Blue light directly modulates the quorum network in the human pathogen Acinetobacter baumannii

Abstract Quorum sensing modulates bacterial collective behaviors including biofilm formation, motility and virulence in the important human pathogen Acinetobacter baumannii. Disruption of quorum sensing has emerged as a promising strategy with important therapeutic potential. In this work, we show t...

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Autores principales:	Marisel Romina Tuttobene, Gabriela Leticia Müller, Lucía Blasco, Natalia Arana, Mónica Hourcade, Lautaro Diacovich, Pamela Cribb, María Tomás, Carlos Gabriel Nieto-Peñalver, María Alejandra Mussi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c4427070d1c84c61970490e3176406bc
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spelling	oai:doaj.org-article:c4427070d1c84c61970490e3176406bc2021-12-02T16:31:46ZBlue light directly modulates the quorum network in the human pathogen Acinetobacter baumannii10.1038/s41598-021-92845-12045-2322https://doaj.org/article/c4427070d1c84c61970490e3176406bc2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92845-1https://doaj.org/toc/2045-2322Abstract Quorum sensing modulates bacterial collective behaviors including biofilm formation, motility and virulence in the important human pathogen Acinetobacter baumannii. Disruption of quorum sensing has emerged as a promising strategy with important therapeutic potential. In this work, we show that light modulates the production of acyl-homoserine lactones (AHLs), which were produced in higher levels in the dark than under blue light at environmental temperatures, a response that depends on the AHL synthase, AbaI, and on the photoreceptor BlsA. BlsA interacts with the transcriptional regulator AbaR in the dark at environmental temperatures, inducing abaI expression. Under blue light, BlsA does not interact with AbaR, but induces expression of the lactonase aidA and quorum quenching, consistently with lack of motility at this condition. At temperatures found in warm-blooded hosts, the production of AHLs, quorum quenching as well as abaI and aidA expression were also modulated by light, though in this case higher levels of AHLs were detected under blue light than in the dark, in a BlsA-independent manner. Finally, AbaI reduces A. baumannii's ability to kill C. albicans only in the dark both at environmental as well as at temperatures found in warm-blooded hosts. The overall data indicate that light directly modulates quorum network in A. baumannii.Marisel Romina TuttobeneGabriela Leticia MüllerLucía BlascoNatalia AranaMónica HourcadeLautaro DiacovichPamela CribbMaría TomásCarlos Gabriel Nieto-PeñalverMaría Alejandra MussiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-20 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marisel Romina Tuttobene Gabriela Leticia Müller Lucía Blasco Natalia Arana Mónica Hourcade Lautaro Diacovich Pamela Cribb María Tomás Carlos Gabriel Nieto-Peñalver María Alejandra Mussi Blue light directly modulates the quorum network in the human pathogen Acinetobacter baumannii
description	Abstract Quorum sensing modulates bacterial collective behaviors including biofilm formation, motility and virulence in the important human pathogen Acinetobacter baumannii. Disruption of quorum sensing has emerged as a promising strategy with important therapeutic potential. In this work, we show that light modulates the production of acyl-homoserine lactones (AHLs), which were produced in higher levels in the dark than under blue light at environmental temperatures, a response that depends on the AHL synthase, AbaI, and on the photoreceptor BlsA. BlsA interacts with the transcriptional regulator AbaR in the dark at environmental temperatures, inducing abaI expression. Under blue light, BlsA does not interact with AbaR, but induces expression of the lactonase aidA and quorum quenching, consistently with lack of motility at this condition. At temperatures found in warm-blooded hosts, the production of AHLs, quorum quenching as well as abaI and aidA expression were also modulated by light, though in this case higher levels of AHLs were detected under blue light than in the dark, in a BlsA-independent manner. Finally, AbaI reduces A. baumannii's ability to kill C. albicans only in the dark both at environmental as well as at temperatures found in warm-blooded hosts. The overall data indicate that light directly modulates quorum network in A. baumannii.
format	article
author	Marisel Romina Tuttobene Gabriela Leticia Müller Lucía Blasco Natalia Arana Mónica Hourcade Lautaro Diacovich Pamela Cribb María Tomás Carlos Gabriel Nieto-Peñalver María Alejandra Mussi
author_facet	Marisel Romina Tuttobene Gabriela Leticia Müller Lucía Blasco Natalia Arana Mónica Hourcade Lautaro Diacovich Pamela Cribb María Tomás Carlos Gabriel Nieto-Peñalver María Alejandra Mussi
author_sort	Marisel Romina Tuttobene
title	Blue light directly modulates the quorum network in the human pathogen Acinetobacter baumannii
title_short	Blue light directly modulates the quorum network in the human pathogen Acinetobacter baumannii
title_full	Blue light directly modulates the quorum network in the human pathogen Acinetobacter baumannii
title_fullStr	Blue light directly modulates the quorum network in the human pathogen Acinetobacter baumannii
title_full_unstemmed	Blue light directly modulates the quorum network in the human pathogen Acinetobacter baumannii
title_sort	blue light directly modulates the quorum network in the human pathogen acinetobacter baumannii
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c4427070d1c84c61970490e3176406bc
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Blue light directly modulates the quorum network in the human pathogen Acinetobacter baumannii

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