Efficacy of retinol palmitate eye drops for dry eye in rabbits with lacrimal gland resection

Akito Odaka,1 Hiroshi Toshida,2 Toshihiko Ohta,2 Nobuhito Tabuchi,3,4 Daisuke Koike,4 Chikako Suto,3 Akira Murakami31Human and Environmental Safety Evaluation Center, Lion Corporation, Kanagawa, Japan; 2Department of Ophthalmology, Juntendo University Shizuoka Hospital, Shizuoka, Japan; 3Department...

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Autores principales:	Odaka A, Toshida H, Ohta T, Tabuchi N, Koike D, Suto C, Murakami A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/c425b75d23c94569b1bcc1bbbc82bbdb
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spelling	oai:doaj.org-article:c425b75d23c94569b1bcc1bbbc82bbdb2021-12-02T02:24:33ZEfficacy of retinol palmitate eye drops for dry eye in rabbits with lacrimal gland resection1177-54671177-5483https://doaj.org/article/c425b75d23c94569b1bcc1bbbc82bbdb2012-10-01T00:00:00Zhttp://www.dovepress.com/efficacy-of-retinol-palmitate-eye-drops-for-dry-eye-in-rabbits-with-la-a11150https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Akito Odaka,1 Hiroshi Toshida,2 Toshihiko Ohta,2 Nobuhito Tabuchi,3,4 Daisuke Koike,4 Chikako Suto,3 Akira Murakami31Human and Environmental Safety Evaluation Center, Lion Corporation, Kanagawa, Japan; 2Department of Ophthalmology, Juntendo University Shizuoka Hospital, Shizuoka, Japan; 3Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan; 4Pharmaceutical Research Laboratories No 1, Research and Development Headquarters, Lion Corporation, Kanagawa, JapanPurpose: We examined the efficacy of retinol palmitate (VApal) for dry eyes using dry eye model rabbits whose lacrimal glands were resected.Materials and methods: After alkaline injury on keratoconjunctival epithelium, VApal eye drops were administered 6 times a day for 7 days. The efficacy of VApal was also compared with that of 0.1% hyaluronic acid eye drops.Results: The fluorescein staining and rose bengal scores showed a significant decrease compared with the score in the vehicle group at 7 days (P &lt; 0.05) in the 1000 IU/mL VApal group and at both 3 days (P &lt; 0.05) and 7 days (P &lt; 0.01) in the 1500 IU/mL VApal group. Histological examination revealed recovery of the corneal epithelium, and PAS staining disclosed the recovery of mucin-producing lower palpebral conjunctival goblet cells after 7 days in the 1500 IU/mL VApal group compared with the vehicle group. Results from impression cytology showed a significant increase in density of conjunctival goblet cells compared with that in the vehicle group after 7 days in the 1000 IU/mL VApal group and after 3 and 7 days in the 1500 IU/mL VApal group. There were no significant changes in tear flow in either group. Topical application of VApal at 1500 IU/mL showed greater improvement than 0.1% hyaluronic acid in both fluorescein and rose bengal score and in the density of conjunctival goblet cells.Conclusion: It is suggested that VApal is effective for the improvement of keratoconjunctival epithelial damage associated with tear abnormalities, such as dry eyes.Keywords: cornea, dry eye, hyaluronic acid, vitamin A, wound healing, retinol palmitateOdaka AToshida HOhta TTabuchi NKoike DSuto CMurakami ADove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2012, Iss default, Pp 1585-1593 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Odaka A Toshida H Ohta T Tabuchi N Koike D Suto C Murakami A Efficacy of retinol palmitate eye drops for dry eye in rabbits with lacrimal gland resection
description	Akito Odaka,1 Hiroshi Toshida,2 Toshihiko Ohta,2 Nobuhito Tabuchi,3,4 Daisuke Koike,4 Chikako Suto,3 Akira Murakami31Human and Environmental Safety Evaluation Center, Lion Corporation, Kanagawa, Japan; 2Department of Ophthalmology, Juntendo University Shizuoka Hospital, Shizuoka, Japan; 3Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan; 4Pharmaceutical Research Laboratories No 1, Research and Development Headquarters, Lion Corporation, Kanagawa, JapanPurpose: We examined the efficacy of retinol palmitate (VApal) for dry eyes using dry eye model rabbits whose lacrimal glands were resected.Materials and methods: After alkaline injury on keratoconjunctival epithelium, VApal eye drops were administered 6 times a day for 7 days. The efficacy of VApal was also compared with that of 0.1% hyaluronic acid eye drops.Results: The fluorescein staining and rose bengal scores showed a significant decrease compared with the score in the vehicle group at 7 days (P &lt; 0.05) in the 1000 IU/mL VApal group and at both 3 days (P &lt; 0.05) and 7 days (P &lt; 0.01) in the 1500 IU/mL VApal group. Histological examination revealed recovery of the corneal epithelium, and PAS staining disclosed the recovery of mucin-producing lower palpebral conjunctival goblet cells after 7 days in the 1500 IU/mL VApal group compared with the vehicle group. Results from impression cytology showed a significant increase in density of conjunctival goblet cells compared with that in the vehicle group after 7 days in the 1000 IU/mL VApal group and after 3 and 7 days in the 1500 IU/mL VApal group. There were no significant changes in tear flow in either group. Topical application of VApal at 1500 IU/mL showed greater improvement than 0.1% hyaluronic acid in both fluorescein and rose bengal score and in the density of conjunctival goblet cells.Conclusion: It is suggested that VApal is effective for the improvement of keratoconjunctival epithelial damage associated with tear abnormalities, such as dry eyes.Keywords: cornea, dry eye, hyaluronic acid, vitamin A, wound healing, retinol palmitate
format	article
author	Odaka A Toshida H Ohta T Tabuchi N Koike D Suto C Murakami A
author_facet	Odaka A Toshida H Ohta T Tabuchi N Koike D Suto C Murakami A
author_sort	Odaka A
title	Efficacy of retinol palmitate eye drops for dry eye in rabbits with lacrimal gland resection
title_short	Efficacy of retinol palmitate eye drops for dry eye in rabbits with lacrimal gland resection
title_full	Efficacy of retinol palmitate eye drops for dry eye in rabbits with lacrimal gland resection
title_fullStr	Efficacy of retinol palmitate eye drops for dry eye in rabbits with lacrimal gland resection
title_full_unstemmed	Efficacy of retinol palmitate eye drops for dry eye in rabbits with lacrimal gland resection
title_sort	efficacy of retinol palmitate eye drops for dry eye in rabbits with lacrimal gland resection
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/c425b75d23c94569b1bcc1bbbc82bbdb
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Efficacy of retinol palmitate eye drops for dry eye in rabbits with lacrimal gland resection

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