Increase in gut microbiota after immune suppression in baculovirus-infected larvae.

Spodoptera exigua microarray was used to determine genes differentially expressed in S. exigua cells challenged with the species-specific baculovirus SeMNPV as well as with a generalist baculovirus, AcMNPV. Microarray results revealed that, in contrast to the host transcriptional shut-off that is ex...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Agata K Jakubowska, Heiko Vogel, Salvador Herrero
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c3f6ad289c9549b1944c048fcf7d99e5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c3f6ad289c9549b1944c048fcf7d99e5
record_format	dspace
spelling	oai:doaj.org-article:c3f6ad289c9549b1944c048fcf7d99e52021-11-18T06:05:36ZIncrease in gut microbiota after immune suppression in baculovirus-infected larvae.1553-73661553-737410.1371/journal.ppat.1003379https://doaj.org/article/c3f6ad289c9549b1944c048fcf7d99e52013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23717206/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Spodoptera exigua microarray was used to determine genes differentially expressed in S. exigua cells challenged with the species-specific baculovirus SeMNPV as well as with a generalist baculovirus, AcMNPV. Microarray results revealed that, in contrast to the host transcriptional shut-off that is expected during baculovirus infection, S. exigua cells showed a balanced number of up- and down-regulated genes during the first 36 hours following the infection. Many immune-related genes, including pattern recognition proteins, genes involved in signalling and immune pathways as well as immune effectors and genes coding for proteins involved in the melanization cascade were found to be down-regulated after baculovirus infection. The down-regulation of immune-related genes was confirmed in the larval gut. The expression of immune-related genes in the gut is known to affect the status of gut microorganisms, many of which are responsible for growth and development functions. We therefore asked whether the down-regulation that occurs after baculovirus infection affects the amount of gut microbiota. An increase in the gut bacterial load was observed and we hypothesize this to be as a consequence of viral infection. Subsequent experiments on virus performance in the presence and absence of gut microbiota revealed that gut bacteria enhanced baculovirus virulence, pathogenicity and dispersion. We discuss the host immune response processes and pathways affected by baculoviruses, as well as the role of gut microbiota in viral infection.Agata K JakubowskaHeiko VogelSalvador HerreroPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 9, Iss 5, p e1003379 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Agata K Jakubowska Heiko Vogel Salvador Herrero Increase in gut microbiota after immune suppression in baculovirus-infected larvae.
description	Spodoptera exigua microarray was used to determine genes differentially expressed in S. exigua cells challenged with the species-specific baculovirus SeMNPV as well as with a generalist baculovirus, AcMNPV. Microarray results revealed that, in contrast to the host transcriptional shut-off that is expected during baculovirus infection, S. exigua cells showed a balanced number of up- and down-regulated genes during the first 36 hours following the infection. Many immune-related genes, including pattern recognition proteins, genes involved in signalling and immune pathways as well as immune effectors and genes coding for proteins involved in the melanization cascade were found to be down-regulated after baculovirus infection. The down-regulation of immune-related genes was confirmed in the larval gut. The expression of immune-related genes in the gut is known to affect the status of gut microorganisms, many of which are responsible for growth and development functions. We therefore asked whether the down-regulation that occurs after baculovirus infection affects the amount of gut microbiota. An increase in the gut bacterial load was observed and we hypothesize this to be as a consequence of viral infection. Subsequent experiments on virus performance in the presence and absence of gut microbiota revealed that gut bacteria enhanced baculovirus virulence, pathogenicity and dispersion. We discuss the host immune response processes and pathways affected by baculoviruses, as well as the role of gut microbiota in viral infection.
format	article
author	Agata K Jakubowska Heiko Vogel Salvador Herrero
author_facet	Agata K Jakubowska Heiko Vogel Salvador Herrero
author_sort	Agata K Jakubowska
title	Increase in gut microbiota after immune suppression in baculovirus-infected larvae.
title_short	Increase in gut microbiota after immune suppression in baculovirus-infected larvae.
title_full	Increase in gut microbiota after immune suppression in baculovirus-infected larvae.
title_fullStr	Increase in gut microbiota after immune suppression in baculovirus-infected larvae.
title_full_unstemmed	Increase in gut microbiota after immune suppression in baculovirus-infected larvae.
title_sort	increase in gut microbiota after immune suppression in baculovirus-infected larvae.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/c3f6ad289c9549b1944c048fcf7d99e5
work_keys_str_mv	AT agatakjakubowska increaseingutmicrobiotaafterimmunesuppressioninbaculovirusinfectedlarvae AT heikovogel increaseingutmicrobiotaafterimmunesuppressioninbaculovirusinfectedlarvae AT salvadorherrero increaseingutmicrobiotaafterimmunesuppressioninbaculovirusinfectedlarvae
_version_	1718424630725705728

Increase in gut microbiota after immune suppression in baculovirus-infected larvae.

Ejemplares similares