Novel Immunoinformatics Approaches to Design Multi-epitope Subunit Vaccine for Malaria by Investigating Anopheles Salivary Protein

Abstract Malaria fever has been pervasive for quite a while in tropical developing regions causing high morbidity and mortality. The causal organism is a protozoan parasite of genus Plasmodium which spreads to the human host by the bite of hitherto infected female Anopheles mosquito. In the course o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rajan Kumar Pandey, Tarun Kumar Bhatt, Vijay Kumar Prajapati
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c42db7f5485d4f1ba62061a39a9ea9f6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c42db7f5485d4f1ba62061a39a9ea9f6
record_format	dspace
spelling	oai:doaj.org-article:c42db7f5485d4f1ba62061a39a9ea9f62021-12-02T11:40:36ZNovel Immunoinformatics Approaches to Design Multi-epitope Subunit Vaccine for Malaria by Investigating Anopheles Salivary Protein10.1038/s41598-018-19456-12045-2322https://doaj.org/article/c42db7f5485d4f1ba62061a39a9ea9f62018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19456-1https://doaj.org/toc/2045-2322Abstract Malaria fever has been pervasive for quite a while in tropical developing regions causing high morbidity and mortality. The causal organism is a protozoan parasite of genus Plasmodium which spreads to the human host by the bite of hitherto infected female Anopheles mosquito. In the course of biting, a salivary protein of Anopheles helps in blood feeding behavior and having the ability to elicit the host immune response. This study represents a series of immunoinformatics approaches to design multi-epitope subunit vaccine using Anopheles mosquito salivary proteins. Designed subunit vaccine was evaluated for its immunogenicity, allergenicity and physiochemical parameters. To enhance the stability of vaccine protein, disulfide engineering was performed in a region of high mobility. Codon adaptation and in silico cloning was also performed to ensure the higher expression of designed subunit vaccine in E. coli K12 expression system. Finally, molecular docking and simulation study was performed for the vaccine protein and TLR-4 receptor, to determine the binding free energy and complex stability. Moreover, the designed subunit vaccine was found to induce anti-salivary immunity which may have the ability to prevent the entry of Plasmodium sporozoites into the human host.Rajan Kumar PandeyTarun Kumar BhattVijay Kumar PrajapatiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rajan Kumar Pandey Tarun Kumar Bhatt Vijay Kumar Prajapati Novel Immunoinformatics Approaches to Design Multi-epitope Subunit Vaccine for Malaria by Investigating Anopheles Salivary Protein
description	Abstract Malaria fever has been pervasive for quite a while in tropical developing regions causing high morbidity and mortality. The causal organism is a protozoan parasite of genus Plasmodium which spreads to the human host by the bite of hitherto infected female Anopheles mosquito. In the course of biting, a salivary protein of Anopheles helps in blood feeding behavior and having the ability to elicit the host immune response. This study represents a series of immunoinformatics approaches to design multi-epitope subunit vaccine using Anopheles mosquito salivary proteins. Designed subunit vaccine was evaluated for its immunogenicity, allergenicity and physiochemical parameters. To enhance the stability of vaccine protein, disulfide engineering was performed in a region of high mobility. Codon adaptation and in silico cloning was also performed to ensure the higher expression of designed subunit vaccine in E. coli K12 expression system. Finally, molecular docking and simulation study was performed for the vaccine protein and TLR-4 receptor, to determine the binding free energy and complex stability. Moreover, the designed subunit vaccine was found to induce anti-salivary immunity which may have the ability to prevent the entry of Plasmodium sporozoites into the human host.
format	article
author	Rajan Kumar Pandey Tarun Kumar Bhatt Vijay Kumar Prajapati
author_facet	Rajan Kumar Pandey Tarun Kumar Bhatt Vijay Kumar Prajapati
author_sort	Rajan Kumar Pandey
title	Novel Immunoinformatics Approaches to Design Multi-epitope Subunit Vaccine for Malaria by Investigating Anopheles Salivary Protein
title_short	Novel Immunoinformatics Approaches to Design Multi-epitope Subunit Vaccine for Malaria by Investigating Anopheles Salivary Protein
title_full	Novel Immunoinformatics Approaches to Design Multi-epitope Subunit Vaccine for Malaria by Investigating Anopheles Salivary Protein
title_fullStr	Novel Immunoinformatics Approaches to Design Multi-epitope Subunit Vaccine for Malaria by Investigating Anopheles Salivary Protein
title_full_unstemmed	Novel Immunoinformatics Approaches to Design Multi-epitope Subunit Vaccine for Malaria by Investigating Anopheles Salivary Protein
title_sort	novel immunoinformatics approaches to design multi-epitope subunit vaccine for malaria by investigating anopheles salivary protein
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/c42db7f5485d4f1ba62061a39a9ea9f6
work_keys_str_mv	AT rajankumarpandey novelimmunoinformaticsapproachestodesignmultiepitopesubunitvaccineformalariabyinvestigatinganophelessalivaryprotein AT tarunkumarbhatt novelimmunoinformaticsapproachestodesignmultiepitopesubunitvaccineformalariabyinvestigatinganophelessalivaryprotein AT vijaykumarprajapati novelimmunoinformaticsapproachestodesignmultiepitopesubunitvaccineformalariabyinvestigatinganophelessalivaryprotein
_version_	1718395604178042880

Novel Immunoinformatics Approaches to Design Multi-epitope Subunit Vaccine for Malaria by Investigating Anopheles Salivary Protein

Ejemplares similares