environmental management need to be based on a broad and comprehensive understanding of the biodiversity and functional capability within ecosystems. Microbes are of particular importance since they drive biogeochemical cycles, being both producers and decomposers. Their quick and direct responses to changes in environmental conditions modulate the ecosystem accordingly, thus providing a sensitive readout. Here we have used direct sequencing of total DNA from water samples to compare the microbial communities of two distinct coastal regions exposed to different anthropogenic pressures: the highly polluted Port of Genoa and the protected area of Montecristo Island in the Mediterranean Sea. Analysis of the metagenomes revealed significant differences in both microbial diversity and abundance between the two areas, reflecting their distinct ecological habitats and anthropogenic stress conditions. Our results indicate that the combination of next generation sequencing (NGS) technologies and bioinformatics tools presents a new approach to monitor the diversity and the ecological status of aquatic ecosystems. Integration of metagenomics into environmental monitoring campaigns should enable the impact of the anthropogenic pressure on microbial biodiversity in various ecosystems to be better assessed and also predicted.Veljo KisandAngelica ValenteArmin LahmGerard TanetTeresa LettieriPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e43630 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Veljo Kisand Angelica Valente Armin Lahm Gerard Tanet Teresa Lettieri Phylogenetic and functional metagenomic profiling for assessing microbial biodiversity in environmental monitoring.
description	Decisions guiding environmental management need to be based on a broad and comprehensive understanding of the biodiversity and functional capability within ecosystems. Microbes are of particular importance since they drive biogeochemical cycles, being both producers and decomposers. Their quick and direct responses to changes in environmental conditions modulate the ecosystem accordingly, thus providing a sensitive readout. Here we have used direct sequencing of total DNA from water samples to compare the microbial communities of two distinct coastal regions exposed to different anthropogenic pressures: the highly polluted Port of Genoa and the protected area of Montecristo Island in the Mediterranean Sea. Analysis of the metagenomes revealed significant differences in both microbial diversity and abundance between the two areas, reflecting their distinct ecological habitats and anthropogenic stress conditions. Our results indicate that the combination of next generation sequencing (NGS) technologies and bioinformatics tools presents a new approach to monitor the diversity and the ecological status of aquatic ecosystems. Integration of metagenomics into environmental monitoring campaigns should enable the impact of the anthropogenic pressure on microbial biodiversity in various ecosystems to be better assessed and also predicted.
format	article
author	Veljo Kisand Angelica Valente Armin Lahm Gerard Tanet Teresa Lettieri
author_facet	Veljo Kisand Angelica Valente Armin Lahm Gerard Tanet Teresa Lettieri
author_sort	Veljo Kisand
title	Phylogenetic and functional metagenomic profiling for assessing microbial biodiversity in environmental monitoring.
title_short	Phylogenetic and functional metagenomic profiling for assessing microbial biodiversity in environmental monitoring.
title_full	Phylogenetic and functional metagenomic profiling for assessing microbial biodiversity in environmental monitoring.
title_fullStr	Phylogenetic and functional metagenomic profiling for assessing microbial biodiversity in environmental monitoring.
title_full_unstemmed	Phylogenetic and functional metagenomic profiling for assessing microbial biodiversity in environmental monitoring.
title_sort	phylogenetic and functional metagenomic profiling for assessing microbial biodiversity in environmental monitoring.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/c3e88459e00d43ad8bdcc16addd2809e
work_keys_str_mv	AT veljokisand phylogeneticandfunctionalmetagenomicprofilingforassessingmicrobialbiodiversityinenvironmentalmonitoring AT angelicavalente phylogeneticandfunctionalmetagenomicprofilingforassessingmicrobialbiodiversityinenvironmentalmonitoring AT arminlahm phylogeneticandfunctionalmetagenomicprofilingforassessingmicrobialbiodiversityinenvironmentalmonitoring AT gerardtanet phylogeneticandfunctionalmetagenomicprofilingforassessingmicrobialbiodiversityinenvironmentalmonitoring AT teresalettieri phylogeneticandfunctionalmetagenomicprofilingforassessingmicrobialbiodiversityinenvironmentalmonitoring
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Phylogenetic and functional metagenomic profiling for assessing microbial biodiversity in environmental monitoring.

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