Role of Enteric Glia as Bridging Element between Gut Inflammation and Visceral Pain Consolidation during Acute Colitis in Rats

Acute inflammation is particularly relevant in the pathogenesis of visceral hypersensitivity associated with inflammatory bowel diseases. Glia within the enteric nervous system, as well as within the central nervous system, contributes to neuroplasticity during inflammation, but whether enteric glia...

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Autores principales:	Elena Lucarini, Luisa Seguella, Martina Vincenzi, Carmen Parisio, Laura Micheli, Alessandra Toti, Chiara Corpetti, Alessandro Del Re, Silvia Squillace, Daniela Maftei, Roberta Lattanzi, Carla Ghelardini, Lorenzo Di Cesare Mannelli, Giuseppe Esposito
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	inflammatory bowel disease S100β TRPV1 receptors enteric glia astrocytes myenteric plexus Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c41ee61c78ce4f869de220d6a4220f7c
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spelling	oai:doaj.org-article:c41ee61c78ce4f869de220d6a4220f7c2021-11-25T16:50:36ZRole of Enteric Glia as Bridging Element between Gut Inflammation and Visceral Pain Consolidation during Acute Colitis in Rats10.3390/biomedicines91116712227-9059https://doaj.org/article/c41ee61c78ce4f869de220d6a4220f7c2021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1671https://doaj.org/toc/2227-9059Acute inflammation is particularly relevant in the pathogenesis of visceral hypersensitivity associated with inflammatory bowel diseases. Glia within the enteric nervous system, as well as within the central nervous system, contributes to neuroplasticity during inflammation, but whether enteric glia has the potential to modify visceral sensitivity following colitis is still unknown. This work aimed to investigate the occurrence of changes in the neuron–glial networks controlling visceral perception along the gut–brain axis during colitis, and to assess the effects of peripheral glial manipulation. Enteric glia activity was altered by the poison fluorocitrate (FC; 10 µmol kg<sup>−1</sup> i.p.) before inducing colitis in animals (2,4-dinitrobenzenesulfonic acid, DNBS; 30 mg in 0.25 mL EtOH 50%), and visceral sensitivity, colon damage, and glia activation along the pain pathway were studied. FC injection significantly reduced the visceral hyperalgesia, the histological damage, and the immune activation caused by DNBS. Intestinal inflammation is associated with a parallel overexpression of TRPV1 and S100β along the gut–brain axis (colonic myenteric plexuses, dorsal root ganglion, and periaqueductal grey area). This effect was prevented by FC. Peripheral glia activity modulation emerges as a promising strategy for counteracting visceral pain induced by colitis.Elena LucariniLuisa SeguellaMartina VincenziCarmen ParisioLaura MicheliAlessandra TotiChiara CorpettiAlessandro Del ReSilvia SquillaceDaniela MafteiRoberta LattanziCarla GhelardiniLorenzo Di Cesare MannelliGiuseppe EspositoMDPI AGarticleinflammatory bowel diseaseS100βTRPV1 receptorsenteric gliaastrocytesmyenteric plexusBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1671, p 1671 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	inflammatory bowel disease S100β TRPV1 receptors enteric glia astrocytes myenteric plexus Biology (General) QH301-705.5
spellingShingle	inflammatory bowel disease S100β TRPV1 receptors enteric glia astrocytes myenteric plexus Biology (General) QH301-705.5 Elena Lucarini Luisa Seguella Martina Vincenzi Carmen Parisio Laura Micheli Alessandra Toti Chiara Corpetti Alessandro Del Re Silvia Squillace Daniela Maftei Roberta Lattanzi Carla Ghelardini Lorenzo Di Cesare Mannelli Giuseppe Esposito Role of Enteric Glia as Bridging Element between Gut Inflammation and Visceral Pain Consolidation during Acute Colitis in Rats
description	Acute inflammation is particularly relevant in the pathogenesis of visceral hypersensitivity associated with inflammatory bowel diseases. Glia within the enteric nervous system, as well as within the central nervous system, contributes to neuroplasticity during inflammation, but whether enteric glia has the potential to modify visceral sensitivity following colitis is still unknown. This work aimed to investigate the occurrence of changes in the neuron–glial networks controlling visceral perception along the gut–brain axis during colitis, and to assess the effects of peripheral glial manipulation. Enteric glia activity was altered by the poison fluorocitrate (FC; 10 µmol kg<sup>−1</sup> i.p.) before inducing colitis in animals (2,4-dinitrobenzenesulfonic acid, DNBS; 30 mg in 0.25 mL EtOH 50%), and visceral sensitivity, colon damage, and glia activation along the pain pathway were studied. FC injection significantly reduced the visceral hyperalgesia, the histological damage, and the immune activation caused by DNBS. Intestinal inflammation is associated with a parallel overexpression of TRPV1 and S100β along the gut–brain axis (colonic myenteric plexuses, dorsal root ganglion, and periaqueductal grey area). This effect was prevented by FC. Peripheral glia activity modulation emerges as a promising strategy for counteracting visceral pain induced by colitis.
format	article
author	Elena Lucarini Luisa Seguella Martina Vincenzi Carmen Parisio Laura Micheli Alessandra Toti Chiara Corpetti Alessandro Del Re Silvia Squillace Daniela Maftei Roberta Lattanzi Carla Ghelardini Lorenzo Di Cesare Mannelli Giuseppe Esposito
author_facet	Elena Lucarini Luisa Seguella Martina Vincenzi Carmen Parisio Laura Micheli Alessandra Toti Chiara Corpetti Alessandro Del Re Silvia Squillace Daniela Maftei Roberta Lattanzi Carla Ghelardini Lorenzo Di Cesare Mannelli Giuseppe Esposito
author_sort	Elena Lucarini
title	Role of Enteric Glia as Bridging Element between Gut Inflammation and Visceral Pain Consolidation during Acute Colitis in Rats
title_short	Role of Enteric Glia as Bridging Element between Gut Inflammation and Visceral Pain Consolidation during Acute Colitis in Rats
title_full	Role of Enteric Glia as Bridging Element between Gut Inflammation and Visceral Pain Consolidation during Acute Colitis in Rats
title_fullStr	Role of Enteric Glia as Bridging Element between Gut Inflammation and Visceral Pain Consolidation during Acute Colitis in Rats
title_full_unstemmed	Role of Enteric Glia as Bridging Element between Gut Inflammation and Visceral Pain Consolidation during Acute Colitis in Rats
title_sort	role of enteric glia as bridging element between gut inflammation and visceral pain consolidation during acute colitis in rats
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c41ee61c78ce4f869de220d6a4220f7c
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Role of Enteric Glia as Bridging Element between Gut Inflammation and Visceral Pain Consolidation during Acute Colitis in Rats

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