Adipose‐Derived Stromal Cells Are Capable of Restoring Bone Regeneration After Post‐Traumatic Osteomyelitis and Modulate B‐Cell Response

Abstract Bone infections are a frequent cause for large bony defects with a reduced healing capacity. In previous findings, we could already show diminished healing capacity after bone infections, despite the absence of the causing agent, Staphylococcus aureus. Moreover, these bony defects showed re...

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Autores principales:	Johannes Maximilian Wagner, Felix Reinkemeier, Christoph Wallner, Mehran Dadras, Julika Huber, Sonja Verena Schmidt, Marius Drysch, Stephanie Dittfeld, Henriette Jaurich, Mustafa Becerikli, Kathrin Becker, Nicole Rauch, Vikas Duhan, Marcus Lehnhardt, Björn Behr
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2019
Materias:	Post‐traumatic osteomyelitis Bone regeneration Stem cells B‐cells Osteoimmunology Medicine (General) R5-920 Cytology QH573-671
Acceso en línea:	https://doaj.org/article/c4442bc71f8d4b39976ad6bc477dcbf6
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spelling	oai:doaj.org-article:c4442bc71f8d4b39976ad6bc477dcbf62021-11-30T19:15:37ZAdipose‐Derived Stromal Cells Are Capable of Restoring Bone Regeneration After Post‐Traumatic Osteomyelitis and Modulate B‐Cell Response2157-65802157-656410.1002/sctm.18-0266https://doaj.org/article/c4442bc71f8d4b39976ad6bc477dcbf62019-10-01T00:00:00Zhttps://doi.org/10.1002/sctm.18-0266https://doaj.org/toc/2157-6564https://doaj.org/toc/2157-6580Abstract Bone infections are a frequent cause for large bony defects with a reduced healing capacity. In previous findings, we could already show diminished healing capacity after bone infections, despite the absence of the causing agent, Staphylococcus aureus. Moreover, these bony defects showed reduced osteoblastogenesis and increased osteoclastogenesis, meaning elevated bone resorption ongoing with an elevated B‐cell activity. To overcome the negative effects of this postinfectious inflammatory state, we tried to use the regenerative capacity of mesenchymal stem cells derived from adipose tissue (adipose‐derived stem cells [ASCs]) to improve bone regeneration and moreover were curious about immunomodulation of applicated stem cells in this setting. Therefore, we used our established murine animal model and applicated ASCs locally after sufficient debridement of infected bones. Bone regeneration and resorption as well as immunological markers were investigated via histology, immunohistochemistry, Western blot, and fluorescence‐activated cell scanning (FACS) analysis and μ‐computed tomography (CT) analysis. Interestingly, ASCs were able to restore bone healing via elevation of osteoblastogenesis and downregulation of osteoclasts. Surprisingly, stem cells showed an impact on the innate immune system, downregulating B‐cell population. In summary, these data provide a fascinating new and innovative approach, supporting bone healing after bacterial infections and moreover gain insights into the complex ceremony of stem cell interaction in terms of bone infection and regeneration. Stem Cells Translational Medicine 2019;8:1084–1091Johannes Maximilian WagnerFelix ReinkemeierChristoph WallnerMehran DadrasJulika HuberSonja Verena SchmidtMarius DryschStephanie DittfeldHenriette JaurichMustafa BecerikliKathrin BeckerNicole RauchVikas DuhanMarcus LehnhardtBjörn BehrWileyarticlePost‐traumatic osteomyelitisBone regenerationStem cellsB‐cellsOsteoimmunologyMedicine (General)R5-920CytologyQH573-671ENStem Cells Translational Medicine, Vol 8, Iss 10, Pp 1084-1091 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Post‐traumatic osteomyelitis Bone regeneration Stem cells B‐cells Osteoimmunology Medicine (General) R5-920 Cytology QH573-671
