Larval cryopreservation as new management tool for threatened clam fisheries

Abstract Cryopreservation is the only reliable method for long-term storage of biological material that guarantees genetic stability. This technique can be extremely useful for the conservation of endangered species and restock natural populations for declining species. Many factors have negatively...

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Autores principales:	P. Heres, J. Troncoso, E. Paredes
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c3f364339e5a4573815a74d793538814
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spelling	oai:doaj.org-article:c3f364339e5a4573815a74d7935388142021-12-02T18:47:08ZLarval cryopreservation as new management tool for threatened clam fisheries10.1038/s41598-021-94197-22045-2322https://doaj.org/article/c3f364339e5a4573815a74d7935388142021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94197-2https://doaj.org/toc/2045-2322Abstract Cryopreservation is the only reliable method for long-term storage of biological material that guarantees genetic stability. This technique can be extremely useful for the conservation of endangered species and restock natural populations for declining species. Many factors have negatively affected the populations of high economical value shellfish in Spain and, as a result, many are declining or threatened nowadays. This study was focused on early-life stages of Venerupis corrugata, Ruditapes decussatus and Ruditapes philippinarum to develop successful protocols to enhance the conservation effort and sustainable shellfishery resources. Firstly, common cryoprotecting agents (CPAs) were tested to select the suitable permeable CPA attending to toxicity. Cryopreservation success using different combinations of CPA solutions, increasing equilibrium times and larval stages was evaluated attending to survival and shell growth at 2 days post-thawing. Older clam development stages were more tolerant to CPA toxicity, being ethylene-glycol (EG) and Propylene-glycol (PG) the least toxic CPAs. CPA solution containing EG yielded the highest post-thawing survival rate and the increase of equilibration time was not beneficial for clam larvae. Cryopreservation of trochophores yielded around 50% survivorship, whereas over 80% of cryopreserved D-larvae were able to recover after thawing.P. HeresJ. TroncosoE. ParedesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q P. Heres J. Troncoso E. Paredes Larval cryopreservation as new management tool for threatened clam fisheries
description	Abstract Cryopreservation is the only reliable method for long-term storage of biological material that guarantees genetic stability. This technique can be extremely useful for the conservation of endangered species and restock natural populations for declining species. Many factors have negatively affected the populations of high economical value shellfish in Spain and, as a result, many are declining or threatened nowadays. This study was focused on early-life stages of Venerupis corrugata, Ruditapes decussatus and Ruditapes philippinarum to develop successful protocols to enhance the conservation effort and sustainable shellfishery resources. Firstly, common cryoprotecting agents (CPAs) were tested to select the suitable permeable CPA attending to toxicity. Cryopreservation success using different combinations of CPA solutions, increasing equilibrium times and larval stages was evaluated attending to survival and shell growth at 2 days post-thawing. Older clam development stages were more tolerant to CPA toxicity, being ethylene-glycol (EG) and Propylene-glycol (PG) the least toxic CPAs. CPA solution containing EG yielded the highest post-thawing survival rate and the increase of equilibration time was not beneficial for clam larvae. Cryopreservation of trochophores yielded around 50% survivorship, whereas over 80% of cryopreserved D-larvae were able to recover after thawing.
format	article
author	P. Heres J. Troncoso E. Paredes
author_facet	P. Heres J. Troncoso E. Paredes
author_sort	P. Heres
title	Larval cryopreservation as new management tool for threatened clam fisheries
title_short	Larval cryopreservation as new management tool for threatened clam fisheries
title_full	Larval cryopreservation as new management tool for threatened clam fisheries
title_fullStr	Larval cryopreservation as new management tool for threatened clam fisheries
title_full_unstemmed	Larval cryopreservation as new management tool for threatened clam fisheries
title_sort	larval cryopreservation as new management tool for threatened clam fisheries
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c3f364339e5a4573815a74d793538814
work_keys_str_mv	AT pheres larvalcryopreservationasnewmanagementtoolforthreatenedclamfisheries AT jtroncoso larvalcryopreservationasnewmanagementtoolforthreatenedclamfisheries AT eparedes larvalcryopreservationasnewmanagementtoolforthreatenedclamfisheries
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Larval cryopreservation as new management tool for threatened clam fisheries

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