The discovery of a novel knockdown resistance (kdr) mutation A1007G on Aedes aegypti (Diptera: Culicidae) from Malaysia

Abstract The usage of insecticide rendered the successful vector control program with the high usage of the pyrethroid. However, the intensive and extensive use of pyrethroid, causing resistance in Aedes aegypti and hampered the control program. Knockdown resistance (kdr) resulting from the Voltage-...

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Autores principales:	Wan Fatma Zuharah, Maryam Sufian
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c41b6791d27a4380a949023843b9edfa
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spelling	oai:doaj.org-article:c41b6791d27a4380a949023843b9edfa2021-12-02T13:33:51ZThe discovery of a novel knockdown resistance (kdr) mutation A1007G on Aedes aegypti (Diptera: Culicidae) from Malaysia10.1038/s41598-021-84669-w2045-2322https://doaj.org/article/c41b6791d27a4380a949023843b9edfa2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84669-whttps://doaj.org/toc/2045-2322Abstract The usage of insecticide rendered the successful vector control program with the high usage of the pyrethroid. However, the intensive and extensive use of pyrethroid, causing resistance in Aedes aegypti and hampered the control program. Knockdown resistance (kdr) resulting from the Voltage-Gated Sodium Channel (VGSC) is one of the mechanisms of resistance in pyrethroid group insecticide. Investigating the phenotypic status of Ae. aegypti mosquitoes is a lead in knowing the current resistance status and as an indicator of the genotypic resistance. In this study, we investigate the resistance in phenotypic and genotypic of Ae. aegypti with a new kdr mutation point A1007G was detected. Using the adult bioassay, we tested the phenotypic resistance from the Selangor state against 0.75% permethrin, 0.05% deltamethrin with and without the addition of PBO synergist. Permethrin-resistant and deltamethrin-resistant, including susceptible samples, were subjected to genotyping analysis on mutation point in domain II and domain III of Voltage-Gated Sodium Channel (VGSC). Adult bioassay revealed that the Ae. aegypti was highly resistance toward 0.75% permethrin and 0.05% deltamethrin. The bioassay with the presence of PBO synergist showed an increment of mortality rate, but Ae. aegypti status is still resistance towards both insecticides. Genotyping result showed that three common kdr mutations (S989P, V1016G, and F1534C) have existed in the Ae. aegypti population. A new novel mutation on A1007G was also detected in this population, which is the first time reported. This study has brought a piece of information on the current resistance status in Ae. aegypti in Malaysia. The detection of new mutation point of A1007G has added the knowledge on the resistance in mosquitoes. Thus, this study will aid with the decision making in the usage of insecticides in the vector control program; before this invaluable insecticide rendered ineffective in killing mosquitoes.Wan Fatma ZuharahMaryam SufianNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wan Fatma Zuharah Maryam Sufian The discovery of a novel knockdown resistance (kdr) mutation A1007G on Aedes aegypti (Diptera: Culicidae) from Malaysia
description	Abstract The usage of insecticide rendered the successful vector control program with the high usage of the pyrethroid. However, the intensive and extensive use of pyrethroid, causing resistance in Aedes aegypti and hampered the control program. Knockdown resistance (kdr) resulting from the Voltage-Gated Sodium Channel (VGSC) is one of the mechanisms of resistance in pyrethroid group insecticide. Investigating the phenotypic status of Ae. aegypti mosquitoes is a lead in knowing the current resistance status and as an indicator of the genotypic resistance. In this study, we investigate the resistance in phenotypic and genotypic of Ae. aegypti with a new kdr mutation point A1007G was detected. Using the adult bioassay, we tested the phenotypic resistance from the Selangor state against 0.75% permethrin, 0.05% deltamethrin with and without the addition of PBO synergist. Permethrin-resistant and deltamethrin-resistant, including susceptible samples, were subjected to genotyping analysis on mutation point in domain II and domain III of Voltage-Gated Sodium Channel (VGSC). Adult bioassay revealed that the Ae. aegypti was highly resistance toward 0.75% permethrin and 0.05% deltamethrin. The bioassay with the presence of PBO synergist showed an increment of mortality rate, but Ae. aegypti status is still resistance towards both insecticides. Genotyping result showed that three common kdr mutations (S989P, V1016G, and F1534C) have existed in the Ae. aegypti population. A new novel mutation on A1007G was also detected in this population, which is the first time reported. This study has brought a piece of information on the current resistance status in Ae. aegypti in Malaysia. The detection of new mutation point of A1007G has added the knowledge on the resistance in mosquitoes. Thus, this study will aid with the decision making in the usage of insecticides in the vector control program; before this invaluable insecticide rendered ineffective in killing mosquitoes.
format	article
author	Wan Fatma Zuharah Maryam Sufian
author_facet	Wan Fatma Zuharah Maryam Sufian
author_sort	Wan Fatma Zuharah
title	The discovery of a novel knockdown resistance (kdr) mutation A1007G on Aedes aegypti (Diptera: Culicidae) from Malaysia
title_short	The discovery of a novel knockdown resistance (kdr) mutation A1007G on Aedes aegypti (Diptera: Culicidae) from Malaysia
title_full	The discovery of a novel knockdown resistance (kdr) mutation A1007G on Aedes aegypti (Diptera: Culicidae) from Malaysia
title_fullStr	The discovery of a novel knockdown resistance (kdr) mutation A1007G on Aedes aegypti (Diptera: Culicidae) from Malaysia
title_full_unstemmed	The discovery of a novel knockdown resistance (kdr) mutation A1007G on Aedes aegypti (Diptera: Culicidae) from Malaysia
title_sort	discovery of a novel knockdown resistance (kdr) mutation a1007g on aedes aegypti (diptera: culicidae) from malaysia
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c41b6791d27a4380a949023843b9edfa
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The discovery of a novel knockdown resistance (kdr) mutation A1007G on Aedes aegypti (Diptera: Culicidae) from Malaysia

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