A two-phase innate host response to alphavirus infection identified by mRNP-tagging in vivo.

A concept fundamental to viral pathogenesis is that infection induces specific changes within the host cell, within specific tissues, or within the entire animal. These changes are reflected in a cascade of altered transcription patterns evident during infection. However, elucidation of this cascade...

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Autores principales:	Jennifer L Konopka, Luiz O Penalva, Joseph M Thompson, Laura J White, Clayton W Beard, Jack D Keene, Robert E Johnston
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2007
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c4134ec5a1674c579635d18a29172451
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spelling	oai:doaj.org-article:c4134ec5a1674c579635d18a291724512021-11-25T05:46:47ZA two-phase innate host response to alphavirus infection identified by mRNP-tagging in vivo.1553-73661553-737410.1371/journal.ppat.0030199https://doaj.org/article/c4134ec5a1674c579635d18a291724512007-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18215114/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374A concept fundamental to viral pathogenesis is that infection induces specific changes within the host cell, within specific tissues, or within the entire animal. These changes are reflected in a cascade of altered transcription patterns evident during infection. However, elucidation of this cascade in vivo has been limited by a general inability to distinguish changes occurring in the minority of infected cells from those in surrounding uninfected cells. To circumvent this inherent limitation of traditional gene expression profiling methods, an innovative mRNP-tagging technique was implemented to isolate host mRNA specifically from infected cells in vitro as well as in vivo following Venezuelan equine encephalitis virus (VEE) infection. This technique facilitated a direct characterization of the host defense response specifically within the first cells infected with VEE, while simultaneous total RNA analysis assessed the collective response of both the infected and uninfected cells. The result was a unique, multifaceted profile of the early response to VEE infection in primary dendritic cells, as well as in the draining lymph node, the initially targeted tissue in the mouse model. A dynamic environment of complex interactions was revealed, and suggested a two-step innate response in which activation of a subset of host genes in infected cells subsequently leads to activation of the surrounding uninfected cells. Our findings suggest that the application of viral mRNP-tagging systems, as introduced here, will facilitate a much more detailed understanding of the highly coordinated host response to infectious agents.Jennifer L KonopkaLuiz O PenalvaJoseph M ThompsonLaura J WhiteClayton W BeardJack D KeeneRobert E JohnstonPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 3, Iss 12, p e199 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Jennifer L Konopka Luiz O Penalva Joseph M Thompson Laura J White Clayton W Beard Jack D Keene Robert E Johnston A two-phase innate host response to alphavirus infection identified by mRNP-tagging in vivo.
description	A concept fundamental to viral pathogenesis is that infection induces specific changes within the host cell, within specific tissues, or within the entire animal. These changes are reflected in a cascade of altered transcription patterns evident during infection. However, elucidation of this cascade in vivo has been limited by a general inability to distinguish changes occurring in the minority of infected cells from those in surrounding uninfected cells. To circumvent this inherent limitation of traditional gene expression profiling methods, an innovative mRNP-tagging technique was implemented to isolate host mRNA specifically from infected cells in vitro as well as in vivo following Venezuelan equine encephalitis virus (VEE) infection. This technique facilitated a direct characterization of the host defense response specifically within the first cells infected with VEE, while simultaneous total RNA analysis assessed the collective response of both the infected and uninfected cells. The result was a unique, multifaceted profile of the early response to VEE infection in primary dendritic cells, as well as in the draining lymph node, the initially targeted tissue in the mouse model. A dynamic environment of complex interactions was revealed, and suggested a two-step innate response in which activation of a subset of host genes in infected cells subsequently leads to activation of the surrounding uninfected cells. Our findings suggest that the application of viral mRNP-tagging systems, as introduced here, will facilitate a much more detailed understanding of the highly coordinated host response to infectious agents.
format	article
author	Jennifer L Konopka Luiz O Penalva Joseph M Thompson Laura J White Clayton W Beard Jack D Keene Robert E Johnston
author_facet	Jennifer L Konopka Luiz O Penalva Joseph M Thompson Laura J White Clayton W Beard Jack D Keene Robert E Johnston
author_sort	Jennifer L Konopka
title	A two-phase innate host response to alphavirus infection identified by mRNP-tagging in vivo.
title_short	A two-phase innate host response to alphavirus infection identified by mRNP-tagging in vivo.
title_full	A two-phase innate host response to alphavirus infection identified by mRNP-tagging in vivo.
title_fullStr	A two-phase innate host response to alphavirus infection identified by mRNP-tagging in vivo.
title_full_unstemmed	A two-phase innate host response to alphavirus infection identified by mRNP-tagging in vivo.
title_sort	two-phase innate host response to alphavirus infection identified by mrnp-tagging in vivo.
publisher	Public Library of Science (PLoS)
publishDate	2007
url	https://doaj.org/article/c4134ec5a1674c579635d18a29172451
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A two-phase innate host response to alphavirus infection identified by mRNP-tagging in vivo.

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