Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases

Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases

Pinoresinol–lariciresinol reductases (PLRs) are enzymes involved in the lignan biosynthesis. Here, crystal structures of three PLRs in the apo, substrate-bound and product-bound states, and accompanying mutagenesis provide insight into PLRs catalytic mechanism and suggest a strategy for PLR engineer...

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Autores principales: Ying Xiao, Kai Shao, Jingwen Zhou, Lian Wang, Xueqi Ma, Di Wu, Yingbo Yang, Junfeng Chen, Jingxian Feng, Shi Qiu, Zongyou Lv, Lei Zhang, Peng Zhang, Wansheng Chen
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/c447762f36564f00bfa424a474de6a37
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spelling oai:doaj.org-article:c447762f36564f00bfa424a474de6a372021-12-02T15:43:05ZStructure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases10.1038/s41467-021-23095-y2041-1723https://doaj.org/article/c447762f36564f00bfa424a474de6a372021-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-23095-yhttps://doaj.org/toc/2041-1723Pinoresinol–lariciresinol reductases (PLRs) are enzymes involved in the lignan biosynthesis. Here, crystal structures of three PLRs in the apo, substrate-bound and product-bound states, and accompanying mutagenesis provide insight into PLRs catalytic mechanism and suggest a strategy for PLR engineering.Ying XiaoKai ShaoJingwen ZhouLian WangXueqi MaDi WuYingbo YangJunfeng ChenJingxian FengShi QiuZongyou LvLei ZhangPeng ZhangWansheng ChenNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Ying Xiao
Kai Shao
Jingwen Zhou
Lian Wang
Xueqi Ma
Di Wu
Yingbo Yang
Junfeng Chen
Jingxian Feng
Shi Qiu
Zongyou Lv
Lei Zhang
Peng Zhang
Wansheng Chen
Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases
description Pinoresinol–lariciresinol reductases (PLRs) are enzymes involved in the lignan biosynthesis. Here, crystal structures of three PLRs in the apo, substrate-bound and product-bound states, and accompanying mutagenesis provide insight into PLRs catalytic mechanism and suggest a strategy for PLR engineering.
format article
author Ying Xiao
Kai Shao
Jingwen Zhou
Lian Wang
Xueqi Ma
Di Wu
Yingbo Yang
Junfeng Chen
Jingxian Feng
Shi Qiu
Zongyou Lv
Lei Zhang
Peng Zhang
Wansheng Chen
author_facet Ying Xiao
Kai Shao
Jingwen Zhou
Lian Wang
Xueqi Ma
Di Wu
Yingbo Yang
Junfeng Chen
Jingxian Feng
Shi Qiu
Zongyou Lv
Lei Zhang
Peng Zhang
Wansheng Chen
author_sort Ying Xiao
title Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases
title_short Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases
title_full Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases
title_fullStr Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases
title_full_unstemmed Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases
title_sort structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/c447762f36564f00bfa424a474de6a37
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