Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress
Abstract ACE2 and TMPRSS2 are key players on SARS-CoV-2 entry into host cells. However, it is still unclear whether expression levels of these factors could reflect disease severity. Here, a case–control study was conducted with 213 SARS-CoV-2 positive individuals where cases were defined as COVID-1...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/c427b492385a4d0f84ccc25eea32d672 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:c427b492385a4d0f84ccc25eea32d672 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:c427b492385a4d0f84ccc25eea32d6722021-12-02T16:49:13ZAssociation between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress10.1038/s41598-021-88944-82045-2322https://doaj.org/article/c427b492385a4d0f84ccc25eea32d6722021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88944-8https://doaj.org/toc/2045-2322Abstract ACE2 and TMPRSS2 are key players on SARS-CoV-2 entry into host cells. However, it is still unclear whether expression levels of these factors could reflect disease severity. Here, a case–control study was conducted with 213 SARS-CoV-2 positive individuals where cases were defined as COVID-19 patients with respiratory distress requiring oxygen support (N = 38) and controls were those with mild to moderate symptoms of the disease who did not need oxygen therapy along the entire clinical course (N = 175). ACE2 and TMPRSS2 mRNA levels were evaluated in nasopharyngeal swab samples by RT-qPCR and logistic regression analyzes were applied to estimate associations with respiratory outcomes. ACE2 and TMPRSS2 levels positively correlated with age, which was also strongly associated with respiratory distress. Increased nasopharyngeal ACE2 levels showed a protective effect against this outcome (adjOR = 0.30; 95% CI 0.09–0.91), while TMPRSS2/ACE2 ratio was associated with risk (adjOR = 4.28; 95% CI 1.36–13.48). On stepwise regression, TMPRSS2/ACE2 ratio outperformed ACE2 to model COVID-19 severity. When nasopharyngeal swabs were compared to bronchoalveolar lavages in an independent cohort of COVID-19 patients under mechanical ventilation, similar expression levels of these genes were observed. These data suggest nasopharyngeal TMPRSS2/ACE2 as a promising candidate for further prediction models on COVID-19.Átila Duque RossiJoão Locke Ferreira de AraújoTailah Bernardo de AlmeidaMarcelo Ribeiro-AlvesCamila de Almeida VelozoJéssica Maciel de AlmeidaIsabela de Carvalho LeitãoSâmila Natiane FerreiraJéssica da Silva OliveiraHugo José AlvesHelena Toledo ScheidDébora Souza FaffeRafael Mello GalliezRenata Eliane de ÁvilaGustavo Gomes ResendeMauro Martins TeixeiraCOVID-19 UFRJ WorkgroupOrlando da Costa Ferreira JúniorTerezinha Marta P. P. CastiñeirasRenan Pedra SouzaAmilcar TanuriRenato Santana de AguiarShana Priscila Coutinho BarrosoCynthia Chester CardosoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Átila Duque Rossi João Locke Ferreira de Araújo Tailah Bernardo de Almeida Marcelo Ribeiro-Alves Camila de Almeida Velozo Jéssica Maciel de Almeida Isabela de Carvalho Leitão Sâmila Natiane Ferreira Jéssica da Silva Oliveira Hugo José Alves Helena Toledo Scheid Débora Souza Faffe Rafael Mello Galliez Renata Eliane de Ávila Gustavo Gomes Resende Mauro Martins Teixeira COVID-19 UFRJ Workgroup Orlando da Costa Ferreira Júnior Terezinha Marta P. P. Castiñeiras Renan Pedra Souza Amilcar Tanuri Renato Santana de Aguiar Shana Priscila Coutinho Barroso Cynthia Chester Cardoso Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress |
| description |
Abstract ACE2 and TMPRSS2 are key players on SARS-CoV-2 entry into host cells. However, it is still unclear whether expression levels of these factors could reflect disease severity. Here, a case–control study was conducted with 213 SARS-CoV-2 positive individuals where cases were defined as COVID-19 patients with respiratory distress requiring oxygen support (N = 38) and controls were those with mild to moderate symptoms of the disease who did not need oxygen therapy along the entire clinical course (N = 175). ACE2 and TMPRSS2 mRNA levels were evaluated in nasopharyngeal swab samples by RT-qPCR and logistic regression analyzes were applied to estimate associations with respiratory outcomes. ACE2 and TMPRSS2 levels positively correlated with age, which was also strongly associated with respiratory distress. Increased nasopharyngeal ACE2 levels showed a protective effect against this outcome (adjOR = 0.30; 95% CI 0.09–0.91), while TMPRSS2/ACE2 ratio was associated with risk (adjOR = 4.28; 95% CI 1.36–13.48). On stepwise regression, TMPRSS2/ACE2 ratio outperformed ACE2 to model COVID-19 severity. When nasopharyngeal swabs were compared to bronchoalveolar lavages in an independent cohort of COVID-19 patients under mechanical ventilation, similar expression levels of these genes were observed. These data suggest nasopharyngeal TMPRSS2/ACE2 as a promising candidate for further prediction models on COVID-19. |
