Differences in Morphokinetic Parameters and Incidence of Multinucleations in Human Embryos of Genetically Normal, Abnormal and Euploid Embryos Leading to Clinical Pregnancy

The selection of the best embryo for embryo transfer (ET) is one of the most important steps in IVF (in vitro fertilisation) treatment. Preimplantation genetic testing (PGT) is an invasive method that can greatly facilitate the decision about the best embryo. An alternative way to select the embryo...

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Autores principales:	Katerina Tvrdonova, Silvie Belaskova, Tatana Rumpikova, Alice Malenovska, David Rumpik, Alena Myslivcova Fucikova, Frantisek Malir
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	time-lapse monitoring morphokinetics multinucleation embryo development preimplantation genetic testing Medicine R
Acceso en línea:	https://doaj.org/article/c3ea12dd027444c8bee1daae698f48bb
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spelling	oai:doaj.org-article:c3ea12dd027444c8bee1daae698f48bb2021-11-11T17:47:10ZDifferences in Morphokinetic Parameters and Incidence of Multinucleations in Human Embryos of Genetically Normal, Abnormal and Euploid Embryos Leading to Clinical Pregnancy10.3390/jcm102151732077-0383https://doaj.org/article/c3ea12dd027444c8bee1daae698f48bb2021-11-01T00:00:00Zhttps://www.mdpi.com/2077-0383/10/21/5173https://doaj.org/toc/2077-0383The selection of the best embryo for embryo transfer (ET) is one of the most important steps in IVF (in vitro fertilisation) treatment. Preimplantation genetic testing (PGT) is an invasive method that can greatly facilitate the decision about the best embryo. An alternative way to select the embryo with the greatest implantation potential is by cultivation in a time-lapse system, which can offer several predictive factors. Non-invasive time-lapse monitoring can be used to select quality embryos with high implantation potential under stable culture conditions. The embryo for ET can then be selected based on the determined morphokinetic parameters and morphological features, which according to our results predict a higher implantation potential. This study included a total of 1027 morphologically high-quality embryos (552 normal and 475 abnormal PGT-tested embryos) from 296 patients (01/2016–06/2021). All embryos were cultivated in a time-lapse incubator and PGT biopsy of trophectoderm cells on D5 or D6 was performed. Significant differences were found in the morphological parameters cc2, t5 and tSB and the occurrence of multinucleations in the stage of two-cell and four-cell embryos between the group of genetically normal embryos and abnormal embryos. At the same time, significant differences in the morphological parameters cc2, t5 and tSB and the occurrence of multinucleations in the two-cell and four-cell embryo stage were found between the group of genetically normal embryos that led to clinical pregnancy after ET and the group of abnormal embryos. From the morphokinetic data found in the PGT-A group of normal embryos leading to clinical pregnancy, time intervals were determined based on statistical analysis, which should predict embryos with high implantation potential. Out of a total of 218 euploid embryos, which were transferred into the uterus after thawing (single frozen embryo transfer), clinical pregnancy was confirmed in 119 embryos (54.6%). Our results show that according to the morphokinetic parameters (cc2, t5, tSB) and the occurrence of multinucleations during the first two cell divisions, the best euploid embryo for ET can be selected with high probability.Katerina TvrdonovaSilvie BelaskovaTatana RumpikovaAlice MalenovskaDavid RumpikAlena Myslivcova FucikovaFrantisek MalirMDPI AGarticletime-lapse monitoringmorphokineticsmultinucleationembryo developmentpreimplantation genetic testingMedicineRENJournal of Clinical Medicine, Vol 10, Iss 5173, p 5173 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	time-lapse monitoring morphokinetics multinucleation embryo development preimplantation genetic testing Medicine R
