Functional characterization of the short neuropeptide F receptor in the desert locust, Schistocerca gregaria.

Whereas short neuropeptide F (sNPF) has already been reported to stimulate feeding behaviour in a variety of insect species, the opposite effect was observed in the desert locust. In the present study, we cloned a G protein-coupled receptor (GPCR) cDNA from the desert locust, Schistocerca gregaria....

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Autores principales:	Senne Dillen, Sven Zels, Heleen Verlinden, Jornt Spit, Pieter Van Wielendaele, Jozef Vanden Broeck
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c416396977ba4161aa5a06fcb28ea9b3
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spelling	oai:doaj.org-article:c416396977ba4161aa5a06fcb28ea9b32021-11-18T08:02:36ZFunctional characterization of the short neuropeptide F receptor in the desert locust, Schistocerca gregaria.1932-620310.1371/journal.pone.0053604https://doaj.org/article/c416396977ba4161aa5a06fcb28ea9b32013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23308260/?tool=EBIhttps://doaj.org/toc/1932-6203Whereas short neuropeptide F (sNPF) has already been reported to stimulate feeding behaviour in a variety of insect species, the opposite effect was observed in the desert locust. In the present study, we cloned a G protein-coupled receptor (GPCR) cDNA from the desert locust, Schistocerca gregaria. Cell-based functional analysis of this receptor indicated that it is activated by both known isoforms of Schgr-sNPF in a concentration dependent manner, with EC(50) values in the nanomolar range. This Schgr-sNPF receptor constitutes the first functionally characterized peptide GPCR in locusts. The in vivo effects of the sNPF signalling pathway on the regulation of feeding in locusts were further studied by knocking down the newly identified Schgr-sNPF receptor by means of RNA interference, as well as by means of peptide injection studies. While injection of sNPF caused an inhibitory effect on food uptake in the desert locust, knocking down the corresponding peptide receptor resulted in an increase of total food uptake when compared to control animals. This is the first comprehensive study in which a clearly negative correlation is described between the sNPF signalling pathway and feeding, prompting a reconsideration of the diverse roles of sNPFs in the physiology of insects.Senne DillenSven ZelsHeleen VerlindenJornt SpitPieter Van WielendaeleJozef Vanden BroeckPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e53604 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Senne Dillen Sven Zels Heleen Verlinden Jornt Spit Pieter Van Wielendaele Jozef Vanden Broeck Functional characterization of the short neuropeptide F receptor in the desert locust, Schistocerca gregaria.
description	Whereas short neuropeptide F (sNPF) has already been reported to stimulate feeding behaviour in a variety of insect species, the opposite effect was observed in the desert locust. In the present study, we cloned a G protein-coupled receptor (GPCR) cDNA from the desert locust, Schistocerca gregaria. Cell-based functional analysis of this receptor indicated that it is activated by both known isoforms of Schgr-sNPF in a concentration dependent manner, with EC(50) values in the nanomolar range. This Schgr-sNPF receptor constitutes the first functionally characterized peptide GPCR in locusts. The in vivo effects of the sNPF signalling pathway on the regulation of feeding in locusts were further studied by knocking down the newly identified Schgr-sNPF receptor by means of RNA interference, as well as by means of peptide injection studies. While injection of sNPF caused an inhibitory effect on food uptake in the desert locust, knocking down the corresponding peptide receptor resulted in an increase of total food uptake when compared to control animals. This is the first comprehensive study in which a clearly negative correlation is described between the sNPF signalling pathway and feeding, prompting a reconsideration of the diverse roles of sNPFs in the physiology of insects.
format	article
author	Senne Dillen Sven Zels Heleen Verlinden Jornt Spit Pieter Van Wielendaele Jozef Vanden Broeck
author_facet	Senne Dillen Sven Zels Heleen Verlinden Jornt Spit Pieter Van Wielendaele Jozef Vanden Broeck
author_sort	Senne Dillen
title	Functional characterization of the short neuropeptide F receptor in the desert locust, Schistocerca gregaria.
title_short	Functional characterization of the short neuropeptide F receptor in the desert locust, Schistocerca gregaria.
title_full	Functional characterization of the short neuropeptide F receptor in the desert locust, Schistocerca gregaria.
title_fullStr	Functional characterization of the short neuropeptide F receptor in the desert locust, Schistocerca gregaria.
title_full_unstemmed	Functional characterization of the short neuropeptide F receptor in the desert locust, Schistocerca gregaria.
title_sort	functional characterization of the short neuropeptide f receptor in the desert locust, schistocerca gregaria.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/c416396977ba4161aa5a06fcb28ea9b3
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Functional characterization of the short neuropeptide F receptor in the desert locust, Schistocerca gregaria.

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