Integrated Analysis of the Metabolome and Transcriptome on Anthocyanin Biosynthesis in Four Developmental Stages of <i>Cerasus humilis</i> Peel Coloration
<i>Cerasus humilis</i> is a unique dwarf shrub and fruit color is an important trait in the species. In this study, we evaluated the transcriptomic and metabolomic profiles of the plant at different developmental stages to elucidate the mechanism underlying color formation. In a metabolo...
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/c433f7b6578244f2ba0414b253a27e02 |
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| Sumario: | <i>Cerasus humilis</i> is a unique dwarf shrub and fruit color is an important trait in the species. In this study, we evaluated the transcriptomic and metabolomic profiles of the plant at different developmental stages to elucidate the mechanism underlying color formation. In a metabolomics analysis, 16 anthocyanin components were identified at four developmental stages, and high levels of cyanidin <i>O</i>-syringic acid and pelargonidin 3-<i>O</i>-beta-<span style="font-variant: small-caps;">d</span>-glucoside (callitephin chloride) were correlated with the reddening of the fruit peel. A co-expression analysis revealed that <i>ANS</i> and <i>UFGT</i> play key roles in pigmentation (PCC > 0.82). Additionally, transcriptome data showed that most anthocyanin biosynthetic genes and two MYB transcription factors were significantly up-regulated. QRT-PCR results for these differentially expressed genes were generally consistent with the high-throughput sequencing. Moreover, the overexpression of <i>ChMYB1</i> (TRINITY_DN21536_c0_g1) in apple calli could contribute to the accumulation of anthocyanin. It was also found that <i>UFGT</i> (TRINITY_DN19893_c1_g5) and <i>ChMYB1</i> (TRINITY_DN21536_c0_g1) have similar expression patterns. These findings provide insight into the mechanisms underlying anthocyanin accumulation and coloration during fruit peel development, providing a basis for the breeding of anthocyanin-rich <i>C. humilis</i> cultivars. |
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