Plasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis

Abstract The anaphase promoting complex/cyclosome (APC/C) is a highly conserved multi-subunit E3 ubiquitin ligase that controls mitotic division in eukaryotic cells by tagging cell cycle regulators for proteolysis. APC3 is a key component that contributes to APC/C function. Plasmodium, the causative...

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Autores principales:	Richard J. Wall, David J. P. Ferguson, Aline Freville, Blandine Franke-Fayard, Declan Brady, Mohammad Zeeshan, Andrew R. Bottrill, Sally Wheatley, Andrew M. Fry, Chris J. Janse, Hiroyuki Yamano, Anthony A. Holder, David S. Guttery, Rita Tewari
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c4146aca53b342b192bb9f54c10c319c
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spelling	oai:doaj.org-article:c4146aca53b342b192bb9f54c10c319c2021-12-02T15:09:06ZPlasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis10.1038/s41598-018-23871-92045-2322https://doaj.org/article/c4146aca53b342b192bb9f54c10c319c2018-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-23871-9https://doaj.org/toc/2045-2322Abstract The anaphase promoting complex/cyclosome (APC/C) is a highly conserved multi-subunit E3 ubiquitin ligase that controls mitotic division in eukaryotic cells by tagging cell cycle regulators for proteolysis. APC3 is a key component that contributes to APC/C function. Plasmodium, the causative agent of malaria, undergoes atypical mitotic division during its life cycle. Only a small subset of APC/C components has been identified in Plasmodium and their involvement in atypical cell division is not well understood. Here, using reverse genetics we examined the localisation and function of APC3 in Plasmodium berghei. APC3 was observed as a single focus that co-localised with the centriolar plaque during asexual cell division in schizonts, whereas it appeared as multiple foci in male gametocytes. Functional studies using gene disruption and conditional knockdown revealed essential roles of APC3 during these mitotic stages with loss resulting in a lack of chromosome condensation, abnormal cytokinesis and absence of microgamete formation. Overall, our data suggest that Plasmodium utilises unique cell cycle machinery to coordinate various processes during endomitosis, and this warrants further investigation in future studies.Richard J. WallDavid J. P. FergusonAline FrevilleBlandine Franke-FayardDeclan BradyMohammad ZeeshanAndrew R. BottrillSally WheatleyAndrew M. FryChris J. JanseHiroyuki YamanoAnthony A. HolderDavid S. GutteryRita TewariNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Richard J. Wall David J. P. Ferguson Aline Freville Blandine Franke-Fayard Declan Brady Mohammad Zeeshan Andrew R. Bottrill Sally Wheatley Andrew M. Fry Chris J. Janse Hiroyuki Yamano Anthony A. Holder David S. Guttery Rita Tewari Plasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis
description	Abstract The anaphase promoting complex/cyclosome (APC/C) is a highly conserved multi-subunit E3 ubiquitin ligase that controls mitotic division in eukaryotic cells by tagging cell cycle regulators for proteolysis. APC3 is a key component that contributes to APC/C function. Plasmodium, the causative agent of malaria, undergoes atypical mitotic division during its life cycle. Only a small subset of APC/C components has been identified in Plasmodium and their involvement in atypical cell division is not well understood. Here, using reverse genetics we examined the localisation and function of APC3 in Plasmodium berghei. APC3 was observed as a single focus that co-localised with the centriolar plaque during asexual cell division in schizonts, whereas it appeared as multiple foci in male gametocytes. Functional studies using gene disruption and conditional knockdown revealed essential roles of APC3 during these mitotic stages with loss resulting in a lack of chromosome condensation, abnormal cytokinesis and absence of microgamete formation. Overall, our data suggest that Plasmodium utilises unique cell cycle machinery to coordinate various processes during endomitosis, and this warrants further investigation in future studies.
format	article
author	Richard J. Wall David J. P. Ferguson Aline Freville Blandine Franke-Fayard Declan Brady Mohammad Zeeshan Andrew R. Bottrill Sally Wheatley Andrew M. Fry Chris J. Janse Hiroyuki Yamano Anthony A. Holder David S. Guttery Rita Tewari
author_facet	Richard J. Wall David J. P. Ferguson Aline Freville Blandine Franke-Fayard Declan Brady Mohammad Zeeshan Andrew R. Bottrill Sally Wheatley Andrew M. Fry Chris J. Janse Hiroyuki Yamano Anthony A. Holder David S. Guttery Rita Tewari
author_sort	Richard J. Wall
title	Plasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis
title_short	Plasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis
title_full	Plasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis
title_fullStr	Plasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis
title_full_unstemmed	Plasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis
title_sort	plasmodium apc3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/c4146aca53b342b192bb9f54c10c319c
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Plasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis

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