A Potent and Safer Anticancer and Antibacterial Taxus-Based Green Synthesized Silver Nanoparticle

Sona Sarli,1 Mohamad Reza Kalani,2,* Abdolvahab Moradi3,* 1Department of Chemistry, Arak Islamic Azad University, Arak, Iran; 2Medical Cellular and Molecular Research Center, School of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran; 3Department of Microbiology,...

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Autores principales:	Sarli S, Kalani MR, Moradi A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	taxus tree extract green synthesis silver nanoparticle antibacterial anticancer Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c428d3cd43e14e1e969831088d942c2f
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spelling	oai:doaj.org-article:c428d3cd43e14e1e969831088d942c2f2021-12-02T08:09:12ZA Potent and Safer Anticancer and Antibacterial Taxus-Based Green Synthesized Silver Nanoparticle1178-2013https://doaj.org/article/c428d3cd43e14e1e969831088d942c2f2020-05-01T00:00:00Zhttps://www.dovepress.com/a-potent-and-safer-anticancer-and-antibacterial-taxus-based-green-synt-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Sona Sarli,1 Mohamad Reza Kalani,2,* Abdolvahab Moradi3,* 1Department of Chemistry, Arak Islamic Azad University, Arak, Iran; 2Medical Cellular and Molecular Research Center, School of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran; 3Department of Microbiology, College of Medicine, Golestan University of Medical Sciences, Gorgan, Iran*These authors contributed equally to this workCorrespondence: Abdolvahab Moradi; Mohamad Reza Kalani Email abmoradi@gmail.com; kalanimr@yahoo.comPurpose: Paclitaxel is a generic drug produced based on Taxol which is an extract of Taxus tree, well known for its anticancer and antibacterial effects. This study was aimed at building up an agent with the antibacterial and anticancer benefits of both the silver ions and Taxol, together with less cytotoxic effects.Materials and Methods: Colloidal silver nanoparticles (AgNPs) were synthesized by reducing aqueous AgNO3 with aqueous Taxus leaf extract at nonphotomediated conditions, without any catalyst, template or surfactant. The AgNP production was confirmed by ultraviolet-visible (UV-VIS) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier-transform infrared (FTI) spectroscopy. The MTT assay for human breast cancer cells as well as the DAPI fluorescent staining microscopy tested the biocompatibility and anticancer effects of AgNPs, silver nitrate, and Taxol. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) techniques were performed to determine the shape and size of the nanoparticles. MTT assay showed the best inhibitory concentration of AgNPs on cancer cells. The antibacterial activity of the three case study materials was tested for gram-positive (Staphylococcus aureus) and gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) using well diffusion test.Results: This work proposes more anticancer effects for AgNP made by Taxus brevifolia extract, comparing Taxol solution. IC50 was observed as 3.1 mM for Taxol while 1.5 mM for new AgNP. Moreover, Taxus showed no antibacterial effects while the new AgNP showed a dose-dependent biocompatibility along with slightly more antibacterial effects (MIC: 1.6 and 6.6mM for gram-positive and -negative bacteria, respectively) comparing with silver nitrate solution (MIC: 1.5 and 6.2 mM for gram-positive and -negative bacteria, respectively).Conclusion: The production of herbal-mediated silver nanoparticles may be an efficient substitution for the silver nitrate–based medicines with less side effects.Keywords: Taxus tree extract, green synthesis, silver nanoparticle, antibacterial, anticancerSarli SKalani MRMoradi ADove Medical Pressarticletaxus tree extractgreen synthesissilver nanoparticleantibacterialanticancerMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 3791-3801 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	taxus tree extract green synthesis silver nanoparticle antibacterial anticancer Medicine (General) R5-920
spellingShingle	taxus tree extract green synthesis silver nanoparticle antibacterial anticancer Medicine (General) R5-920 Sarli S Kalani MR Moradi A A Potent and Safer Anticancer and Antibacterial Taxus-Based Green Synthesized Silver Nanoparticle
description	Sona Sarli,1 Mohamad Reza Kalani,2,* Abdolvahab Moradi3,* 1Department of Chemistry, Arak Islamic Azad University, Arak, Iran; 2Medical Cellular and Molecular Research Center, School of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran; 3Department of Microbiology, College of Medicine, Golestan University of Medical Sciences, Gorgan, Iran*These authors contributed equally to this workCorrespondence: Abdolvahab Moradi; Mohamad Reza Kalani Email abmoradi@gmail.com; kalanimr@yahoo.comPurpose: Paclitaxel is a generic drug produced based on Taxol which is an extract of Taxus tree, well known for its anticancer and antibacterial effects. This study was aimed at building up an agent with the antibacterial and anticancer benefits of both the silver ions and Taxol, together with less cytotoxic effects.Materials and Methods: Colloidal silver nanoparticles (AgNPs) were synthesized by reducing aqueous AgNO3 with aqueous Taxus leaf extract at nonphotomediated conditions, without any catalyst, template or surfactant. The AgNP production was confirmed by ultraviolet-visible (UV-VIS) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier-transform infrared (FTI) spectroscopy. The MTT assay for human breast cancer cells as well as the DAPI fluorescent staining microscopy tested the biocompatibility and anticancer effects of AgNPs, silver nitrate, and Taxol. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) techniques were performed to determine the shape and size of the nanoparticles. MTT assay showed the best inhibitory concentration of AgNPs on cancer cells. The antibacterial activity of the three case study materials was tested for gram-positive (Staphylococcus aureus) and gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) using well diffusion test.Results: This work proposes more anticancer effects for AgNP made by Taxus brevifolia extract, comparing Taxol solution. IC50 was observed as 3.1 mM for Taxol while 1.5 mM for new AgNP. Moreover, Taxus showed no antibacterial effects while the new AgNP showed a dose-dependent biocompatibility along with slightly more antibacterial effects (MIC: 1.6 and 6.6mM for gram-positive and -negative bacteria, respectively) comparing with silver nitrate solution (MIC: 1.5 and 6.2 mM for gram-positive and -negative bacteria, respectively).Conclusion: The production of herbal-mediated silver nanoparticles may be an efficient substitution for the silver nitrate–based medicines with less side effects.Keywords: Taxus tree extract, green synthesis, silver nanoparticle, antibacterial, anticancer
format	article
author	Sarli S Kalani MR Moradi A
author_facet	Sarli S Kalani MR Moradi A
author_sort	Sarli S
title	A Potent and Safer Anticancer and Antibacterial Taxus-Based Green Synthesized Silver Nanoparticle
title_short	A Potent and Safer Anticancer and Antibacterial Taxus-Based Green Synthesized Silver Nanoparticle
title_full	A Potent and Safer Anticancer and Antibacterial Taxus-Based Green Synthesized Silver Nanoparticle
title_fullStr	A Potent and Safer Anticancer and Antibacterial Taxus-Based Green Synthesized Silver Nanoparticle
title_full_unstemmed	A Potent and Safer Anticancer and Antibacterial Taxus-Based Green Synthesized Silver Nanoparticle
title_sort	potent and safer anticancer and antibacterial taxus-based green synthesized silver nanoparticle
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/c428d3cd43e14e1e969831088d942c2f
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A Potent and Safer Anticancer and Antibacterial Taxus-Based Green Synthesized Silver Nanoparticle

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