spellingShingle	Post‐traumatic osteomyelitis Bone regeneration Stem cells B‐cells Osteoimmunology Medicine (General) R5-920 Cytology QH573-671 Johannes Maximilian Wagner Felix Reinkemeier Christoph Wallner Mehran Dadras Julika Huber Sonja Verena Schmidt Marius Drysch Stephanie Dittfeld Henriette Jaurich Mustafa Becerikli Kathrin Becker Nicole Rauch Vikas Duhan Marcus Lehnhardt Björn Behr Adipose‐Derived Stromal Cells Are Capable of Restoring Bone Regeneration After Post‐Traumatic Osteomyelitis and Modulate B‐Cell Response
description	Abstract Bone infections are a frequent cause for large bony defects with a reduced healing capacity. In previous findings, we could already show diminished healing capacity after bone infections, despite the absence of the causing agent, Staphylococcus aureus. Moreover, these bony defects showed reduced osteoblastogenesis and increased osteoclastogenesis, meaning elevated bone resorption ongoing with an elevated B‐cell activity. To overcome the negative effects of this postinfectious inflammatory state, we tried to use the regenerative capacity of mesenchymal stem cells derived from adipose tissue (adipose‐derived stem cells [ASCs]) to improve bone regeneration and moreover were curious about immunomodulation of applicated stem cells in this setting. Therefore, we used our established murine animal model and applicated ASCs locally after sufficient debridement of infected bones. Bone regeneration and resorption as well as immunological markers were investigated via histology, immunohistochemistry, Western blot, and fluorescence‐activated cell scanning (FACS) analysis and μ‐computed tomography (CT) analysis. Interestingly, ASCs were able to restore bone healing via elevation of osteoblastogenesis and downregulation of osteoclasts. Surprisingly, stem cells showed an impact on the innate immune system, downregulating B‐cell population. In summary, these data provide a fascinating new and innovative approach, supporting bone healing after bacterial infections and moreover gain insights into the complex ceremony of stem cell interaction in terms of bone infection and regeneration. Stem Cells Translational Medicine 2019;8:1084–1091
format	article
author	Johannes Maximilian Wagner Felix Reinkemeier Christoph Wallner Mehran Dadras Julika Huber Sonja Verena Schmidt Marius Drysch Stephanie Dittfeld Henriette Jaurich Mustafa Becerikli Kathrin Becker Nicole Rauch Vikas Duhan Marcus Lehnhardt Björn Behr
author_facet	Johannes Maximilian Wagner Felix Reinkemeier Christoph Wallner Mehran Dadras Julika Huber Sonja Verena Schmidt Marius Drysch Stephanie Dittfeld Henriette Jaurich Mustafa Becerikli Kathrin Becker Nicole Rauch Vikas Duhan Marcus Lehnhardt Björn Behr
author_sort	Johannes Maximilian Wagner
title	Adipose‐Derived Stromal Cells Are Capable of Restoring Bone Regeneration After Post‐Traumatic Osteomyelitis and Modulate B‐Cell Response
title_short	Adipose‐Derived Stromal Cells Are Capable of Restoring Bone Regeneration After Post‐Traumatic Osteomyelitis and Modulate B‐Cell Response
title_full	Adipose‐Derived Stromal Cells Are Capable of Restoring Bone Regeneration After Post‐Traumatic Osteomyelitis and Modulate B‐Cell Response
title_fullStr	Adipose‐Derived Stromal Cells Are Capable of Restoring Bone Regeneration After Post‐Traumatic Osteomyelitis and Modulate B‐Cell Response
title_full_unstemmed	Adipose‐Derived Stromal Cells Are Capable of Restoring Bone Regeneration After Post‐Traumatic Osteomyelitis and Modulate B‐Cell Response
title_sort	adipose‐derived stromal cells are capable of restoring bone regeneration after post‐traumatic osteomyelitis and modulate b‐cell response
publisher	Wiley
publishDate	2019
url	https://doaj.org/article/c4442bc71f8d4b39976ad6bc477dcbf6
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Adipose‐Derived Stromal Cells Are Capable of Restoring Bone Regeneration After Post‐Traumatic Osteomyelitis and Modulate B‐Cell Response

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