A chemical probe based on the PreQ1 metabolite enables transcriptome-wide mapping of binding sites
The small modified nucleotide PreQ1 binds to the PreQ1 riboswitch and regulates gene expression by inducing RNA conformation change. Here the authors design and characterize a specific preQ1-derived probe by x-ray crystallography, mass spectral analysis and transcriptome-wide using Chem-CLIP.
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Nature Portfolio
2021
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oai:doaj.org-article:c3fe0cb68393406ba791bbafef33d2f32021-12-02T18:01:42ZA chemical probe based on the PreQ1 metabolite enables transcriptome-wide mapping of binding sites10.1038/s41467-021-25973-x2041-1723https://doaj.org/article/c3fe0cb68393406ba791bbafef33d2f32021-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25973-xhttps://doaj.org/toc/2041-1723The small modified nucleotide PreQ1 binds to the PreQ1 riboswitch and regulates gene expression by inducing RNA conformation change. Here the authors design and characterize a specific preQ1-derived probe by x-ray crystallography, mass spectral analysis and transcriptome-wide using Chem-CLIP.Sumirtha BalaratnamCurran RhodesDesta Doro BumeColleen ConnellyChristopher C. LaiJames A. KelleyKamyar YazdaniPhilip J. HomanDanny IncarnatoTomoyuki NumataJohn S. Schneekloth JrNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-15 (2021) |
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DOAJ |
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DOAJ |
| language |
EN |
| topic |
Science Q |
| spellingShingle |
Science Q Sumirtha Balaratnam Curran Rhodes Desta Doro Bume Colleen Connelly Christopher C. Lai James A. Kelley Kamyar Yazdani Philip J. Homan Danny Incarnato Tomoyuki Numata John S. Schneekloth Jr A chemical probe based on the PreQ1 metabolite enables transcriptome-wide mapping of binding sites |
| description |
The small modified nucleotide PreQ1 binds to the PreQ1 riboswitch and regulates gene expression by inducing RNA conformation change. Here the authors design and characterize a specific preQ1-derived probe by x-ray crystallography, mass spectral analysis and transcriptome-wide using Chem-CLIP. |
| format |
article |
| author |
Sumirtha Balaratnam Curran Rhodes Desta Doro Bume Colleen Connelly Christopher C. Lai James A. Kelley Kamyar Yazdani Philip J. Homan Danny Incarnato Tomoyuki Numata John S. Schneekloth Jr |
| author_facet |
Sumirtha Balaratnam Curran Rhodes Desta Doro Bume Colleen Connelly Christopher C. Lai James A. Kelley Kamyar Yazdani Philip J. Homan Danny Incarnato Tomoyuki Numata John S. Schneekloth Jr |
| author_sort |
Sumirtha Balaratnam |
| title |
A chemical probe based on the PreQ1 metabolite enables transcriptome-wide mapping of binding sites |
| title_short |
A chemical probe based on the PreQ1 metabolite enables transcriptome-wide mapping of binding sites |
| title_full |
A chemical probe based on the PreQ1 metabolite enables transcriptome-wide mapping of binding sites |
| title_fullStr |
A chemical probe based on the PreQ1 metabolite enables transcriptome-wide mapping of binding sites |
| title_full_unstemmed |
A chemical probe based on the PreQ1 metabolite enables transcriptome-wide mapping of binding sites |
| title_sort |
chemical probe based on the preq1 metabolite enables transcriptome-wide mapping of binding sites |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/c3fe0cb68393406ba791bbafef33d2f3 |
| work_keys_str_mv |
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