spellingShingle	time-lapse monitoring morphokinetics multinucleation embryo development preimplantation genetic testing Medicine R Katerina Tvrdonova Silvie Belaskova Tatana Rumpikova Alice Malenovska David Rumpik Alena Myslivcova Fucikova Frantisek Malir Differences in Morphokinetic Parameters and Incidence of Multinucleations in Human Embryos of Genetically Normal, Abnormal and Euploid Embryos Leading to Clinical Pregnancy
description	The selection of the best embryo for embryo transfer (ET) is one of the most important steps in IVF (in vitro fertilisation) treatment. Preimplantation genetic testing (PGT) is an invasive method that can greatly facilitate the decision about the best embryo. An alternative way to select the embryo with the greatest implantation potential is by cultivation in a time-lapse system, which can offer several predictive factors. Non-invasive time-lapse monitoring can be used to select quality embryos with high implantation potential under stable culture conditions. The embryo for ET can then be selected based on the determined morphokinetic parameters and morphological features, which according to our results predict a higher implantation potential. This study included a total of 1027 morphologically high-quality embryos (552 normal and 475 abnormal PGT-tested embryos) from 296 patients (01/2016–06/2021). All embryos were cultivated in a time-lapse incubator and PGT biopsy of trophectoderm cells on D5 or D6 was performed. Significant differences were found in the morphological parameters cc2, t5 and tSB and the occurrence of multinucleations in the stage of two-cell and four-cell embryos between the group of genetically normal embryos and abnormal embryos. At the same time, significant differences in the morphological parameters cc2, t5 and tSB and the occurrence of multinucleations in the two-cell and four-cell embryo stage were found between the group of genetically normal embryos that led to clinical pregnancy after ET and the group of abnormal embryos. From the morphokinetic data found in the PGT-A group of normal embryos leading to clinical pregnancy, time intervals were determined based on statistical analysis, which should predict embryos with high implantation potential. Out of a total of 218 euploid embryos, which were transferred into the uterus after thawing (single frozen embryo transfer), clinical pregnancy was confirmed in 119 embryos (54.6%). Our results show that according to the morphokinetic parameters (cc2, t5, tSB) and the occurrence of multinucleations during the first two cell divisions, the best euploid embryo for ET can be selected with high probability.
format	article
author	Katerina Tvrdonova Silvie Belaskova Tatana Rumpikova Alice Malenovska David Rumpik Alena Myslivcova Fucikova Frantisek Malir
author_facet	Katerina Tvrdonova Silvie Belaskova Tatana Rumpikova Alice Malenovska David Rumpik Alena Myslivcova Fucikova Frantisek Malir
author_sort	Katerina Tvrdonova
title	Differences in Morphokinetic Parameters and Incidence of Multinucleations in Human Embryos of Genetically Normal, Abnormal and Euploid Embryos Leading to Clinical Pregnancy
title_short	Differences in Morphokinetic Parameters and Incidence of Multinucleations in Human Embryos of Genetically Normal, Abnormal and Euploid Embryos Leading to Clinical Pregnancy
title_full	Differences in Morphokinetic Parameters and Incidence of Multinucleations in Human Embryos of Genetically Normal, Abnormal and Euploid Embryos Leading to Clinical Pregnancy
title_fullStr	Differences in Morphokinetic Parameters and Incidence of Multinucleations in Human Embryos of Genetically Normal, Abnormal and Euploid Embryos Leading to Clinical Pregnancy
title_full_unstemmed	Differences in Morphokinetic Parameters and Incidence of Multinucleations in Human Embryos of Genetically Normal, Abnormal and Euploid Embryos Leading to Clinical Pregnancy
title_sort	differences in morphokinetic parameters and incidence of multinucleations in human embryos of genetically normal, abnormal and euploid embryos leading to clinical pregnancy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c3ea12dd027444c8bee1daae698f48bb
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Differences in Morphokinetic Parameters and Incidence of Multinucleations in Human Embryos of Genetically Normal, Abnormal and Euploid Embryos Leading to Clinical Pregnancy

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