Early OXA-48-Producing <italic toggle="yes">Enterobacterales</italic> Isolates Recovered in a Spanish Hospital Reveal a Complex Introduction Dominated by Sequence Type 11 (ST11) and ST405 <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Clones

ABSTRACT Carbapenemase-producing Enterobacterales (CPE) have become an important public health concern. In our hospital, VIM enzymes were first detected in 2005, Klebsiella pneumoniae carbapenemase (KPC) enzymes in 2009, and OXA-48 enzymes in 2012. We assess the population biology of the first OXA-4...

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Autores principales:	Desirèe Gijón, Ana P. Tedim, Aránzazu Valverde, Irene Rodríguez, María-Isabel Morosini, Teresa M. Coque, Marina Manrique, Eduardo Pareja, Raquel Tobes, Patricia Ruiz-Garbajosa, Rafael Cantón
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	OXA-48 carbapenemase VIM-1 CTX-M-15 Enterobacterales ST11 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/c41266cbfcfa49ae80ccde013e8dc6ee
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spelling	oai:doaj.org-article:c41266cbfcfa49ae80ccde013e8dc6ee2021-11-15T15:29:17ZEarly OXA-48-Producing <italic toggle="yes">Enterobacterales</italic> Isolates Recovered in a Spanish Hospital Reveal a Complex Introduction Dominated by Sequence Type 11 (ST11) and ST405 <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Clones10.1128/mSphere.00080-202379-5042https://doaj.org/article/c41266cbfcfa49ae80ccde013e8dc6ee2020-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00080-20https://doaj.org/toc/2379-5042ABSTRACT Carbapenemase-producing Enterobacterales (CPE) have become an important public health concern. In our hospital, VIM enzymes were first detected in 2005, Klebsiella pneumoniae carbapenemase (KPC) enzymes in 2009, and OXA-48 enzymes in 2012. We assess the population biology of the first OXA-48-producing Enterobacterales isolates recovered in our hospital (2012 to 2013) where infections by other carbapenemases had been endemic for several years. Over a 21-month period, 71 isolates (61 Klebsiella pneumoniae, 5 Escherichia coli, 2 Klebsiella aerogenes, and 1 each of Enterobacter cloacae, Klebsiella oxytoca, and Citrobacter amalonaticus) recovered from clinical and surveillance specimens from 57 patients (22.8% nonhospitalized) were investigated for OXA-48-like-producing enzymes. Analyses for characterization and determination of the location of the blaOXA-48 gene, plasmid transferability, sequence, and clonal relatedness were performed. Most of the isolates also coproduced CTX-M-15 (57/71, 80.3%) and/or VIM-1 (7/71, 9.8%). K. pneumoniae was predominantly identified as sequence type 11 (ST11) (63.4%) and ST405 (9.8%) and E. coli as ST540, ST1406, ST3163, and ST4301. The blaOXA-48 gene was part of Tn1999.2 located at the tir gene of plasmids (ca. ≥50 kb) of the IncL/M group, also carrying blaVIM-1 and blaCTX-M-15 genes. We selected one ST11 K. pneumoniae isolate for whole-genome sequencing in which we studied the plasmid containing the blaOXA-48 gene. This plasmid was compared with indexed plasmids existing in NCBI database by the use of BRIG and MAUVE. Our data suggest a rapid spread of blaOXA-48 genes between commonly isolated high-risk clones of Enterobacterales species, frequently associated with antibiotic resistance. Moreover, the emergence of the multiresistant ST11 K. pneumoniae clone among nonhospitalized patients emphasizes the difficulty of preventing its dissemination into the community. IMPORTANCE We present results of microbiological analysis of the first Enterobacterales isolates that were isolated in 2012 in our institution expressing OXA-48 carbapenemase. This enzyme confers resistance to carbapenems, an important group of antibiotics widely used in the hospitals. OXA-48 carbapenemase is currently present in many parts of the world, but it is found particularly frequently in the Mediterranean area. It was disseminated at the Ramón y Cajal Hospital and found to be associated with a particular Klebsiella pneumoniae strain, so-called high-risk clone ST11, which was previously found in our institution in association with other enzymes such as CTX-M-15, VIM-1, and KPC-3. This clone might have acquired a plasmid harboring the blaOXA-48 gene. Our results point out the importance of local epidemiology in the dissemination and maintenance of multidrug-resistant bacteria.Desirèe GijónAna P. TedimAránzazu ValverdeIrene RodríguezMaría-Isabel MorosiniTeresa M. CoqueMarina ManriqueEduardo ParejaRaquel TobesPatricia Ruiz-GarbajosaRafael CantónAmerican Society for MicrobiologyarticleOXA-48carbapenemaseVIM-1CTX-M-15EnterobacteralesST11MicrobiologyQR1-502ENmSphere, Vol 5, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	OXA-48 carbapenemase VIM-1 CTX-M-15 Enterobacterales ST11 Microbiology QR1-502
spellingShingle	OXA-48 carbapenemase VIM-1 CTX-M-15 Enterobacterales ST11 Microbiology QR1-502 Desirèe Gijón Ana P. Tedim Aránzazu Valverde Irene Rodríguez María-Isabel Morosini Teresa M. Coque Marina Manrique Eduardo Pareja Raquel Tobes Patricia Ruiz-Garbajosa Rafael Cantón Early OXA-48-Producing <italic toggle="yes">Enterobacterales</italic> Isolates Recovered in a Spanish Hospital Reveal a Complex Introduction Dominated by Sequence Type 11 (ST11) and ST405 <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Clones
