Integrated Analysis of the Metabolome and Transcriptome on Anthocyanin Biosynthesis in Four Developmental Stages of <i>Cerasus humilis</i> Peel Coloration

<i>Cerasus humilis</i> is a unique dwarf shrub and fruit color is an important trait in the species. In this study, we evaluated the transcriptomic and metabolomic profiles of the plant at different developmental stages to elucidate the mechanism underlying color formation. In a metabolo...

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Autores principales:	Xiaolong Ji, Jing Ren, Yixin Zhang, Shaoyu Lang, Di Wang, Xingshun Song
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Cerasus humilis</i> anthocyanin pigmentation transcriptome metabolites Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/c433f7b6578244f2ba0414b253a27e02
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spelling	oai:doaj.org-article:c433f7b6578244f2ba0414b253a27e022021-11-11T17:18:06ZIntegrated Analysis of the Metabolome and Transcriptome on Anthocyanin Biosynthesis in Four Developmental Stages of <i>Cerasus humilis</i> Peel Coloration10.3390/ijms2221118801422-00671661-6596https://doaj.org/article/c433f7b6578244f2ba0414b253a27e022021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11880https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067<i>Cerasus humilis</i> is a unique dwarf shrub and fruit color is an important trait in the species. In this study, we evaluated the transcriptomic and metabolomic profiles of the plant at different developmental stages to elucidate the mechanism underlying color formation. In a metabolomics analysis, 16 anthocyanin components were identified at four developmental stages, and high levels of cyanidin <i>O</i>-syringic acid and pelargonidin 3-<i>O</i>-beta-<span style="font-variant: small-caps;">d</span>-glucoside (callitephin chloride) were correlated with the reddening of the fruit peel. A co-expression analysis revealed that <i>ANS</i> and <i>UFGT</i> play key roles in pigmentation (PCC > 0.82). Additionally, transcriptome data showed that most anthocyanin biosynthetic genes and two MYB transcription factors were significantly up-regulated. QRT-PCR results for these differentially expressed genes were generally consistent with the high-throughput sequencing. Moreover, the overexpression of <i>ChMYB1</i> (TRINITY_DN21536_c0_g1) in apple calli could contribute to the accumulation of anthocyanin. It was also found that <i>UFGT</i> (TRINITY_DN19893_c1_g5) and <i>ChMYB1</i> (TRINITY_DN21536_c0_g1) have similar expression patterns. These findings provide insight into the mechanisms underlying anthocyanin accumulation and coloration during fruit peel development, providing a basis for the breeding of anthocyanin-rich <i>C. humilis</i> cultivars.Xiaolong JiJing RenYixin ZhangShaoyu LangDi WangXingshun SongMDPI AGarticle<i>Cerasus humilis</i>anthocyaninpigmentationtranscriptomemetabolitesBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11880, p 11880 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Cerasus humilis</i> anthocyanin pigmentation transcriptome metabolites Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Cerasus humilis</i> anthocyanin pigmentation transcriptome metabolites Biology (General) QH301-705.5 Chemistry QD1-999 Xiaolong Ji Jing Ren Yixin Zhang Shaoyu Lang Di Wang Xingshun Song Integrated Analysis of the Metabolome and Transcriptome on Anthocyanin Biosynthesis in Four Developmental Stages of <i>Cerasus humilis</i> Peel Coloration
description	<i>Cerasus humilis</i> is a unique dwarf shrub and fruit color is an important trait in the species. In this study, we evaluated the transcriptomic and metabolomic profiles of the plant at different developmental stages to elucidate the mechanism underlying color formation. In a metabolomics analysis, 16 anthocyanin components were identified at four developmental stages, and high levels of cyanidin <i>O</i>-syringic acid and pelargonidin 3-<i>O</i>-beta-<span style="font-variant: small-caps;">d</span>-glucoside (callitephin chloride) were correlated with the reddening of the fruit peel. A co-expression analysis revealed that <i>ANS</i> and <i>UFGT</i> play key roles in pigmentation (PCC > 0.82). Additionally, transcriptome data showed that most anthocyanin biosynthetic genes and two MYB transcription factors were significantly up-regulated. QRT-PCR results for these differentially expressed genes were generally consistent with the high-throughput sequencing. Moreover, the overexpression of <i>ChMYB1</i> (TRINITY_DN21536_c0_g1) in apple calli could contribute to the accumulation of anthocyanin. It was also found that <i>UFGT</i> (TRINITY_DN19893_c1_g5) and <i>ChMYB1</i> (TRINITY_DN21536_c0_g1) have similar expression patterns. These findings provide insight into the mechanisms underlying anthocyanin accumulation and coloration during fruit peel development, providing a basis for the breeding of anthocyanin-rich <i>C. humilis</i> cultivars.
format	article
author	Xiaolong Ji Jing Ren Yixin Zhang Shaoyu Lang Di Wang Xingshun Song
author_facet	Xiaolong Ji Jing Ren Yixin Zhang Shaoyu Lang Di Wang Xingshun Song
author_sort	Xiaolong Ji
title	Integrated Analysis of the Metabolome and Transcriptome on Anthocyanin Biosynthesis in Four Developmental Stages of <i>Cerasus humilis</i> Peel Coloration
title_short	Integrated Analysis of the Metabolome and Transcriptome on Anthocyanin Biosynthesis in Four Developmental Stages of <i>Cerasus humilis</i> Peel Coloration
title_full	Integrated Analysis of the Metabolome and Transcriptome on Anthocyanin Biosynthesis in Four Developmental Stages of <i>Cerasus humilis</i> Peel Coloration
title_fullStr	Integrated Analysis of the Metabolome and Transcriptome on Anthocyanin Biosynthesis in Four Developmental Stages of <i>Cerasus humilis</i> Peel Coloration
title_full_unstemmed	Integrated Analysis of the Metabolome and Transcriptome on Anthocyanin Biosynthesis in Four Developmental Stages of <i>Cerasus humilis</i> Peel Coloration
title_sort	integrated analysis of the metabolome and transcriptome on anthocyanin biosynthesis in four developmental stages of <i>cerasus humilis</i> peel coloration
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c433f7b6578244f2ba0414b253a27e02
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Integrated Analysis of the Metabolome and Transcriptome on Anthocyanin Biosynthesis in Four Developmental Stages of <i>Cerasus humilis</i> Peel Coloration

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