Transmitted HIV drug resistance and subtype patterns among blood donors in Poland

Abstract Surveillance on the HIV molecular variability, risk of drug resistance transmission and evolution of novel viral variants among blood donors remains an understudied aspect of hemovigilance. This nationwide study analyses patterns of HIV diversity and transmitted resistance mutations. Study...

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Autores principales:	Miłosz Parczewski, Ewa Sulkowska, Anna Urbańska, Kaja Scheibe, Karol Serwin, Piotr Grabarczyk
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c41bc06ff2154bff959322f0c286f71f
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spelling	oai:doaj.org-article:c41bc06ff2154bff959322f0c286f71f2021-12-02T16:04:26ZTransmitted HIV drug resistance and subtype patterns among blood donors in Poland10.1038/s41598-021-92210-22045-2322https://doaj.org/article/c41bc06ff2154bff959322f0c286f71f2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92210-2https://doaj.org/toc/2045-2322Abstract Surveillance on the HIV molecular variability, risk of drug resistance transmission and evolution of novel viral variants among blood donors remains an understudied aspect of hemovigilance. This nationwide study analyses patterns of HIV diversity and transmitted resistance mutations. Study included 185 samples from the first time and repeat blood donors with HIV infection identified by molecular assay. HIV protease, reverse transcriptase and integrase were sequenced using population methods. Drug resistance mutation (DRM) patterns were analyzed based on the Stanford Interpretation Algorithm and standardized lists of transmitted mutations. Phylogeny was used to investigate subtyping, clustering and recombination patterns. HIV-1 subtype B (89.2%) followed by subtype A6 (7.6%) were predominant, while in three (1.6%) cases, novel recombinant B/A6 variants were identified. Non-B variants were more common among repeat donors (14.5%) compared to the first time ones (1.8%), p = 0.011, with higher frequency (9.9%) of A6 variant in the repeat donor group, p = 0.04. Major NRTI DRMs were observed in 3.8%, NNRTI and PI in 0.6% and INSTI 1.1% of cases. Additionally, E157Q polymorphism was observed in 9.8% and L74I in 11.5% of integrase sequences. Transmission of drug resistance among blood donors remains infrequent. Subtype patters increase in complexity with emergence of novel intersubtype A6B recombinants.Miłosz ParczewskiEwa SulkowskaAnna UrbańskaKaja ScheibeKarol SerwinPiotr GrabarczykNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Miłosz Parczewski Ewa Sulkowska Anna Urbańska Kaja Scheibe Karol Serwin Piotr Grabarczyk Transmitted HIV drug resistance and subtype patterns among blood donors in Poland
description	Abstract Surveillance on the HIV molecular variability, risk of drug resistance transmission and evolution of novel viral variants among blood donors remains an understudied aspect of hemovigilance. This nationwide study analyses patterns of HIV diversity and transmitted resistance mutations. Study included 185 samples from the first time and repeat blood donors with HIV infection identified by molecular assay. HIV protease, reverse transcriptase and integrase were sequenced using population methods. Drug resistance mutation (DRM) patterns were analyzed based on the Stanford Interpretation Algorithm and standardized lists of transmitted mutations. Phylogeny was used to investigate subtyping, clustering and recombination patterns. HIV-1 subtype B (89.2%) followed by subtype A6 (7.6%) were predominant, while in three (1.6%) cases, novel recombinant B/A6 variants were identified. Non-B variants were more common among repeat donors (14.5%) compared to the first time ones (1.8%), p = 0.011, with higher frequency (9.9%) of A6 variant in the repeat donor group, p = 0.04. Major NRTI DRMs were observed in 3.8%, NNRTI and PI in 0.6% and INSTI 1.1% of cases. Additionally, E157Q polymorphism was observed in 9.8% and L74I in 11.5% of integrase sequences. Transmission of drug resistance among blood donors remains infrequent. Subtype patters increase in complexity with emergence of novel intersubtype A6B recombinants.
format	article
author	Miłosz Parczewski Ewa Sulkowska Anna Urbańska Kaja Scheibe Karol Serwin Piotr Grabarczyk
author_facet	Miłosz Parczewski Ewa Sulkowska Anna Urbańska Kaja Scheibe Karol Serwin Piotr Grabarczyk
author_sort	Miłosz Parczewski
title	Transmitted HIV drug resistance and subtype patterns among blood donors in Poland
title_short	Transmitted HIV drug resistance and subtype patterns among blood donors in Poland
title_full	Transmitted HIV drug resistance and subtype patterns among blood donors in Poland
title_fullStr	Transmitted HIV drug resistance and subtype patterns among blood donors in Poland
title_full_unstemmed	Transmitted HIV drug resistance and subtype patterns among blood donors in Poland
title_sort	transmitted hiv drug resistance and subtype patterns among blood donors in poland
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c41bc06ff2154bff959322f0c286f71f
work_keys_str_mv	AT miłoszparczewski transmittedhivdrugresistanceandsubtypepatternsamongblooddonorsinpoland AT ewasulkowska transmittedhivdrugresistanceandsubtypepatternsamongblooddonorsinpoland AT annaurbanska transmittedhivdrugresistanceandsubtypepatternsamongblooddonorsinpoland AT kajascheibe transmittedhivdrugresistanceandsubtypepatternsamongblooddonorsinpoland AT karolserwin transmittedhivdrugresistanceandsubtypepatternsamongblooddonorsinpoland AT piotrgrabarczyk transmittedhivdrugresistanceandsubtypepatternsamongblooddonorsinpoland
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Transmitted HIV drug resistance and subtype patterns among blood donors in Poland

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