description	ABSTRACT Carbapenemase-producing Enterobacterales (CPE) have become an important public health concern. In our hospital, VIM enzymes were first detected in 2005, Klebsiella pneumoniae carbapenemase (KPC) enzymes in 2009, and OXA-48 enzymes in 2012. We assess the population biology of the first OXA-48-producing Enterobacterales isolates recovered in our hospital (2012 to 2013) where infections by other carbapenemases had been endemic for several years. Over a 21-month period, 71 isolates (61 Klebsiella pneumoniae, 5 Escherichia coli, 2 Klebsiella aerogenes, and 1 each of Enterobacter cloacae, Klebsiella oxytoca, and Citrobacter amalonaticus) recovered from clinical and surveillance specimens from 57 patients (22.8% nonhospitalized) were investigated for OXA-48-like-producing enzymes. Analyses for characterization and determination of the location of the blaOXA-48 gene, plasmid transferability, sequence, and clonal relatedness were performed. Most of the isolates also coproduced CTX-M-15 (57/71, 80.3%) and/or VIM-1 (7/71, 9.8%). K. pneumoniae was predominantly identified as sequence type 11 (ST11) (63.4%) and ST405 (9.8%) and E. coli as ST540, ST1406, ST3163, and ST4301. The blaOXA-48 gene was part of Tn1999.2 located at the tir gene of plasmids (ca. ≥50 kb) of the IncL/M group, also carrying blaVIM-1 and blaCTX-M-15 genes. We selected one ST11 K. pneumoniae isolate for whole-genome sequencing in which we studied the plasmid containing the blaOXA-48 gene. This plasmid was compared with indexed plasmids existing in NCBI database by the use of BRIG and MAUVE. Our data suggest a rapid spread of blaOXA-48 genes between commonly isolated high-risk clones of Enterobacterales species, frequently associated with antibiotic resistance. Moreover, the emergence of the multiresistant ST11 K. pneumoniae clone among nonhospitalized patients emphasizes the difficulty of preventing its dissemination into the community. IMPORTANCE We present results of microbiological analysis of the first Enterobacterales isolates that were isolated in 2012 in our institution expressing OXA-48 carbapenemase. This enzyme confers resistance to carbapenems, an important group of antibiotics widely used in the hospitals. OXA-48 carbapenemase is currently present in many parts of the world, but it is found particularly frequently in the Mediterranean area. It was disseminated at the Ramón y Cajal Hospital and found to be associated with a particular Klebsiella pneumoniae strain, so-called high-risk clone ST11, which was previously found in our institution in association with other enzymes such as CTX-M-15, VIM-1, and KPC-3. This clone might have acquired a plasmid harboring the blaOXA-48 gene. Our results point out the importance of local epidemiology in the dissemination and maintenance of multidrug-resistant bacteria.
format	article
author	Desirèe Gijón Ana P. Tedim Aránzazu Valverde Irene Rodríguez María-Isabel Morosini Teresa M. Coque Marina Manrique Eduardo Pareja Raquel Tobes Patricia Ruiz-Garbajosa Rafael Cantón
author_facet	Desirèe Gijón Ana P. Tedim Aránzazu Valverde Irene Rodríguez María-Isabel Morosini Teresa M. Coque Marina Manrique Eduardo Pareja Raquel Tobes Patricia Ruiz-Garbajosa Rafael Cantón
author_sort	Desirèe Gijón
title	Early OXA-48-Producing <italic toggle="yes">Enterobacterales</italic> Isolates Recovered in a Spanish Hospital Reveal a Complex Introduction Dominated by Sequence Type 11 (ST11) and ST405 <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Clones
title_short	Early OXA-48-Producing <italic toggle="yes">Enterobacterales</italic> Isolates Recovered in a Spanish Hospital Reveal a Complex Introduction Dominated by Sequence Type 11 (ST11) and ST405 <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Clones
title_full	Early OXA-48-Producing <italic toggle="yes">Enterobacterales</italic> Isolates Recovered in a Spanish Hospital Reveal a Complex Introduction Dominated by Sequence Type 11 (ST11) and ST405 <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Clones
title_fullStr	Early OXA-48-Producing <italic toggle="yes">Enterobacterales</italic> Isolates Recovered in a Spanish Hospital Reveal a Complex Introduction Dominated by Sequence Type 11 (ST11) and ST405 <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Clones
title_full_unstemmed	Early OXA-48-Producing <italic toggle="yes">Enterobacterales</italic> Isolates Recovered in a Spanish Hospital Reveal a Complex Introduction Dominated by Sequence Type 11 (ST11) and ST405 <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Clones
title_sort	early oxa-48-producing <italic toggle="yes">enterobacterales</italic> isolates recovered in a spanish hospital reveal a complex introduction dominated by sequence type 11 (st11) and st405 <named-content content-type="genus-species">klebsiella pneumoniae</named-content> clones
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/c41266cbfcfa49ae80ccde013e8dc6ee
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Early OXA-48-Producing <italic toggle="yes">Enterobacterales</italic> Isolates Recovered in a Spanish Hospital Reveal a Complex Introduction Dominated by Sequence Type 11 (ST11) and ST405 <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Clones

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