Possible Dissemination of Escherichia coli Sequence Type 410 Closely Related to B4/H24RxC in Ghana

Extra-intestinal pathogenic Escherichia coli (ExPEC) is one of the world’s leading causes of bloodstream infections with high mortality. Sequence type 410 (ST410) is an emerging ExPEC clone resistant to a wide range of antibiotics. In this study, we investigated the epidemiology of 21 ST410 E. coli...

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Autores principales:	Samiratu Mahazu, Isaac Prah, Alafate Ayibieke, Wakana Sato, Takaya Hayashi, Toshihiko Suzuki, Shiroh Iwanaga, Anthony Ablordey, Ryoichi Saito
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Extra-intestinal pathogenic E. coli (ExPEC) ST410 B3/H24Rx B4H24RxC Oxacillinase-181 (OXA-181) Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/c42ca45a6467457a960cc236579368b2
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spelling	oai:doaj.org-article:c42ca45a6467457a960cc236579368b22021-12-02T00:09:20ZPossible Dissemination of Escherichia coli Sequence Type 410 Closely Related to B4/H24RxC in Ghana1664-302X10.3389/fmicb.2021.770130https://doaj.org/article/c42ca45a6467457a960cc236579368b22021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.770130/fullhttps://doaj.org/toc/1664-302XExtra-intestinal pathogenic Escherichia coli (ExPEC) is one of the world’s leading causes of bloodstream infections with high mortality. Sequence type 410 (ST410) is an emerging ExPEC clone resistant to a wide range of antibiotics. In this study, we investigated the epidemiology of 21 ST410 E. coli isolates from two Ghanaian hospitals. We also investigated the isolates within a global context to provide further insight into the dissemination of this highly pathogenic clone. A phylogenetic tree of the 21 isolate genomes, along with 102 others from global collection, was constructed representing the ensuing clades and sub-clades of the ST: A/H53, B2/H24R, B3/H24Rx, and B4/H24RxC. The carbapenem-resistant sub-clade B4/H24RxC is reported to have emerged in the early 2000s when ST410 acquired an IncX3 plasmid carrying a blaOXA–181 carbapenemase gene, and a second carbapenemase gene, blaNDM–5, on a conserved IncFII plasmid in 2014. We identified, in this study, one blaOXA–181–carrying isolate belonging to B4/H24RxC sub-lineage and one carrying blaNDM–1 belonging to sub-lineage B3/H24Rx. The blaOXA–181 gene was found on a 51kb IncX3 plasmid; pEc1079_3. The majority (12/21) of our Ghanaian isolates were clustered with international strains described by previous authors as closely related strains to B4/H24RxC. Six others were clustered among the ESBL-associated sub-lineage B3/H24Rx and three with the globally disseminated sub-lineage B4/H24RxC. The results show that this highly pathogenic clone is disseminated in Ghana and, given its ability to transmit between hosts, it poses a serious threat and should be monitored closely.Samiratu MahazuSamiratu MahazuIsaac PrahIsaac PrahAlafate AyibiekeWakana SatoTakaya HayashiToshihiko SuzukiShiroh IwanagaAnthony AblordeyRyoichi SaitoFrontiers Media S.A.articleExtra-intestinal pathogenic E. coli (ExPEC)ST410B3/H24RxB4H24RxCOxacillinase-181 (OXA-181)MicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Extra-intestinal pathogenic E. coli (ExPEC) ST410 B3/H24Rx B4H24RxC Oxacillinase-181 (OXA-181) Microbiology QR1-502
spellingShingle	Extra-intestinal pathogenic E. coli (ExPEC) ST410 B3/H24Rx B4H24RxC Oxacillinase-181 (OXA-181) Microbiology QR1-502 Samiratu Mahazu Samiratu Mahazu Isaac Prah Isaac Prah Alafate Ayibieke Wakana Sato Takaya Hayashi Toshihiko Suzuki Shiroh Iwanaga Anthony Ablordey Ryoichi Saito Possible Dissemination of Escherichia coli Sequence Type 410 Closely Related to B4/H24RxC in Ghana
description	Extra-intestinal pathogenic Escherichia coli (ExPEC) is one of the world’s leading causes of bloodstream infections with high mortality. Sequence type 410 (ST410) is an emerging ExPEC clone resistant to a wide range of antibiotics. In this study, we investigated the epidemiology of 21 ST410 E. coli isolates from two Ghanaian hospitals. We also investigated the isolates within a global context to provide further insight into the dissemination of this highly pathogenic clone. A phylogenetic tree of the 21 isolate genomes, along with 102 others from global collection, was constructed representing the ensuing clades and sub-clades of the ST: A/H53, B2/H24R, B3/H24Rx, and B4/H24RxC. The carbapenem-resistant sub-clade B4/H24RxC is reported to have emerged in the early 2000s when ST410 acquired an IncX3 plasmid carrying a blaOXA–181 carbapenemase gene, and a second carbapenemase gene, blaNDM–5, on a conserved IncFII plasmid in 2014. We identified, in this study, one blaOXA–181–carrying isolate belonging to B4/H24RxC sub-lineage and one carrying blaNDM–1 belonging to sub-lineage B3/H24Rx. The blaOXA–181 gene was found on a 51kb IncX3 plasmid; pEc1079_3. The majority (12/21) of our Ghanaian isolates were clustered with international strains described by previous authors as closely related strains to B4/H24RxC. Six others were clustered among the ESBL-associated sub-lineage B3/H24Rx and three with the globally disseminated sub-lineage B4/H24RxC. The results show that this highly pathogenic clone is disseminated in Ghana and, given its ability to transmit between hosts, it poses a serious threat and should be monitored closely.
format	article
author	Samiratu Mahazu Samiratu Mahazu Isaac Prah Isaac Prah Alafate Ayibieke Wakana Sato Takaya Hayashi Toshihiko Suzuki Shiroh Iwanaga Anthony Ablordey Ryoichi Saito
author_facet	Samiratu Mahazu Samiratu Mahazu Isaac Prah Isaac Prah Alafate Ayibieke Wakana Sato Takaya Hayashi Toshihiko Suzuki Shiroh Iwanaga Anthony Ablordey Ryoichi Saito
author_sort	Samiratu Mahazu
title	Possible Dissemination of Escherichia coli Sequence Type 410 Closely Related to B4/H24RxC in Ghana
title_short	Possible Dissemination of Escherichia coli Sequence Type 410 Closely Related to B4/H24RxC in Ghana
title_full	Possible Dissemination of Escherichia coli Sequence Type 410 Closely Related to B4/H24RxC in Ghana
title_fullStr	Possible Dissemination of Escherichia coli Sequence Type 410 Closely Related to B4/H24RxC in Ghana
title_full_unstemmed	Possible Dissemination of Escherichia coli Sequence Type 410 Closely Related to B4/H24RxC in Ghana
title_sort	possible dissemination of escherichia coli sequence type 410 closely related to b4/h24rxc in ghana
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/c42ca45a6467457a960cc236579368b2
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Possible Dissemination of Escherichia coli Sequence Type 410 Closely Related to B4/H24RxC in Ghana

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