The blood oxygen level dependent (BOLD) effect of in-vitro myoglobin and hemoglobin
Abstract The presence of deoxygenated hemoglobin (Hb) results in a drop in T2 and T2* in magnetic resonance imaging (MRI), known as the blood oxygenation level-dependent (BOLD-)effect. The purpose of this study was to investigate if deoxygenated myoglobin (Mb) exerts a BOLD-like effect. Equine Met-M...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/c4198135e1e34c48ac17c3d6ce382633 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:c4198135e1e34c48ac17c3d6ce382633 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:c4198135e1e34c48ac17c3d6ce3826332021-12-02T18:24:54ZThe blood oxygen level dependent (BOLD) effect of in-vitro myoglobin and hemoglobin10.1038/s41598-021-90908-x2045-2322https://doaj.org/article/c4198135e1e34c48ac17c3d6ce3826332021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90908-xhttps://doaj.org/toc/2045-2322Abstract The presence of deoxygenated hemoglobin (Hb) results in a drop in T2 and T2* in magnetic resonance imaging (MRI), known as the blood oxygenation level-dependent (BOLD-)effect. The purpose of this study was to investigate if deoxygenated myoglobin (Mb) exerts a BOLD-like effect. Equine Met-Mb powder was dissolved and converted to oxygenated Mb. T1, T2, T2*-maps and BOLD-bSSFP images at 3Tesla were used to scan 22 Mb samples and 12 Hb samples at room air, deoxygenation, reoxygenation and after chemical reduction. In Mb, T2 and T2* mapping showed a significant decrease after deoxygenation (− 25% and − 12%, p < 0.01), increase after subsequent reoxygenation (+ 17% and 0% vs. room air, p < 0.01), and finally a decrease in T2 after chemical reduction (− 28%, p < 0.01). An opposite trend was observed with T1 for each stage, while chemical reduction reduced BOLD-bSSFP signal (− 3%, p < 0.01). Similar deflections were seen at oxygenation changes in Hb. The T1 changes suggests that the oxygen content has been changed in the specimen. The shortening of transverse relaxation times in T2 and T2*-mapping after deoxygenation in Mb specimens are highly indicative of a BOLD-like effect.Dominik P. GuenschMatthias C. MichelStefan P. HuettenmoserBernd JungPatrik GulacAdrian SegiserSarah L. LongnusKady FischerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Dominik P. Guensch Matthias C. Michel Stefan P. Huettenmoser Bernd Jung Patrik Gulac Adrian Segiser Sarah L. Longnus Kady Fischer The blood oxygen level dependent (BOLD) effect of in-vitro myoglobin and hemoglobin |
| description |
Abstract The presence of deoxygenated hemoglobin (Hb) results in a drop in T2 and T2* in magnetic resonance imaging (MRI), known as the blood oxygenation level-dependent (BOLD-)effect. The purpose of this study was to investigate if deoxygenated myoglobin (Mb) exerts a BOLD-like effect. Equine Met-Mb powder was dissolved and converted to oxygenated Mb. T1, T2, T2*-maps and BOLD-bSSFP images at 3Tesla were used to scan 22 Mb samples and 12 Hb samples at room air, deoxygenation, reoxygenation and after chemical reduction. In Mb, T2 and T2* mapping showed a significant decrease after deoxygenation (− 25% and − 12%, p < 0.01), increase after subsequent reoxygenation (+ 17% and 0% vs. room air, p < 0.01), and finally a decrease in T2 after chemical reduction (− 28%, p < 0.01). An opposite trend was observed with T1 for each stage, while chemical reduction reduced BOLD-bSSFP signal (− 3%, p < 0.01). Similar deflections were seen at oxygenation changes in Hb. The T1 changes suggests that the oxygen content has been changed in the specimen. The shortening of transverse relaxation times in T2 and T2*-mapping after deoxygenation in Mb specimens are highly indicative of a BOLD-like effect. |
| format |
article |
| author |
Dominik P. Guensch Matthias C. Michel Stefan P. Huettenmoser Bernd Jung Patrik Gulac Adrian Segiser Sarah L. Longnus Kady Fischer |
| author_facet |
Dominik P. Guensch Matthias C. Michel Stefan P. Huettenmoser Bernd Jung Patrik Gulac Adrian Segiser Sarah L. Longnus Kady Fischer |
| author_sort |
Dominik P. Guensch |
| title |
The blood oxygen level dependent (BOLD) effect of in-vitro myoglobin and hemoglobin |
| title_short |
The blood oxygen level dependent (BOLD) effect of in-vitro myoglobin and hemoglobin |
| title_full |
The blood oxygen level dependent (BOLD) effect of in-vitro myoglobin and hemoglobin |
| title_fullStr |
The blood oxygen level dependent (BOLD) effect of in-vitro myoglobin and hemoglobin |
| title_full_unstemmed |
The blood oxygen level dependent (BOLD) effect of in-vitro myoglobin and hemoglobin |
| title_sort |
blood oxygen level dependent (bold) effect of in-vitro myoglobin and hemoglobin |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/c4198135e1e34c48ac17c3d6ce382633 |
| work_keys_str_mv |
AT dominikpguensch thebloodoxygenleveldependentboldeffectofinvitromyoglobinandhemoglobin AT matthiascmichel thebloodoxygenleveldependentboldeffectofinvitromyoglobinandhemoglobin AT stefanphuettenmoser thebloodoxygenleveldependentboldeffectofinvitromyoglobinandhemoglobin AT berndjung thebloodoxygenleveldependentboldeffectofinvitromyoglobinandhemoglobin AT patrikgulac thebloodoxygenleveldependentboldeffectofinvitromyoglobinandhemoglobin AT adriansegiser thebloodoxygenleveldependentboldeffectofinvitromyoglobinandhemoglobin AT sarahllongnus thebloodoxygenleveldependentboldeffectofinvitromyoglobinandhemoglobin AT kadyfischer thebloodoxygenleveldependentboldeffectofinvitromyoglobinandhemoglobin AT dominikpguensch bloodoxygenleveldependentboldeffectofinvitromyoglobinandhemoglobin AT matthiascmichel bloodoxygenleveldependentboldeffectofinvitromyoglobinandhemoglobin AT stefanphuettenmoser bloodoxygenleveldependentboldeffectofinvitromyoglobinandhemoglobin AT berndjung bloodoxygenleveldependentboldeffectofinvitromyoglobinandhemoglobin AT patrikgulac bloodoxygenleveldependentboldeffectofinvitromyoglobinandhemoglobin AT adriansegiser bloodoxygenleveldependentboldeffectofinvitromyoglobinandhemoglobin AT sarahllongnus bloodoxygenleveldependentboldeffectofinvitromyoglobinandhemoglobin AT kadyfischer bloodoxygenleveldependentboldeffectofinvitromyoglobinandhemoglobin |
| _version_ |
1718378122466820096 |