Fabrication of a protein microarray by fluorous-fluorous interactions

Abstract Fluorous-modified surfaces have emerged as a powerful tool for the immobilization of fluorous-tagged biomolecules based on their specificity and the strength of fluorous-fluorous interactions. To fabricate a fluorous-based protein microarray, we designed two strategies for site-specific mod...

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Autores principales:	Ben-Yuan Li, Duane S. Juang, Avijit K. Adak, Kuo-Chu Hwang, Chun-Cheng Lin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c4282a7c9f2b4abe84c7a55fcf9501c2
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spelling	oai:doaj.org-article:c4282a7c9f2b4abe84c7a55fcf9501c22021-12-02T11:52:23ZFabrication of a protein microarray by fluorous-fluorous interactions10.1038/s41598-017-07571-42045-2322https://doaj.org/article/c4282a7c9f2b4abe84c7a55fcf9501c22017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07571-4https://doaj.org/toc/2045-2322Abstract Fluorous-modified surfaces have emerged as a powerful tool for the immobilization of fluorous-tagged biomolecules based on their specificity and the strength of fluorous-fluorous interactions. To fabricate a fluorous-based protein microarray, we designed two strategies for site-specific modification of proteins with a fluorous tag: attaching the fluorous tag to the C-termini of expressed proteins by native chemical ligation (NCL) or to the Fc domain of antibodies through boronic acid (BA)-diol interactions. The perfluoro-tagged proteins could be easily purified by fluorous-functionalized magnetic nanoparticles (MNPs) and immobilized on a fluorous chip with minimal non-specific adsorption. Importantly, proteins immobilized on the solid support through non-covalent fluorous-fluorous interactions were sufficiently stable to withstand continuous washing. We believe that this fluorous-fluorous immobilization strategy will be a highly valuable tool in protein microarray fabrication.Ben-Yuan LiDuane S. JuangAvijit K. AdakKuo-Chu HwangChun-Cheng LinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ben-Yuan Li Duane S. Juang Avijit K. Adak Kuo-Chu Hwang Chun-Cheng Lin Fabrication of a protein microarray by fluorous-fluorous interactions
description	Abstract Fluorous-modified surfaces have emerged as a powerful tool for the immobilization of fluorous-tagged biomolecules based on their specificity and the strength of fluorous-fluorous interactions. To fabricate a fluorous-based protein microarray, we designed two strategies for site-specific modification of proteins with a fluorous tag: attaching the fluorous tag to the C-termini of expressed proteins by native chemical ligation (NCL) or to the Fc domain of antibodies through boronic acid (BA)-diol interactions. The perfluoro-tagged proteins could be easily purified by fluorous-functionalized magnetic nanoparticles (MNPs) and immobilized on a fluorous chip with minimal non-specific adsorption. Importantly, proteins immobilized on the solid support through non-covalent fluorous-fluorous interactions were sufficiently stable to withstand continuous washing. We believe that this fluorous-fluorous immobilization strategy will be a highly valuable tool in protein microarray fabrication.
format	article
author	Ben-Yuan Li Duane S. Juang Avijit K. Adak Kuo-Chu Hwang Chun-Cheng Lin
author_facet	Ben-Yuan Li Duane S. Juang Avijit K. Adak Kuo-Chu Hwang Chun-Cheng Lin
author_sort	Ben-Yuan Li
title	Fabrication of a protein microarray by fluorous-fluorous interactions
title_short	Fabrication of a protein microarray by fluorous-fluorous interactions
title_full	Fabrication of a protein microarray by fluorous-fluorous interactions
title_fullStr	Fabrication of a protein microarray by fluorous-fluorous interactions
title_full_unstemmed	Fabrication of a protein microarray by fluorous-fluorous interactions
title_sort	fabrication of a protein microarray by fluorous-fluorous interactions
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c4282a7c9f2b4abe84c7a55fcf9501c2
work_keys_str_mv	AT benyuanli fabricationofaproteinmicroarraybyfluorousfluorousinteractions AT duanesjuang fabricationofaproteinmicroarraybyfluorousfluorousinteractions AT avijitkadak fabricationofaproteinmicroarraybyfluorousfluorousinteractions AT kuochuhwang fabricationofaproteinmicroarraybyfluorousfluorousinteractions AT chunchenglin fabricationofaproteinmicroarraybyfluorousfluorousinteractions
_version_	1718395068498313216

Fabrication of a protein microarray by fluorous-fluorous interactions

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