Distribution pattern and molecular signature of cholinergic tuft cells in human gastro-intestinal and pancreatic-biliary tract

Abstract Despite considerable recent insight into the molecular phenotypes and type 2 innate immune functions of tuft cells in rodents, there is sparse knowledge about the region-specific presence and molecular phenotypes of tuft cells in the human digestive tract. Here, we traced cholinergic tuft c...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Burkhard Schütz, Anna-Lena Ruppert, Oliver Strobel, Michael Lazarus, Yoshihiro Urade, Markus W. Büchler, Eberhard Weihe
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c432bbd8a8604289aa9dc0f6fac71f61
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c432bbd8a8604289aa9dc0f6fac71f61
record_format	dspace
spelling	oai:doaj.org-article:c432bbd8a8604289aa9dc0f6fac71f612021-12-02T15:08:20ZDistribution pattern and molecular signature of cholinergic tuft cells in human gastro-intestinal and pancreatic-biliary tract10.1038/s41598-019-53997-32045-2322https://doaj.org/article/c432bbd8a8604289aa9dc0f6fac71f612019-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-53997-3https://doaj.org/toc/2045-2322Abstract Despite considerable recent insight into the molecular phenotypes and type 2 innate immune functions of tuft cells in rodents, there is sparse knowledge about the region-specific presence and molecular phenotypes of tuft cells in the human digestive tract. Here, we traced cholinergic tuft cells throughout the human alimentary tract with immunohistochemistry and deciphered their region-specific distribution and biomolecule coexistence patterns. While absent from the human stomach, cholinergic tuft cells localized to villi and crypts in the small and large intestines. In the biliary tract, they were present in the epithelium of extra-hepatic peribiliary glands, but not observed in the epithelia of the gall bladder and the common duct of the biliary tract. In the pancreas, solitary cholinergic tuft cells were frequently observed in the epithelia of small and medium-size intra- and inter-lobular ducts, while they were absent from acinar cells and from the main pancreatic duct. Double immunofluorescence revealed co-expression of choline acetyltransferase with structural (cytokeratin 18, villin, advillin) tuft cell markers and eicosanoid signaling (cyclooxygenase 1, hematopoietic prostaglandin D synthase, 5-lipoxygenase activating protein) biomolecules. Our results indicate that region-specific cholinergic signaling of tuft cells plays a role in mucosal immunity in health and disease, especially in infection and cancer.Burkhard SchützAnna-Lena RuppertOliver StrobelMichael LazarusYoshihiro UradeMarkus W. BüchlerEberhard WeiheNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Burkhard Schütz Anna-Lena Ruppert Oliver Strobel Michael Lazarus Yoshihiro Urade Markus W. Büchler Eberhard Weihe Distribution pattern and molecular signature of cholinergic tuft cells in human gastro-intestinal and pancreatic-biliary tract
description	Abstract Despite considerable recent insight into the molecular phenotypes and type 2 innate immune functions of tuft cells in rodents, there is sparse knowledge about the region-specific presence and molecular phenotypes of tuft cells in the human digestive tract. Here, we traced cholinergic tuft cells throughout the human alimentary tract with immunohistochemistry and deciphered their region-specific distribution and biomolecule coexistence patterns. While absent from the human stomach, cholinergic tuft cells localized to villi and crypts in the small and large intestines. In the biliary tract, they were present in the epithelium of extra-hepatic peribiliary glands, but not observed in the epithelia of the gall bladder and the common duct of the biliary tract. In the pancreas, solitary cholinergic tuft cells were frequently observed in the epithelia of small and medium-size intra- and inter-lobular ducts, while they were absent from acinar cells and from the main pancreatic duct. Double immunofluorescence revealed co-expression of choline acetyltransferase with structural (cytokeratin 18, villin, advillin) tuft cell markers and eicosanoid signaling (cyclooxygenase 1, hematopoietic prostaglandin D synthase, 5-lipoxygenase activating protein) biomolecules. Our results indicate that region-specific cholinergic signaling of tuft cells plays a role in mucosal immunity in health and disease, especially in infection and cancer.
format	article
author	Burkhard Schütz Anna-Lena Ruppert Oliver Strobel Michael Lazarus Yoshihiro Urade Markus W. Büchler Eberhard Weihe
author_facet	Burkhard Schütz Anna-Lena Ruppert Oliver Strobel Michael Lazarus Yoshihiro Urade Markus W. Büchler Eberhard Weihe
author_sort	Burkhard Schütz
title	Distribution pattern and molecular signature of cholinergic tuft cells in human gastro-intestinal and pancreatic-biliary tract
title_short	Distribution pattern and molecular signature of cholinergic tuft cells in human gastro-intestinal and pancreatic-biliary tract
title_full	Distribution pattern and molecular signature of cholinergic tuft cells in human gastro-intestinal and pancreatic-biliary tract
title_fullStr	Distribution pattern and molecular signature of cholinergic tuft cells in human gastro-intestinal and pancreatic-biliary tract
title_full_unstemmed	Distribution pattern and molecular signature of cholinergic tuft cells in human gastro-intestinal and pancreatic-biliary tract
title_sort	distribution pattern and molecular signature of cholinergic tuft cells in human gastro-intestinal and pancreatic-biliary tract
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/c432bbd8a8604289aa9dc0f6fac71f61
work_keys_str_mv	AT burkhardschutz distributionpatternandmolecularsignatureofcholinergictuftcellsinhumangastrointestinalandpancreaticbiliarytract AT annalenaruppert distributionpatternandmolecularsignatureofcholinergictuftcellsinhumangastrointestinalandpancreaticbiliarytract AT oliverstrobel distributionpatternandmolecularsignatureofcholinergictuftcellsinhumangastrointestinalandpancreaticbiliarytract AT michaellazarus distributionpatternandmolecularsignatureofcholinergictuftcellsinhumangastrointestinalandpancreaticbiliarytract AT yoshihirourade distributionpatternandmolecularsignatureofcholinergictuftcellsinhumangastrointestinalandpancreaticbiliarytract AT markuswbuchler distributionpatternandmolecularsignatureofcholinergictuftcellsinhumangastrointestinalandpancreaticbiliarytract AT eberhardweihe distributionpatternandmolecularsignatureofcholinergictuftcellsinhumangastrointestinalandpancreaticbiliarytract
_version_	1718388212058030080

Distribution pattern and molecular signature of cholinergic tuft cells in human gastro-intestinal and pancreatic-biliary tract

Ejemplares similares