Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions

Abstract Multiphoton excitation microscopy (MPM) is regarded as an effective tool that enables the visualization of deep regions within living tissues and organs, with little damage. Here, we report novel non-labeling MPM (NL-MPM) imaging of fresh human colorectal mucosa, which is useful for discrim...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Takahiro Matsui, Hiroki Mizuno, Takao Sudo, Junichi Kikuta, Naotsugu Haraguchi, Jun-ichiro Ikeda, Tsunekazu Mizushima, Hirofumi Yamamoto, Eiichi Morii, Masaki Mori, Masaru Ishii
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c43ac4bddec64a5baad9827194f4cbb0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c43ac4bddec64a5baad9827194f4cbb0
record_format	dspace
spelling	oai:doaj.org-article:c43ac4bddec64a5baad9827194f4cbb02021-12-02T11:52:21ZNon-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions10.1038/s41598-017-07244-22045-2322https://doaj.org/article/c43ac4bddec64a5baad9827194f4cbb02017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07244-2https://doaj.org/toc/2045-2322Abstract Multiphoton excitation microscopy (MPM) is regarded as an effective tool that enables the visualization of deep regions within living tissues and organs, with little damage. Here, we report novel non-labeling MPM (NL-MPM) imaging of fresh human colorectal mucosa, which is useful for discriminating cancer lesions from normal tissues quantitatively without any need for resection, fixation, or staining. Using NL-MPM, we visualized three components in human colorectal mucosa, epithelial cells, immune cells, and basement membranes, based on their characteristic patterns of fluorescence. These patterns are characterized by the different auto-fluorescence properties of nicotinamide adenine dinucleotide, nicotinamide adenine dinucleotide phosphate, and flavin adenine dinucleotide and from second harmonic generation (SHG). NL-MPM images were at least as informative to pathologists as were ‘conventional’ images of fixed tissue sections stained with hematoxylin and eosin. Additionally, two quantitative parameters extracted from NL-MPM images – the nucleus diameter (index N) and the intensity of SHG in the basement membrane (index S) – rendered it possible to diagnose cancer regions effectively. In conclusion, NL-MPM is a novel, promising method for real-time clinical diagnosis of colorectal cancers, and is associated with minimal invasiveness.Takahiro MatsuiHiroki MizunoTakao SudoJunichi KikutaNaotsugu HaraguchiJun-ichiro IkedaTsunekazu MizushimaHirofumi YamamotoEiichi MoriiMasaki MoriMasaru IshiiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takahiro Matsui Hiroki Mizuno Takao Sudo Junichi Kikuta Naotsugu Haraguchi Jun-ichiro Ikeda Tsunekazu Mizushima Hirofumi Yamamoto Eiichi Morii Masaki Mori Masaru Ishii Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions
description	Abstract Multiphoton excitation microscopy (MPM) is regarded as an effective tool that enables the visualization of deep regions within living tissues and organs, with little damage. Here, we report novel non-labeling MPM (NL-MPM) imaging of fresh human colorectal mucosa, which is useful for discriminating cancer lesions from normal tissues quantitatively without any need for resection, fixation, or staining. Using NL-MPM, we visualized three components in human colorectal mucosa, epithelial cells, immune cells, and basement membranes, based on their characteristic patterns of fluorescence. These patterns are characterized by the different auto-fluorescence properties of nicotinamide adenine dinucleotide, nicotinamide adenine dinucleotide phosphate, and flavin adenine dinucleotide and from second harmonic generation (SHG). NL-MPM images were at least as informative to pathologists as were ‘conventional’ images of fixed tissue sections stained with hematoxylin and eosin. Additionally, two quantitative parameters extracted from NL-MPM images – the nucleus diameter (index N) and the intensity of SHG in the basement membrane (index S) – rendered it possible to diagnose cancer regions effectively. In conclusion, NL-MPM is a novel, promising method for real-time clinical diagnosis of colorectal cancers, and is associated with minimal invasiveness.
format	article
author	Takahiro Matsui Hiroki Mizuno Takao Sudo Junichi Kikuta Naotsugu Haraguchi Jun-ichiro Ikeda Tsunekazu Mizushima Hirofumi Yamamoto Eiichi Morii Masaki Mori Masaru Ishii
author_facet	Takahiro Matsui Hiroki Mizuno Takao Sudo Junichi Kikuta Naotsugu Haraguchi Jun-ichiro Ikeda Tsunekazu Mizushima Hirofumi Yamamoto Eiichi Morii Masaki Mori Masaru Ishii
author_sort	Takahiro Matsui
title	Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions
title_short	Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions
title_full	Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions
title_fullStr	Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions
title_full_unstemmed	Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions
title_sort	non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c43ac4bddec64a5baad9827194f4cbb0
work_keys_str_mv	AT takahiromatsui nonlabelingmultiphotonexcitationmicroscopyasanoveldiagnostictoolfordiscriminatingnormaltissueandcolorectalcancerlesions AT hirokimizuno nonlabelingmultiphotonexcitationmicroscopyasanoveldiagnostictoolfordiscriminatingnormaltissueandcolorectalcancerlesions AT takaosudo nonlabelingmultiphotonexcitationmicroscopyasanoveldiagnostictoolfordiscriminatingnormaltissueandcolorectalcancerlesions AT junichikikuta nonlabelingmultiphotonexcitationmicroscopyasanoveldiagnostictoolfordiscriminatingnormaltissueandcolorectalcancerlesions AT naotsuguharaguchi nonlabelingmultiphotonexcitationmicroscopyasanoveldiagnostictoolfordiscriminatingnormaltissueandcolorectalcancerlesions AT junichiroikeda nonlabelingmultiphotonexcitationmicroscopyasanoveldiagnostictoolfordiscriminatingnormaltissueandcolorectalcancerlesions AT tsunekazumizushima nonlabelingmultiphotonexcitationmicroscopyasanoveldiagnostictoolfordiscriminatingnormaltissueandcolorectalcancerlesions AT hirofumiyamamoto nonlabelingmultiphotonexcitationmicroscopyasanoveldiagnostictoolfordiscriminatingnormaltissueandcolorectalcancerlesions AT eiichimorii nonlabelingmultiphotonexcitationmicroscopyasanoveldiagnostictoolfordiscriminatingnormaltissueandcolorectalcancerlesions AT masakimori nonlabelingmultiphotonexcitationmicroscopyasanoveldiagnostictoolfordiscriminatingnormaltissueandcolorectalcancerlesions AT masaruishii nonlabelingmultiphotonexcitationmicroscopyasanoveldiagnostictoolfordiscriminatingnormaltissueandcolorectalcancerlesions
_version_	1718395068703834112

Non-labeling multiphoton excitation microscopy as a novel diagnostic tool for discriminating normal tissue and colorectal cancer lesions

Ejemplares similares