A high-density SNP map for accurate mapping of seed fibre QTL in Brassica napus L.
A high density genetic linkage map for the complex allotetraploid crop species Brassica napus (oilseed rape) was constructed in a late-generation recombinant inbred line (RIL) population, using genome-wide single nucleotide polymorphism (SNP) markers assayed by the Brassica 60 K Infinium BeadChip Ar...
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oai:doaj.org-article:c4002daea0334c36b6147e9f111166052021-11-18T08:40:23ZA high-density SNP map for accurate mapping of seed fibre QTL in Brassica napus L.1932-620310.1371/journal.pone.0083052https://doaj.org/article/c4002daea0334c36b6147e9f111166052013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24386142/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203A high density genetic linkage map for the complex allotetraploid crop species Brassica napus (oilseed rape) was constructed in a late-generation recombinant inbred line (RIL) population, using genome-wide single nucleotide polymorphism (SNP) markers assayed by the Brassica 60 K Infinium BeadChip Array. The linkage map contains 9164 SNP markers covering 1832.9 cM. 1232 bins account for 7648 of the markers. A subset of 2795 SNP markers, with an average distance of 0.66 cM between adjacent markers, was applied for QTL mapping of seed colour and the cell wall fiber components acid detergent lignin (ADL), cellulose and hemicellulose. After phenotypic analyses across four different environments a total of 11 QTL were detected for seed colour and fiber traits. The high-density map considerably improved QTL resolution compared to the previous low-density maps. A previously identified major QTL with very high effects on seed colour and ADL was pinpointed to a narrow genome interval on chromosome A09, while a minor QTL explaining 8.1% to 14.1% of variation for ADL was detected on chromosome C05. Five and three QTL accounting for 4.7% to 21.9% and 7.3% to 16.9% of the phenotypic variation for cellulose and hemicellulose, respectively, were also detected. To our knowledge this is the first description of QTL for seed cellulose and hemicellulose in B. napus, representing interesting new targets for improving oil content. The high density SNP genetic map enables navigation from interesting B. napus QTL to Brassica genome sequences, giving useful new information for understanding the genetics of key seed quality traits in rapeseed.Liezhao LiuCunmin QuBenjamin WittkopBin YiYang XiaoYajun HeRod J SnowdonJiana LiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e83052 (2013) |
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Medicine R Science Q |
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Medicine R Science Q Liezhao Liu Cunmin Qu Benjamin Wittkop Bin Yi Yang Xiao Yajun He Rod J Snowdon Jiana Li A high-density SNP map for accurate mapping of seed fibre QTL in Brassica napus L. |
| description |
A high density genetic linkage map for the complex allotetraploid crop species Brassica napus (oilseed rape) was constructed in a late-generation recombinant inbred line (RIL) population, using genome-wide single nucleotide polymorphism (SNP) markers assayed by the Brassica 60 K Infinium BeadChip Array. The linkage map contains 9164 SNP markers covering 1832.9 cM. 1232 bins account for 7648 of the markers. A subset of 2795 SNP markers, with an average distance of 0.66 cM between adjacent markers, was applied for QTL mapping of seed colour and the cell wall fiber components acid detergent lignin (ADL), cellulose and hemicellulose. After phenotypic analyses across four different environments a total of 11 QTL were detected for seed colour and fiber traits. The high-density map considerably improved QTL resolution compared to the previous low-density maps. A previously identified major QTL with very high effects on seed colour and ADL was pinpointed to a narrow genome interval on chromosome A09, while a minor QTL explaining 8.1% to 14.1% of variation for ADL was detected on chromosome C05. Five and three QTL accounting for 4.7% to 21.9% and 7.3% to 16.9% of the phenotypic variation for cellulose and hemicellulose, respectively, were also detected. To our knowledge this is the first description of QTL for seed cellulose and hemicellulose in B. napus, representing interesting new targets for improving oil content. The high density SNP genetic map enables navigation from interesting B. napus QTL to Brassica genome sequences, giving useful new information for understanding the genetics of key seed quality traits in rapeseed. |
| format |
article |
| author |
Liezhao Liu Cunmin Qu Benjamin Wittkop Bin Yi Yang Xiao Yajun He Rod J Snowdon Jiana Li |
| author_facet |
Liezhao Liu Cunmin Qu Benjamin Wittkop Bin Yi Yang Xiao Yajun He Rod J Snowdon Jiana Li |
| author_sort |
Liezhao Liu |
| title |
A high-density SNP map for accurate mapping of seed fibre QTL in Brassica napus L. |
| title_short |
A high-density SNP map for accurate mapping of seed fibre QTL in Brassica napus L. |
| title_full |
A high-density SNP map for accurate mapping of seed fibre QTL in Brassica napus L. |
| title_fullStr |
A high-density SNP map for accurate mapping of seed fibre QTL in Brassica napus L. |
| title_full_unstemmed |
A high-density SNP map for accurate mapping of seed fibre QTL in Brassica napus L. |
| title_sort |
high-density snp map for accurate mapping of seed fibre qtl in brassica napus l. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/c4002daea0334c36b6147e9f11116605 |
| work_keys_str_mv |
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