| format |
article |
| author |
Átila Duque Rossi João Locke Ferreira de Araújo Tailah Bernardo de Almeida Marcelo Ribeiro-Alves Camila de Almeida Velozo Jéssica Maciel de Almeida Isabela de Carvalho Leitão Sâmila Natiane Ferreira Jéssica da Silva Oliveira Hugo José Alves Helena Toledo Scheid Débora Souza Faffe Rafael Mello Galliez Renata Eliane de Ávila Gustavo Gomes Resende Mauro Martins Teixeira COVID-19 UFRJ Workgroup Orlando da Costa Ferreira Júnior Terezinha Marta P. P. Castiñeiras Renan Pedra Souza Amilcar Tanuri Renato Santana de Aguiar Shana Priscila Coutinho Barroso Cynthia Chester Cardoso |
| author_facet |
Átila Duque Rossi João Locke Ferreira de Araújo Tailah Bernardo de Almeida Marcelo Ribeiro-Alves Camila de Almeida Velozo Jéssica Maciel de Almeida Isabela de Carvalho Leitão Sâmila Natiane Ferreira Jéssica da Silva Oliveira Hugo José Alves Helena Toledo Scheid Débora Souza Faffe Rafael Mello Galliez Renata Eliane de Ávila Gustavo Gomes Resende Mauro Martins Teixeira COVID-19 UFRJ Workgroup Orlando da Costa Ferreira Júnior Terezinha Marta P. P. Castiñeiras Renan Pedra Souza Amilcar Tanuri Renato Santana de Aguiar Shana Priscila Coutinho Barroso Cynthia Chester Cardoso |
| author_sort |
Átila Duque Rossi |
| title |
Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress |
| title_short |
Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress |
| title_full |
Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress |
| title_fullStr |
Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress |
| title_full_unstemmed |
Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress |
| title_sort |
association between ace2 and tmprss2 nasopharyngeal expression and covid-19 respiratory distress |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/c427b492385a4d0f84ccc25eea32d672 |
| work_keys_str_mv |
AT atiladuquerossi associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT joaolockeferreiradearaujo associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT tailahbernardodealmeida associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT marceloribeiroalves associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT camiladealmeidavelozo associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT jessicamacieldealmeida associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT isabeladecarvalholeitao associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT samilanatianeferreira associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT jessicadasilvaoliveira associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT hugojosealves associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT helenatoledoscheid associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT deborasouzafaffe associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT rafaelmellogalliez associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT renataelianedeavila associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT gustavogomesresende associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT mauromartinsteixeira associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT covid19ufrjworkgroup associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT orlandodacostaferreirajunior associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT terezinhamartappcastineiras associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT renanpedrasouza associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT amilcartanuri associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT renatosantanadeaguiar associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT shanapriscilacoutinhobarroso associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress AT cynthiachestercardoso associationbetweenace2andtmprss2nasopharyngealexpressionandcovid19respiratorydistress |
| _version_ |
1718383419654668288 |