Graphene Oxide Negatively Regulates Cell Cycle in Embryonic Fibroblast Cells

Ehsan Hashemi,1– 3 Omid Akhavan,4 Mehdi Shamsara,1,2 Saeid Ansari Majd,2 Mohammad Hossein Sanati,5 Morteza Daliri Joupari,1,2 Abbas Farmany6 1Animal Biotechnology Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran; 2National Research Center for Transgenic...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hashemi E, Akhavan O, Shamsara M, Ansari Majd S, Sanati MH, Daliri Joupari M, Farmany A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	apoptosis cell cycle fibroblast cell graphene tp53 gene Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c3faad23619a48518718450e5cae03d3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c3faad23619a48518718450e5cae03d3
record_format	dspace
spelling	oai:doaj.org-article:c3faad23619a48518718450e5cae03d32021-12-02T10:31:12ZGraphene Oxide Negatively Regulates Cell Cycle in Embryonic Fibroblast Cells1178-2013https://doaj.org/article/c3faad23619a48518718450e5cae03d32020-08-01T00:00:00Zhttps://www.dovepress.com/graphene-oxide-negatively-regulates-cell-cycle-in-embryonic-fibroblast-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ehsan Hashemi,1– 3 Omid Akhavan,4 Mehdi Shamsara,1,2 Saeid Ansari Majd,2 Mohammad Hossein Sanati,5 Morteza Daliri Joupari,1,2 Abbas Farmany6 1Animal Biotechnology Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran; 2National Research Center for Transgenic Mouse, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran; 3Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran; 4Department of Physics, Sharif University of Technology, Tehran, Iran; 5Medical Genetics Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran; 6Dental Research Center, Hamadan University of Medical Sciences, Hamadan, IranCorrespondence: Morteza Daliri Joupari; Abbas Farmany Tel +98 21 4458-0340Fax +98 21 44580393Email daliri@nigeb.ac.ir; a.farmany@ut.ac.irBackground: Unique properties of graphene and its derivatives make them attractive in the field of nanomedicine. However, the mass application of graphene might lead to side effects, which has not been properly addressed in previous studies, especially with regard to its effect on the cell cycle.Methods: The effect of two concentrations (100 and 200 μg/mL) of nano- and microsized graphene oxide (nGO and mGO) on apoptosis, cell cycle, and ROS generation was studied. The effect of both sizes on viability and genotoxicity of the embryonic fibroblast cell cycle was evaluated. MTT and flow cytometry were applied to evaluate the effects of graphene oxide (GO) nanosheets on viability of cells. Apoptosis and cell cycle were analyzed by flow cytometry.Results: The results of this study showed that GO disturbed the cell cycle and nGO impaired cell viability by inducing cell apoptosis. Interestingly, both nGO and mGO blocked the cell cycle in the S phase, which is a critical phase of the cell cycle. Upregulation of TP53-gene transcripts was also detected in both nGO- and mGO-treated cells compared to the control, especially at 200 μg/mL. DNA content of the treated cells increased; however, because of DNA degradation, its quality was decreased.Conclusion: In conclusion, graphene oxide at both nano- and micro-scale damages cell physiology and increases cell population in the S phase of the cell cycle.Keywords: apoptosis, cell cycle, fibroblast cell, graphene, TP53 geneHashemi EAkhavan OShamsara MAnsari Majd SSanati MHDaliri Joupari MFarmany ADove Medical Pressarticleapoptosiscell cyclefibroblast cellgraphenetp53 geneMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 6201-6209 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	apoptosis cell cycle fibroblast cell graphene tp53 gene Medicine (General) R5-920
spellingShingle	apoptosis cell cycle fibroblast cell graphene tp53 gene Medicine (General) R5-920 Hashemi E Akhavan O Shamsara M Ansari Majd S Sanati MH Daliri Joupari M Farmany A Graphene Oxide Negatively Regulates Cell Cycle in Embryonic Fibroblast Cells
description	Ehsan Hashemi,1– 3 Omid Akhavan,4 Mehdi Shamsara,1,2 Saeid Ansari Majd,2 Mohammad Hossein Sanati,5 Morteza Daliri Joupari,1,2 Abbas Farmany6 1Animal Biotechnology Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran; 2National Research Center for Transgenic Mouse, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran; 3Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran; 4Department of Physics, Sharif University of Technology, Tehran, Iran; 5Medical Genetics Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran; 6Dental Research Center, Hamadan University of Medical Sciences, Hamadan, IranCorrespondence: Morteza Daliri Joupari; Abbas Farmany Tel +98 21 4458-0340Fax +98 21 44580393Email daliri@nigeb.ac.ir; a.farmany@ut.ac.irBackground: Unique properties of graphene and its derivatives make them attractive in the field of nanomedicine. However, the mass application of graphene might lead to side effects, which has not been properly addressed in previous studies, especially with regard to its effect on the cell cycle.Methods: The effect of two concentrations (100 and 200 μg/mL) of nano- and microsized graphene oxide (nGO and mGO) on apoptosis, cell cycle, and ROS generation was studied. The effect of both sizes on viability and genotoxicity of the embryonic fibroblast cell cycle was evaluated. MTT and flow cytometry were applied to evaluate the effects of graphene oxide (GO) nanosheets on viability of cells. Apoptosis and cell cycle were analyzed by flow cytometry.Results: The results of this study showed that GO disturbed the cell cycle and nGO impaired cell viability by inducing cell apoptosis. Interestingly, both nGO and mGO blocked the cell cycle in the S phase, which is a critical phase of the cell cycle. Upregulation of TP53-gene transcripts was also detected in both nGO- and mGO-treated cells compared to the control, especially at 200 μg/mL. DNA content of the treated cells increased; however, because of DNA degradation, its quality was decreased.Conclusion: In conclusion, graphene oxide at both nano- and micro-scale damages cell physiology and increases cell population in the S phase of the cell cycle.Keywords: apoptosis, cell cycle, fibroblast cell, graphene, TP53 gene
format	article
author	Hashemi E Akhavan O Shamsara M Ansari Majd S Sanati MH Daliri Joupari M Farmany A
author_facet	Hashemi E Akhavan O Shamsara M Ansari Majd S Sanati MH Daliri Joupari M Farmany A
author_sort	Hashemi E
title	Graphene Oxide Negatively Regulates Cell Cycle in Embryonic Fibroblast Cells
title_short	Graphene Oxide Negatively Regulates Cell Cycle in Embryonic Fibroblast Cells
title_full	Graphene Oxide Negatively Regulates Cell Cycle in Embryonic Fibroblast Cells
title_fullStr	Graphene Oxide Negatively Regulates Cell Cycle in Embryonic Fibroblast Cells
title_full_unstemmed	Graphene Oxide Negatively Regulates Cell Cycle in Embryonic Fibroblast Cells
title_sort	graphene oxide negatively regulates cell cycle in embryonic fibroblast cells
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/c3faad23619a48518718450e5cae03d3
work_keys_str_mv	AT hashemie grapheneoxidenegativelyregulatescellcycleinembryonicfibroblastcells AT akhavano grapheneoxidenegativelyregulatescellcycleinembryonicfibroblastcells AT shamsaram grapheneoxidenegativelyregulatescellcycleinembryonicfibroblastcells AT ansarimajds grapheneoxidenegativelyregulatescellcycleinembryonicfibroblastcells AT sanatimh grapheneoxidenegativelyregulatescellcycleinembryonicfibroblastcells AT dalirijouparim grapheneoxidenegativelyregulatescellcycleinembryonicfibroblastcells AT farmanya grapheneoxidenegativelyregulatescellcycleinembryonicfibroblastcells
_version_	1718397142368780288

Graphene Oxide Negatively Regulates Cell Cycle in Embryonic Fibroblast Cells

Ejemplares similares